[1]董京祥,任 丽,张 园,等.白桦BpTCPs基因家族生物信息学及时空表达分析[J].南京林业大学学报(自然科学版),2018,42(04):113-118.[doi:10.3969/j.issn.1000-2006.201709001]
 DONG Jingxiang,REN Li,ZHANG Yuan,et al.Bioinformatics and expression analysis of BpTCPs in Betula platyphylla Suk.[J].Journal of Nanjing Forestry University(Natural Science Edition),2018,42(04):113-118.[doi:10.3969/j.issn.1000-2006.201709001]
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白桦BpTCPs基因家族生物信息学及时空表达分析
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《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

卷:
42
期数:
2018年04期
页码:
113-118
栏目:
研究论文
出版日期:
2018-07-12

文章信息/Info

Title:
Bioinformatics and expression analysis of BpTCPs in Betula platyphylla Suk.
文章编号:
1000-2006(2018)04-0113-06
作者:
董京祥任 丽张 园杨 洋黄海娇李慧玉*
东北林业大学林学院,黑龙江 哈尔滨 150040
Author(s):
DONG Jingxiang REN Li ZHANG Yuan YANG Yang HUANG Haijiao LI Huiyu*
School of Forestry, Northeast Forestry University,Harbin 150040, China
关键词:
白桦 TCP基因家族 生物信息学 基因表达
Keywords:
Keywords:Betula platyphylla TCP gene family bioinformatics gene expression
分类号:
S792; Q781
DOI:
10.3969/j.issn.1000-2006.201709001
文献标志码:
A
摘要:
【目的】通过研究白桦TCP转录因子的序列特征及其在不同时期组织中的表达规律,为揭示BpTCPs 在白桦生长发育中的作用提供证据。【方法】依据白桦45个转录组测序结果,共获得 15 条TCPs 基因。利用生物信息学方法对TCPs基因进行了全面分析,采用实时荧光定量PCR技术分析基因在不同组织中的表达模式。【结果】生物信息学分析发现,15 条 BpTCPs均含有 1 个高度保守的bHLH结构域,系统进化分析发现 15条白桦TCP分属于TCP 蛋白的两大类; qRT-PCR分析结果显示,自5月到 9月的生长阶段中,顶芽中 BpTCPs 的表达水平呈现不同程度升高的变化趋势; 茎中BpTCP3在整个生长季上调表达; BpTCP4、BpTCP10及BpTCP7、BpTCP8分别在越冬后的雌花及雄花中上调表达。【结论】明确了BpTCPs基因的功能及揭示其参与调控植物生长的分子机制奠定基础。
Abstract:
Abstract: 【Objective】To analyze the sequence features and gene expression patterns of TCP transcription factors from Betula platyphylla Suk. in different tissues and time points. 【Method】Based on 45 birch transcriptome databases, 15 BpTCP genes were identified and the sequence features and gene expression at different time points and in different tissues(leaves, terminal buds, stems, male inflorescences and female inflorescences)were analyzed.【Result】Bioinformatic analysis showed that all 15 BpTCPs contained a highly conserved bHLH domain. Phylogenetic analysis showed that the 15 birch TCP proteins belonged to two major categories.The qRT-PCR results suggested that the expression levels of the 15 BpTCP genes changed significantly in terminal buds and that most BpTCP genes were up-regulated from May to September. In stems, BpTCP3 exhibited an increasing pattern during the full growth period; BpTCP4, BpTCP10, BpTCP7 and BpTCP8 were up-regulated in male and female inflorescences after winter.【Conclusion】This study may provide information for further research on the functional characteristics of BpTCPs gene and the molecular mechanism(s)involved in the regulation of plant growth.

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备注/Memo

备注/Memo:
基金项目:国家重点研发计划(2017YFD0600603) 第一作者:董京祥(dongjingxiang0519@163.com)。*通信作者:李慧玉(lihuiyu2017@1263com),教授。
更新日期/Last Update: 2018-07-27