[1]邓小梅,吴乔娜,李蕊萍,等.壳斗科植物组织培养研究进展[J].南京林业大学学报(自然科学版),2018,42(04):171-180.[doi:10.3969/j.issn.1000-2006.201609034]
 DENG Xiaomei,WU Qiaona,LI Ruiping,et al.Research progress on in vitro micropropagation of Fagaceae plants[J].Journal of Nanjing Forestry University(Natural Science Edition),2018,42(04):171-180.[doi:10.3969/j.issn.1000-2006.201609034]
点击复制

壳斗科植物组织培养研究进展
分享到:

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

卷:
42
期数:
2018年04期
页码:
171-180
栏目:
综合述评
出版日期:
2018-07-12

文章信息/Info

Title:
Research progress on in vitro micropropagation of Fagaceae plants
文章编号:
1000-2006(2018)04-0171-10
作者:
邓小梅12 吴乔娜1李蕊萍1赵梦秋1林洁莹1奚如春12䥺Symbolj@@
1.广东省森林植物种质创新与利用重点实验室,广东 广州 510642; 2.华南农业大学林学与风景园林学院,广东 广州 510642
Author(s):
DENG Xiaomei12 WU Qiaona1 LI Ruiping1 ZHAO Mengqiu1 LIN Jieying1 XI Ruchun12
1. Guangdong Key Laboratory for Innovative Development and Utilization of Forest Germplasm, Guangzhou 510642, China; 2. College of Forestry and Landscape Architecture,South China Agricultural University, Guangzhou 510642, China
关键词:
壳斗科 组织培养 基本培养基 植物生长调节剂
Keywords:
Keywords:Fagaceas tissue culture basic medium plant growth regulator
分类号:
S722.3+7; S792
DOI:
10.3969/j.issn.1000-2006.201609034
文献标志码:
A
摘要:
壳斗科(Fagaceae)植物是北半球热带、亚热带及温带森林植被的主要建群树种,在我国分布广泛,具有重大的社会经济与生态价值。植物组织培养技术是林木优良种质繁殖及无性系化推广的主要途径之一。从组培苗再生途径、外植体选择、基本培养基及植物生长调节剂应用等方面总结了壳斗科植物组织培养研究的进展,在外植体选择中应考虑到选择的诱导途径,培养基和植物生长调节剂的选择应根据不同树种在基本培养基上的生长情况做相应调整。并探讨了其组培过程中存在的污染率高、褐化严重、增殖芽伸长、生根困难等问题及解决方法,旨在进一步推动壳斗科植物组织培养的发展。
Abstract:
Abstract: The Fagaceae family includes the major tree species of the tropical, subtropical and temperate forest vegetation of the Northern Hemisphere and is widely distributed in China. Plants in this family are important in terms of their socioeconomic and ecological value. Plant tissue culture technology is one of the main methods used in promoting the excellent germplasm and asexual reproduction. This paper reviews progress made in studies of the micropropagation techniques used for Fagaceae plants, in terms of explant selection, basic media and the types and concentrations of plant growth regulator. Insights include that induction pathways should be considered in the selection of explants, and that different species require adjustments in the selection of media and plant growth regulators. Furthermore, several problems in recent studies and their solutions have been discussed, such as contamination, browning and the difficulties experienced in rooting and elongation of proliferation buds. All of this research is aimed at promoting tissue culture in Fagaceae plants.

参考文献/References:

[1] 周浙昆. 壳斗科的地质历史及其系统学和植物地理学意义[J]. 植物分类学报, 1999, 37(4): 369-385. ZHOU Z K. Fossils of the Fagaceae and their implications in systematics and biogeography[J]. Acta Phytotaxonomica Sinica, 1999, 37(4): 369-385.
[2] GOVAERTS R,FRODIN D G. World checklist and bibliography of Fagales[M]. London: the Royal Botanic Gardens,Kew, 1998: 201-204.
[3] 刘茂松,洪必恭. 中国壳斗科的地理分布及其与气候条件的关系[J]. 植物生态学报, 1998(1): 42-43. LIU M S,HONG B G. The distribution of Fagaceae in China and its relationgship with climatic and geographic charachers[J]. Acta Phytoecologica Sinica, 1998(1): 42-43.
[4] 罗坤水,杨春霞,林小凡,等. 壳斗科树种育苗技术研究[J]. 江西林业科技, 2008(6): 6-9. LUO K S, YANG C X, LIN X F, et al. Research on nursery technology of Fagaceae[J]. Jiangxi Forestry Science and Technology, 2008(6): 6-9.
[5] 金振洲,区普定. 我国的硬叶常绿阔叶林[J]. 云南大学学报(自然科学版), 1981(2): 13-20. JIN Z Z, OU P D. Hard-leaved evergreen broad-leaved forest in China[J]. Journal of Yunnan University(Natural Science Edition), 1981(2): 13-20.
[6] 许瑾. 壳斗科植物在我国城市园林绿化中的应用现状及前景[J]. 现代园艺, 2013(17): 37-38.DOI: 10.3969/j.issn.1006-4958.2013.17.025. XU J. Application status and prospect of Fagaceae plants in urban landscaping in China[J]. Modern Gardening, 2013(17): 37-38.
[7] 吴丽君. 木本植物组织培养技术在林业科研与生产中的应用与局限[J]. 福建林业科技, 2003(1): 67-69.DOI: 10.3969/j.issn.1002-7351.2003.01.017. WU L J. Application and limitations of the woody plant tissue culture technique in the forestry scientific research and production[J]. Jour of Fujian Forestry Sci and Tech, 2003(1): 67-69.
[8] 邓小梅. 乐东拟单性木兰、华木莲、红叶石楠‘红罗宾'的组织培养及快繁技术研究[D]. 南京:南京林业大学, 2004. DENG X M. A study on tissue cuiture and rapid propagation of Parakmeria lotungonsis(Chun et C.Tsoong)Law, Sinomanglietia glauca Z.X.Yu et Q.Y.Zhang and Photinia×fraseri ‘Red Robin'[D]. Nanjing:Nanjing Forestry University, 2004.
[9] JAQUIOT C. Sur les phénomènes d'histogenèse observés dans les cultures in vitro de tissu cambial de chènes(Quercus sessiflora Sm., Q. pedunculata, Ehr., Q. suber L.)[J]. C R Hebd Seances Acad Sci, 1952, 234: 1468-1470.
[10] 宋敏. 栓皮栎离体培养再生植株的研究[D]. 杨凌:西北农林科技大学, 2003. SONG M.Regeneration plantiet for Quercus variabilis in vitro[D]. Yangling:Sorthwest Sei-Tech University of Agriculture and Forestry, 2003.
[11] 任鹏,郭素娟. 栗属树种离体培养研究进展[J]. 经济林研究, 2004,22(2): 65-68. DOI:10.3969/j.issn.1003-8981.2004.02.023. REN P, GUO S J. Advances of research on propagation of chestnut in vitro[J]. Nonwood Forest Research, 2004,22(2): 65-68.
[12] 廖婧. 麻栎体细胞胚胎发生与快速繁殖技术研究[D]. 南京:南京林业大学, 2012. LIAO J. Somatic embryogenesis and rapid propagation technology of Quercus acutissima Carr.[D]. Nanjing: Nanjing Forestry University, 2012.
[13] 赵丹.麻栎组培和扦插繁殖技术研究[D]. 南京:南京林业大学, 2010. ZHAO D. The research of propagation technique of tissue culture and cutting of Quercus acutissima[D]. Nanjing:Nanjing Forestry University, 2010.
[14] PINTO G,VALENTIM H,COSTA A,et al. Somatic embryogenesis in leaf callus from a mature Quercus suber L. tree[J]. In Vitro Cellular & Developmental Biology-Plant, 2002, 38(6): 569-572. DOI: 10.1079/IVP2002352 1054-5476/02.
[15] MARTÍNEZ M,SAN JOSÉ M,VIEITEZ A,et al. Propagation of mature Quercus ilex L.(holm oak)trees by somatic embryogenesis[J]. Plant Cell, Tissue and Organ Culture, 2017, 131(2): 321-333.
[16] VENGADESAN G,PIJUT P M. In vitro propagation of northern red oak(Quercus rubra L.)[J]. In Vitro Cellular & Developmental Biology-Plant, 2009, 45(4): 474-482.. DOI:10.1007/s11627-008-9182-6.
[17] VIEITEZ A,CORREDOIRA E,BALLESTER A,et al. In vitro regeneration of the important North American oak species Quercus alba, Quercus bicolor and Quercus rubra[J]. Plant Cell, Tissue and Organ Culture, 2009, 98(2): 135-145.
[18] 魏爽,崔建国,温伟,等. 辽东栎的组织培养和植株再生[J]. 植物生理学通讯, 2010, 46(12): 1277-1278. WEI S, CUI J G, WEN W,et al. Tissue culture and plantlet regeneration of Quercus liaotungensis Klidz[J]. Plant Physiology Newsletter, 2010, 46(12): 1277-1278.
[19] CHALUPA V.In vitro propagation of oak(Quereus robur L.)and linden(Tilia cordata Mill.)[J]. Biologia Plantarum, 1984, 26(5): 374-377. DOI:10.1007/bf02898577.
[20] JUNCKER B,FAVRE J. Clonal effects in propagating oak trees via in vitro culture[J]. Plant Cell, Tissue and Organ Culture, 1989, 19(3): 267-276. DOI:10.1007/bf00043353.
[21] 丁世民,李晓娟,于颖,等. 沈氏栎组培快繁技术的初步研究[J]. 安徽农业科学, 2012, 40(23): 11606-11608.DOI: 10.3969/j.issn.0517-6611.2012.23.028. DING S M, LI X J, DING Y,et al. Preliminary study on the tissue culture and rapid propagation of Quercus shumardi ‘Shumard Oak'[J]. Journal of Anhui Agri Sci, 2012, 40(23): 11606-11608.
[22] 李文文,刘希华,黄秦军,等. 蒙古栎茎段的离体培养[J]. 东北林业大学学报, 2012, 40(8): 1-6.DOI: 10.3969/j.issn.1000-5382.2012.08.001. LI W W,LIU X H,HUANG Q J, et al. In vitro cultivation of nodal segments in Quercus mongolica[J]. Journal of Northeast Forestry University, 2012, 40(8): 1-6.
[23] 高珊,林梅,崔建国,等. 蒙古栎促萌茎段的离体培养研究[J]. 北方园艺, 2013(19): 102-105. GAO S, LIN M, CUI J G, et al. Study on the in vitro culture of budding stem segments of Quercus mongolica[J]. Northern Horticulture, 2013(19): 102-105.
[24] 赵吉胜,杨晶. 蒙古栎组织培养的初步研究[J]. 吉林林业科技, 2014,43(1): 17-19.DOI:10.3969/j.issn.1005-7129.2014.01.004. ZHAO J S,YANG J. Study on tissue culture of Quercus mongolica[J].Journal of Jilin Forestry Science and Technology, 2014,43(1): 17-19.
[25] 吕秀立,沈烈英,施季森,等. 苏玛栎离体培养和植株再生研究[J]. 植物研究, 2015, 35(2): 185-190. LV X L, SHEN L Y, SHI J S, et al.In vitro culture and plantlet regeneration of Quercus shumardii[J]. Bulletin of Botanical Research, 2015,35(2): 185-190.
[26] 吴幼媚,陈晓明,王以红,等. 大叶栎的组织培养和快速繁殖[J]. 植物生理学通讯, 2008,44(1): 114. WU Y M, WANG X M, WANG Y H, et al. Tissue culture and rapid propagation of Castanopsis fissa(Champ. Ex Benth.)Rehd.et WilS[J]. Plant Physiology Newsletter, 2008,44(1): 114.
[27] 王以红,陈晓明,蔡玲,等. 大叶栎组织培养再生植株的研究[J]. 广西林业科学, 2009,38(2): 71-74.DOI:10.3969/j.issn.1006-1126.2009.02.001. WANG Y H, CHEN X M, CAI L, et al. Study on tissue culture and regeneration of Castanopsis fissa [J]. Guangxi Forestry Science, 2009,38(2): 71-74.
[28] 杨国臣,鲁重格,THEOPHILUS M A,等. 美洲板栗愈伤组织诱导和植株再生的研究[J]. 吉林农业大学学报, 2008, 30(4): 466-471. YANG G C, LU Z G, THEOPHILUS M A, et al.In vitro callus induction and shoot initiation of American chestnut(Castanea dentata)[J].Journal of Jilin Agricultural University, 2008, 30(4): 466-471.
[29] 刘玉芬,赵国强,曹庆芹,等. 板栗短雄花序芽变的离体培养研究[J]. 中国农学通报, 2008,24(10): 99-102. LIU Y F, ZHAO G Q, CAO Q Q, et al. Propagation in vitro of chestnut short catkin mutant(Castanea mollissima Bl.)[J]. Chinese Agricultural Science Bulletin, 2008,24(10): 99-102.
[30] 徐晨,陈双双,王金金,等. 板栗高效组培体系的建立和优化[J]. 北京农学院学报, 2013, 28(3): 6-9. XU C, CHEN S S, WANG J J, et al. Establishment and optimization of efficient micropropagation system in Castanea mollissima[J]. Journal of Beijing University of Agriculture, 2013, 28(3): 6-9.
[31] 郭素娟,孙小兵,秦天天,等. 板栗成熟胚再生体系的建立与优化[J]. 西北林学院学报, 2015, 30(3): 89-93.DOI: 10.3969/j.issn.1001-7461.2015.03.16. GUO S J, SUN X B, QIN T T,et al. Establishment and optimi-zation of in vitro regeneration system of mature embryo of Castanea mollissima[J]. Journal of Northwest Forestry University, 2015, 30(3): 89-93.
[32] 冯金玲. 锥栗组织培养及其再生体系建立的研究[D]. 福州: 福建农林大学, 2004. FENG J L. Study on tissue culture and regeneration system of Cstanea henryi[D]. Fuzhou: Fujian Agriculture and Forestry University, 2004.
[33] 杨志坚,冯金玲,陈辉. 锥栗植株再生体系的建立[J]. 果树学报, 2013, 30(1): 105-109. YANG Z J, FENG J L, CHEN H. Establishment of tissue culture regeneration system for Castanea henryi[J]. Journal of Fruit Science, 2013,30(1): 105-109.
[34] 黄光文,张平,张志光. 柯的组织培养[J]. 零陵学院学报, 2002,23(6): 81-83. HUANG G W, ZHANG P, ZHANG Z G. The organizational culture of Lithocarpus glaber(Thunb.)Nakai [J].Journal of Linling University, 2002,23(6): 81-83.
[35] 孙卫邦,周元,向其柏,等. 三棱栎的组培方法: CN1899028[P]. 2007-01-24.
[36] 汪丽. 赤皮青冈快繁技术研究[D]. 长沙:中南林业科技大学, 2014. WANG L. Cyclobalanopsis gilva rapid propagation technology research[D]. Changsha:Central South University of Forestry and Technology, 2014.
[37] 王艳娟. 赤皮青冈组织培养技术研究[D]. 长沙:中南林业科技大学, 2015. WANG Y J. The technology research for tissue culture of Cyclobalanopsis gilva(Blume)Oerst[D]. Changsha:Central South University of Forestry and Technology, 2015.
[38] BONGA J,KLIMASZEWSKA K,VON ADERKAS P. Recalcitrance in clonal propagation, in particular of conifers[J]. Plant Cell, Tissue and Organ Culture, 2010, 100(3): 241-254. DOI 10.1007/s11240-009-9647-2.
[39] 胡莹,杨柳青. 山茶科植物组织培养研究进展[J]. 江苏农业科学, 2008(2): 6-9. DOI:10.3969/j.issn.1002-1302.2008.02.002. HU Y, YANG L Q. Research progress on tissue culture of Camellia Plants[J]. Jiangsu Agricultural Sciences, 2008,(2): 6-9.
[40] 陈建华,罗丽华,苏冬梅,等. 不同板栗品种的组培技术[J]. 中南林业科技大学学报, 2007, 27(6): 83-87.DOI: 10.3969/j.issn.1673-923X.2007.06.013. CHEN J H, LUO L H, SU D M, et al. The technique of tissue culture for different varieties of chestnut[J]. Journal of Central South University of Forestry &Technology, 2007,27(6): 83-87.
[41] 唐罗忠,赵丹,诸葛强,等. 麻栎组织培养外植体选择与灭菌方法[J]. 江苏林业科技, 2010, 37(5): 22-25. DOI: 10.3969/j.issn.1001-7380.2010.05.006 TANG L Z,ZHAO D,ZHU G Q,et al. Explants selection and sterilization method of tissue culture for Quercus acutissima[J]. Journal of Jiangsu Forestry Science & Technology, 2010, 37(5): 22-25.
[42] 任鹏. 板栗(Castanea mollissima BL.)组织培养技术研究[D]. 北京:北京林业大学, 2005. REN P. Study on micropropagation of Chinese chestnut in vitro[D]. Beijing:Beijing Forestry University, 2005.
[43] 张存旭. 栓皮栎体细胞胚胎发生及生化特性的研究[D]. 杨凌:西北农林科技大学,2007. ZHANG C X. Somatic embryogenesis and biochemical characteristics in Quercus variabilis Bl[D]. Yangling: Northwest A&F University, 2007.
[44] 杨锋利. 成龄栓皮栎组培繁殖技术的研究[D]. 杨凌:西北农林科技大学,2005. YANG F L. In vitro propagation of mature trees of Quercus variabilis Bl[D]. Yangling:Northwest A & F University, 2005.
[45] CHALUPA V. Large scale micropropagation of Quercus robur L. using adenine-type cytokinins and thidiazuron to stimulate shoot proliferation[J]. Biologia Plantarum,1988,30(6): 414-421. DOI:10.1007/bf02890509.
[46] 苏冬梅,罗丽华,杨定海. 板栗愈伤组织的诱导[J]. 经济林研究,2004,22(3): 17-19. SU D M, LUO L H, YANG D H. Research on callus induction of Chinese chestnut[J]. Nonwood Forest Research, 2004,22(3): 17-19.
[47] 魏爽. 辽东栎合子胚的离体培养[D]. 沈阳:沈阳农业大学,2011. WEI S. In vitro culture of zygotic embryoin in Quercus liaotungensis Koidz[D]. Shenyang:Shenyang Agricultural University, 2011.
[48] 覃子海,刘海龙,蒋华,等. 红锥组织培养与快速繁殖的研究[J]. 热带农业科学,2014,34(2): 50-53. QIN Z H,LIU H L,JIANG H,et al. Tissue culture and rapid multiplication of Castanopsis hystrix[J]. Chinese Journal of Tropical Agriculture,2014,34(2): 50-53.
[49] JOHNSON K R,WALKER R F. Micropropagation of valley oak shoots from seedling explants[J]. New Forests,1991,4(4): 271-279. DOI:10.1007/bf00119206.
[50] 张存旭,宋敏,赵忠. 栓皮栎茎段离体培养的研究[J]. 西北植物学报,2004,24(7): 1260-1265. ZHANG C X, SONG M, ZHAO Z. In vitro cultivation of nodal segments of the cork tree(Quercus variabilis)[J]. Acta Bot Boreal-Occident Sin, 2004,24(7): 1260-1265.
[51] 李玲莉. 柔枝松和红锥的组织培养研究[D]. 北京:北京林业大学,2010. LI L L. Study on tissue culture of Pinus flexilis and Castanopsis hystrix A. DC[D]. Beijing:Beijing Forestry University, 2010.
[52] 于艳,郎庆龙,夏兴宏,等. 以多年生麻栎茎段为外植体的组织培养条件优化[J]. 蚕业科学,2014,40(2): 202-209. YU Y,LANG Q L,XIA X H,et al. Optimization of tissue culture condition using perennial Quercus acutissima stem segments as explant[J]. Science of Sericulture,2014,40(2): 202-209.
[53] 卫梅. 闽西青冈组织培养体系的建立及其机理研究[D].福州: 福建农林大学,2016. WEI M. Study on tissue culture technology and mechanism of Cyclobalanopsis minxiensis[D]. Fuzhou: Fujian Agriculture and Forestry University, 2016.
[54] 覃子海,覃鹏飞,吴幼媚,等. 红锥不同外植体消毒技术初探[J]. 生物技术世界,2013(8): 15-16. QIN Z H, QIN P F, WU Y M, et al. Study on disinfection techniques of Castanopsis hystrix from different explants[J]. Biotechnology World, 2013(8): 15-16.
[55] 孙小兵,郭素娟. 成龄板栗组培快繁体系的建立及影响因素的研究[J]. 中南林业科技大学学报,2015,35(4): 51-55. SUN X B,GUO S J. Establishment and influencing factors of micropropagation system in mature Castanea mollissima[J]. Journal of Central South University of Forestry & Technology,2015,35(4): 51-55.
[56] XING Z,SATCHWELL M F,POWELL W A,et al. Micropropagation of American chestnut: increasing rooting rate and preventing shoot-tip necrosis[J]. In Vitro Cellular & Developmental Biology Plant, 1997, 33(1): 43-48.
[57] 张玲,尹伟伦,王华芳. 板栗胚珠培养研究初报[J]. 北京林业大学学报,2007,29(5): 99-105. ZHANG L, YIN W L, WANG H F. Ovule culture in vitro of Castanea mollissima Bl[J]. Journal of Beijing Forestry University, 2007,29(5): 99-105.
[58] SAN-JOSÉ M C,CORREDOIRA E,MARTÍNEZ M T,et al. Shoot apex explants for induction of somatic embryogenesis in mature Quercus robur L. trees[J]. Plant Cell Reports, 2010, 29(6): 661-671.
[59] 陈双双,曹庆芹,邢宇,等. 板栗愈伤组织诱导[C]. 广州:中国园艺学会2013年学术年会, 2013: 2603.
[60] CORREDOIRA E,VALLADARES S,VIEITEZ A M. Morphohistological analysis of the origin and development of somatic embryos from leaves of mature Quercus robur[J]. In Vitro Cellular & Developmental Biology-Plant,2006,42(6): 525-533. DOI:10.1079/ivp2006827.
[61] PUROHIT V K,TAMTA S,CHANDRA S,et al. In vitro multiplication of Quercus leucotrichophora and Q. glauca: important Himalayan oaks[J]. Plant Cell, Tissue and Organ Culture, 2002, 69(2): 121-133.
[62] 金建邦,祝遵凌. 桦木科植物组织培养研究进展[J]. 北方园艺,2013(23): 202-206. JIN J B, ZHU Z L. Review of birch families tissue culture[J]. Northern Horticulture, 2013(23): 202-206.
[63] 王桂云. 燕山红栗茎尖组织培养及不定根发生机理研究[D]. 北京:北京林业大学,2007. WANG G Y. Studies on tissue culture using shoot tips and adventitious root mechanism[D]. Beijing:Beijing Forestry University 2007.
[64] HERNÁNDEZ I,CELESTINO C,ALEGRE J,et al. Vegetative propagation of Quercus suber L. by somatic embryogenesis. II. plant regeneration from selected cork oak trees[J]. Plant Cell Rep,2003,21(8): 765-770. DOI:10.1007/s00299-003-0604-y.
[65] 冯金玲,陈辉,杨志坚,等. 锥栗组织培养外植体消毒和选择[J]. 福建林学院学报,2006,26(1): 22-25.DOI: 10.13324/j.cnki.jfcf.2006.01.006. FENG J L, CHEN H, YANG Z J, et al. The selection and sterilization of explant of Castanea henryiin tissue culture[J]. Journal of Fujian College of Forestry, 2006, 26(1): 22-25.
[66] 朱登云,李浚明. 植物组织培养教程 [M].3版. 北京:中国农业大学出版社,2006.
[67] 谢志亮,吴振旺. 木本植物组培褐化研究进展[J]. 中国南方果树,2013,42(5): 42-46.DOI:10.13938/j.issn.1007-1431.2013.05.001. XIE Z L, WU Z W. The review on browning in woody plant tissue culture[J]. South China Fruits, 2013, 42(5): 42-46.
[68] 刘光金,黄寿先,刘芳,等. 大叶栎组织培养中外植体褐变的初步研究[J]. 安徽农业科学,2009,37(16): 7343-7344. LIU G J, HUANG S X, LIU F, et al. Preliminaty study on browning of explant in Castanopsin fissa tissue culture[J]. Journal of Anhui Agri Sci, 2009, 37(16): 7343-7344.
[69] 周冰彬,陈晓阳,李慧. 不同培养条件对黧蒴栲组培苗褐变的抑制作用[J]. 河北林果研究,2007,22(4): 348-350,354. ZHOU B B, CHEN X Y, LI H. Effect of different conditions on browning of Castanopsis fissa in tissue culture[J]. Hebei Journal of Forestry and Orchard Research, 2007, 22(4): 348-350,354.
[70] 丁彤. 北美红栎无性繁殖体系的研究[D]. 合肥:安徽农业大学,2012. DING T. Study on the asexual reproduction system of northern red oak[D]. Hefei:Anhui Agriculture University, 2012.
[71] 刘杜玲,吕平会,彭少兵,等. 板栗愈伤组织诱导及外植体褐化研究[J]. 中南林业科技大学学报,2016,36(12): 26-30. LIU D L, LV P H, PENG S B, et al. Research on callus induction and explant browning of chestnut(Castanea mollissima Bl.)[J]. Journal of Central South University of Forestry & Technology, 2016, 36(12): 26-30.
[72] 于艳,夏兴宏,石淑萍,等. 减少多年生麻栎茎段外植体褐化的材料采集与组织培养条件优化[J]. 蚕业科学,2015,41(5): 793-800. YU Y, XIA X H, SHI S P, et al. Material collection of stem segment explants from perennial Quercus acutissima and optimization of tissue culture condition to reduce browning[J]. Science of Sericulture, 2015, 41(5): 793-800.
[73] 董胜君,刘明国,郑可,等. 基于幼化效果的山杏组培繁殖技术研究[J]. 西北植物学报,2017,37(3): 595-601. DONG S J, LIU M G, ZHENG K, et al. Tissue culture propagation technique of Armeniaca sibirica based on rejuvenation effect[J]. Acta Bot Boreal-Occident Sin, 2017, 37(3): 595-601.
[74] 张世红,李坤霞,朱淑新,等. 毛白杨成熟效应与组培幼化[J]. 西北林学院学报,2010,25(4): 83-86. ZHANG S H, LI K X, ZHU S X, et al. Cycolphysis and rejuvenation of Populus tomentosa by tissue culture[J]. Journal of Northwest Forestry University, 2010, 25(4): 83-86.
[75] 孙立平,章林,赵珊珊,等. 大叶山杨幼化处理技术的研究[J]. 吉林林业科技,2009,38(5): 18-20. SUN L P, ZHANG L, ZHAO S S, et al. Research on the rejuvenation technology of Populus davidiana var.macrophylla[J]. Jilin Forestry Science and Technology, 2009, 38(5): 18-20.
[76] 邓建军,李芳东,乔杰,等. 白花泡桐优树试管嫁接幼化及组培快繁技术研究[J]. 林业科学研究,2011,24(5): 646-650. DENG J J, LI F D, QIAO J, et al. Study on grafted tissue rejuvenation technology and rapid propagation of Paulownia fortunei superior trees[J]. Forest Research, 2011,24(5): 646-650.

相似文献/References:

[1]季孔庶.杂交鹅掌楸的无性繁殖[J].南京林业大学学报(自然科学版),2005,29(01):083.[doi:10.3969/j.jssn.1000-2006.2005.01.020]
 JI Kong-shu.Cionai Propagation of Liriodendron chinense×L. tulipi f era[J].Journal of Nanjing Forestry University(Natural Science Edition),2005,29(04):083.[doi:10.3969/j.jssn.1000-2006.2005.01.020]
[2]陈颖,曹福亮*.稀土对银杏组培芽苗生长及其不定根的影响[J].南京林业大学学报(自然科学版),2005,29(06):054.[doi:10.3969/j.jssn.1000-2006.2005.06.013]
 CHEN Ying,CAO Fu-liang*.Effects of Rare Earth on Growth of Cultured Terminal Buds and on Induction of Adventitious Roots of Ginkgo biloba In Vitro[J].Journal of Nanjing Forestry University(Natural Science Edition),2005,29(04):054.[doi:10.3969/j.jssn.1000-2006.2005.06.013]
[3]李杰,黄敏仁,王明庥,等.兰花生物技术的研究及应用[J].南京林业大学学报(自然科学版),2006,30(02):108.[doi:10.3969/j.jssn.1000-2006.2006.02.026]
 LI Jie,HUANG Min-ren,WANG Ming-xiu,et al.Research and Application on the Biotechnique Engineers in Orchids[J].Journal of Nanjing Forestry University(Natural Science Edition),2006,30(04):108.[doi:10.3969/j.jssn.1000-2006.2006.02.026]
[4]吕月良,陈璋,施季森*,等.福建山樱花不定芽诱导和植株再生规模化繁殖试验[J].南京林业大学学报(自然科学版),2006,30(03):105.[doi:10.3969/j.jssn.1000-2006.2006.03.024]
 Lv Yue-liang,CHEN Zhang,SHI Ji sen*,et al.Adventitious Bud Inducing and Plant Regeneration of Cerasus campanulata Maxim. in Large Scale[J].Journal of Nanjing Forestry University(Natural Science Edition),2006,30(04):105.[doi:10.3969/j.jssn.1000-2006.2006.03.024]
[5]宋建英,叶建仁*.“马可”百合组培技术研究[J].南京林业大学学报(自然科学版),2006,30(04):073.[doi:10.3969/j.jssn.1000-2006.2006.04.017]
 SONG Jian-ying,YE Jian-ren*.Study on Tissue Culture of Lilium bulbiferum cv. ’Marco polo’[J].Journal of Nanjing Forestry University(Natural Science Edition),2006,30(04):073.[doi:10.3969/j.jssn.1000-2006.2006.04.017]
[6]邹永梅,施季森*.北美悬铃木的组织培养[J].南京林业大学学报(自然科学版),2006,30(06):061.[doi:10.3969/j.jssn.1000-2006.2006.06.014]
 ZOU Yong-mei,SHI Ji-sen*.Tissue Culture and Mass Propagation of Platanus occidentalis Linn[J].Journal of Nanjing Forestry University(Natural Science Edition),2006,30(04):061.[doi:10.3969/j.jssn.1000-2006.2006.06.014]
[7]宿静,汤庚国*,王光萍,等.美国月桂樱的组织培养及植株再生[J].南京林业大学学报(自然科学版),2008,32(04):098.[doi:10.3969/j.jssn.1000-2006.2008.04.022]
 SU Jing,TANG Geng-guo *,WANG Guang-ping,et al.Tissue culture and plant regeneration of Laurocerasus caroliniana Ait.[J].Journal of Nanjing Forestry University(Natural Science Edition),2008,32(04):098.[doi:10.3969/j.jssn.1000-2006.2008.04.022]
[8]刘茂松,洪必恭.中国壳斗科的分布格局类型分析[J].南京林业大学学报(自然科学版),1999,23(05):018.[doi:10.3969/j.jssn.1000-2006.1999.05.004]
 Liu Maosong Hong Bigong(Nanjing Forestry University Nanjing 007).The Analysis of Distribution Pattern of Fagaceae in China[J].Journal of Nanjing Forestry University(Natural Science Edition),1999,23(04):018.[doi:10.3969/j.jssn.1000-2006.1999.05.004]
[9]史港影,南程慧,伊贤贵,等.雪落樱再生体系的建立[J].南京林业大学学报(自然科学版),2014,38(增刊):020.[doi:10.3969/j.issn.1000-2006.2014.S1.005]
 SHI Gangying,NAN Chenghui,YI Xiangui,et al.Establishment of regeneration system in Cerasus xueluoensis[J].Journal of Nanjing Forestry University(Natural Science Edition),2014,38(04):020.[doi:10.3969/j.issn.1000-2006.2014.S1.005]
[10]官锦燕,谭嘉娜,罗剑飘,等.牛樟的组织培养和植株再生[J].南京林业大学学报(自然科学版),2016,40(04):063.[doi:10.3969/j.issn.1000-2006.2016.04.010]
 GUAN Jinyan,TAN Jiana,LUO Jianpiao,et al.Tissue culture and plant regeneration of Cinnamomum kanehirae Hay[J].Journal of Nanjing Forestry University(Natural Science Edition),2016,40(04):063.[doi:10.3969/j.issn.1000-2006.2016.04.010]

备注/Memo

备注/Memo:
基金项目:广东省省级科技计划项目(2016B020233001); 广东省林业科技创新项目(2014KJCX002) 第一作者:邓小梅(dxmei2006@scau.edu.cn),教授。吴乔娜(1009375829@qq.com)。*通信作者:奚如春(xirc2006@scau.edu.cn),教授。
更新日期/Last Update: 2018-07-27