[1]吴丽君,高 楠,陈 达,等.金花茶杂交种子无菌苗的组培快繁研究[J].南京林业大学学报(自然科学版),2018,42(05):032-38.[doi:10.3969/j.issn.1000-2006.201709042]
 WU Lijun,GAO Nan,CHEN Da,et al.Tissue culture and rapid propagation of sterile seedling of Camellia nitidissima C. W. Chi hybrid germplasm[J].Journal of Nanjing Forestry University(Natural Science Edition),2018,42(05):032-38.[doi:10.3969/j.issn.1000-2006.201709042]
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金花茶杂交种子无菌苗的组培快繁研究
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《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

卷:
42
期数:
2018年05期
页码:
032-38
栏目:
研究论文
出版日期:
2018-09-15

文章信息/Info

Title:
Tissue culture and rapid propagation of sterile seedling of Camellia nitidissima C. W. Chi hybrid germplasm
文章编号:
1000-2006(2018)05-0032-07
作者:
吴丽君1高 楠1陈 达1陈文荣2
1.福建省林业科学研究院,福建 福州 350012; 2.福建省国有来舟林业试验场,福建 南平 353004
Author(s):
WU Lijun1 GAO Nan1 CHEN Da1 CHEN Wenrong2
1. Fujian Academy of Forestry Sciences, Fuzhou 350012, China; 2. Laizhou Country Forest Farm of Fujian Province, Nanping 353004, China
关键词:
金花茶杂交 无菌播种苗 组培快繁 增殖 生根率 移栽成活率
Keywords:
Camellia nitidissima C. W. Chi hybrid germplasm sterile seedling tissue culture and rapid propagation proliferation rooting rate transplantation survival rate
分类号:
S723.1+32.1; S723.2
DOI:
10.3969/j.issn.1000-2006.201709042
文献标志码:
A
摘要:
【目的】建立金花茶杂交种质组培快繁技术体系,为优质杂交种质的保存和快繁提供技术支撑。【方法】以凹脉金花茶♀ב烈香'茶花♂(S1)、‘黄樽'薄叶金花茶♀×防城金花茶♂(S2)、防城金花茶♀×越南抱茎茶♂(S3)等3组杂交组合种子的无菌播种苗为材料,研究不同外植体诱导率的差异,杂交组合、基本培养基、生长调节剂种类浓度组合对芽苗增殖和生根的影响,蔗糖浓度对生根率的影响,及不同基质移栽成活率的差异等组培关键技术。【结果】S1、S2、S3等3组杂交组合无菌苗的顶芽平均诱导率为12%,茎段平均诱导率最高,达100%。杂交组合、基本培养基和6-BA对增殖倍数影响极显著,S1、S2、S3杂交组合在培养基B5+6-BA 2.5 mg/L+IBA 0.05 mg/L上培养40 d,增殖倍数最高,分别为7.4、6.6、7.5倍,芽苗长势好; 在MS培养基上芽苗长势不佳,叶片脱落。杂交组合、生根剂种类浓度组合及蔗糖浓度对生根率影响极显著,S1、S2、S3杂交组合在培养基1/2 B5+ABT 1.0 mg/L+5 g/L蔗糖上,生根率最高,分别为92%、85%、89%。3组杂交组合在V黄心土:V蛭石=3:1基质上的平均移栽成活率最高,达94%。【结论】无菌播种苗的茎段是金花茶杂交种质离体培养最理想的外植体,最佳增殖和生根培养基分别为B5+6-BA 2.5 mg/L+IBA 0.05 mg/L和1/2 B5+ABT 1.0 mg/L+蔗糖5 g/L,最佳移栽基质为V黄心土:V蛭石=3:1,营建组培快繁技术体系是金花茶杂交种质保存和植株高效再生的理想途径。
Abstract:
【Objective】To establish a technological system of tissue culture and rapid propagation of hybrid germplasm of Camellia nitidissima C.W. Chi that would provide technical support for the preservation and rapid propagation of elite hybrid germplasm of C. nitidissima C.W. Chi.【Method】With sterile seedlings from hybrid seeds of C. impressinervis Chang et S.Y. Liang♀× C. japonica ‘Dormi'♂(S1), C. chrysanthoides ‘Huangzun'♀ × C. chrysantha var. longistyla ♂(S2), and C. chrysantha var. longistyla ♀ × C. amplexicaulis ♂(S3)as material, we determined the difference in induction rate from different explants, including the apical bud, stem section, and radicle of a sterile seedling, effects of 3 hybrids, effects of 4 basic media including MS, B5, Hyponex No. 1, and 1/2MS+Hyponex No. 1, effects of concentration combinations of different plant growth regulators(PGRs), including 6-BA, IBA, and NAA on the proliferation and rooting of shoots, effects of sucrose concentration on rooting rate of shoots, and the difference in transplantation survival rate of 3 hybrids on 4 substrates, including pure yellow soil, 3Vpure yellow soil+1Vvermiculite, 3Vpure yellow soil+1Vperlite , and 3Vpure yellow soil+1Vcoconut .【Result】The average induction rate of apical bud and stem section of the 3 hybridized combinations was 12% and 100%, respectively. The difference in proliferation times of shoots was significant for the 3 hybridized combinations, the 4 basic media, and the different concentrations of 6-BA. The proliferation times of S1,S2 and S3 were higher at 7.4, 6.6 and 7.5, respectively, on the subculture medium B5+6-BA 2.5 mg/L+IBA 0.05 mg/L+30 g/L sucrose for 40 d. The shoots grew poorly with falling leaves on the basic medium MS for several generations of multiplication. The difference in the rooting rate of the 3 hybrids was significant on concentration combinations of different PGRs and sucrose concentration, and the higher the sucrose concentration, above 10 g/L, the lower the rooting rate. A higher rooting rate for S1,S2 and S3 was obtained on the medium 1/2 B5+ABT 1.0 mg/L+sucrose 5 g/L, at 92%, 85% and 89%, respectively. The difference in the transplantation survival rate of shoots was significant on the 4 substrates, with the average survival rate of S1, S2 and S3 reaching 94.0% on substrote of 3Vyellow soil+1Vvermiculite after hardening for 30 d.【Conclusion】Stem sections of sterile seedlings were the best explants for tissue cultures of the 3 hybridized combinations of C. nitidissima C.W. Chi. The best subculture and rooting medium was B5+6-BA 2.5 mg/L+IBA 0.05 mg/L+30 g/L sucrose and 1/2 B5+ABT 1.0 mg/L+sucrose 5 g/L, respectively. The optimum transplant mixture was 3Vyellow soil+1Vvermiculite. Tissue culture technology was the best preservation method and method for plantlet regeneration for the elite hybrid germplasm of C. nitidissima C.W. Chi.

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备注/Memo

备注/Memo:
收稿日期:2017-09-18 修回日期:2018-06-29 基金项目:福建省林业厅花卉种质创新项目(H2014019) 第一作者:吴丽君(370709128@qq.com),教授级高级工程师,博士。
更新日期/Last Update: 2018-09-15