[1]陈燕琼,沈,范佳露,等.茉莉酸甲酯对干旱及复水下落叶冬青苗叶片抗氧化水平的影响[J].南京林业大学学报(自然科学版),2018,42(06):035-43.[doi:10.3969/ j.issn.1000-2006.201712035]
 CHEN Yanqiong,SHEN Yang,FAN Jialu,et al.The effects of methyl jasmonic acid on leaf antioxidative capacity of Ilex verticillata L. seedlings under drought and re-watering[J].Journal of Nanjing Forestry University(Natural Science Edition),2018,42(06):035-43.[doi:10.3969/ j.issn.1000-2006.201712035]
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茉莉酸甲酯对干旱及复水下落叶冬青苗叶片抗氧化水平的影响
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《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

卷:
42
期数:
2018年06期
页码:
035-43
栏目:
研究论文
出版日期:
2018-12-15

文章信息/Info

Title:
The effects of methyl jasmonic acid on leaf antioxidative capacity of Ilex verticillata L. seedlings under drought and re-watering
作者:
陈燕琼1212范佳露13陈 茜12许欣玥13张 迪13钱 钢13陈 颖12*
(1.南京林业大学,南方现代林业协同创新中心,江苏 南京 210037; 2.南京林业大学生物与环境学院, 江苏 南京 210037; 3.南京林业大学林学院, 江苏 南京 210037)
Author(s):
CHEN Yanqiong12 SHEN Yang12 FAN Jialu13 CHEN Xi12 XU Xinyue13 ZHANG Di13 QIAN Gang13 CHEN Ying12*
(1. Co-Innovation Center for the Sustainable Foresity in Southern China, Nanjing Forestry University, Nanjing 210037, China; 2. College of Biology and Environment, Nanjing Forestry University, Nanjing 210037, China; 3. College of Forestry, Nanjing Forestry University, Nanjing 210037, China)
关键词:
落叶冬青 干旱胁迫 茉莉酸甲酯 抗氧化系统 缓解效应
Keywords:
Ilex verticillata L. drought methyl jasmonate antioxidative capacity alleviating effect
分类号:
S725; Q945.78
DOI:
10.3969/ j.issn.1000-2006.201712035
文献标志码:
A
摘要:
【目的】落叶型冬青(Ilex verticillata L.)是一种重要的冬季观果树种,研究其干旱可为扩大其种植范围和提高其适应性提供参考。【方法】以引种落叶冬青扦插苗为试验材料,通过对照、500 μmol/L MJ(茉莉酸甲酯)、干旱、干旱+500 μmol/L MJ(drought+MJ)处理,研究外施MJ、干旱胁迫及复水条件下对落叶冬青叶片光合色素、活性氧、抗氧化水平的影响。【结果】干旱处理第12天时,叶片含水量、叶绿素和类胡萝卜素的含量都显著下降; 而干旱+MJ处理能够显著地提高上述指标的水平。干旱显著地增加了叶片H2O2、O·-2、丙二醛(MDA)的含量,在处理的第12天都分别达到最高点,而添加MJ能够在第8~12天有效降低上述指标的水平。干旱对不同抗氧化酶及抗氧化物产生不同的影响: SOD活性先下降后恢复到对照水平,POD、APX 、GR酶活性及抗氧化物质AsA、GSH含量随处理时间的延长而增加,分别在处理的第8天(GR与GSH)或第12天(POD、APX与AsA)达到高点; 而drought+MJ处理的POD、APX与AsA在第8天、GR与GSH在第3天分别达到高点,以后随着H2O2、MDA和O·-2含量的降低,POD、APX、GR活性和AsA和GSH含量也相应地处于较低水平。干旱复水6 d时落叶冬青苗仍有干旱症状,而drought+MJ处理在复水6 d时各指标基本能恢复到对照水平。【结论】连续12 d的干旱使落叶冬青苗木活性氧的含量显著地增加,造成了氧化胁迫; 添加MJ后能使其抗氧化保护系统的功能迅速开启,清除活性氧,降低氧化胁迫水平,有效缓解干旱胁迫带来的伤害,提高落叶冬青苗的抗旱性。本研究为扩大落叶冬青苗木的种植范围和提高其适应性提供参考。
Abstract:
【Objective】Ilex verticillata L.(winterberry, North America holly)is a deciduous shrub and dioecism, and also an important ornamental and commercial species owing to its red fruits in winter. The study of drought treatment to Ilex verticillata seedlings will provide reference for expanding this species scope of cultivation area.【Method】The effects of methyl jasmonate(MJ)on antioxidative capacity in the leaves by spraying MJ, drought and re-watering to seedlings including control, 500 μmol/L MJ, drought, 500 μmol/L MJ +drought(drought+MJ)treatments were investigated. 【Result】Drought significantly reduced leaf water content,pigment(chlorophyll a, b and carotenoids)content while foliar spray with methyl jasmonate under drought increased all above index levels compared to the control. Drought signifi-cantly increased the contents of H2O2, superoxide radical(O·-2)and malondialdehyde(MDA), and attained to high peak on the 12th day treatment, respectively, while application MJ significantly reduced the levels of these parameters under drought stress. Drought affected antioxidative enzyme activities and antioxidant contents differently: superoxide dismutase(SOD)activity was observed decreasing firstly then increasing, however peroxidase(POD), ascorbate peroxidase(APX), glutathione reductase(GR)activities and AsA or GSH content were increased with the drought time extension, and achieved to high peak at 8th day(GR and GSH)or 12th day(POD, APX and AsA), respectively. On the other hand, application MJ under drought stress, POD, APX activities and AsA content at the 8th day, GR activity and GSH content at the 3th day reached high peaks,respectively. Afterward, these antioxidative enzymes and antioxidants correspondingly restored to the low levels with H2O2, and O·-2 level decreasing. Ilex verticillata seedlings could recover under re-watering for 6 days after Drought+MJ treatment for 12 days, but the opposite result was found under single drought treatment. 【Conclusion】 The application MJ could early and quickly activate the antioxidant protection systems, reduce oxidative stress, relieve the injury of the drought stress and improving drought tolerance of Ilex verticillata seedlings.

参考文献/References:


[1] DIRR M A. Manual of woody landscape plants[M]. 6th ed. Illinois: Stipes Publishing L L C, 2009: 537-541.
[2] GARGIULLO M B, STILES E W. Chemical and nutritional diffe-rences between two bird-dispersed fruits: Ilex opaca and Ilex verticillata[J]. Journal of Chemical Ecology,1991,17(6):1091-1106. DOI:10.1007/BF01402936.
[3] 余有祥. 北美冬青引种栽培[J]. 中国花卉园艺, 2009(10):40-41.
YU Y X. Introduction and cultivation of North American holly[J]. China Flowers & Horticulture, 2009(10):40-41.
[4] 杨玉珍, 陈刚, 王国霞, 等. 北美冬青的园林特性及其应用[J]. 农学学报,2015,5(4):73-76. DOI:10.11923/j.issn.2095-4050.2013-xb0913.
YANG Y Z, CHEN G, WANG G X, et al. Landscape characteristics of North American holly and its application[J]. Journal of Agriculture,2015,5(4):73-76.
[5] DAR T A, UDDIN M, KHAN A M M, et al. Jasmonates counter plant stress: a review[J]. Environmental and Experimental Botany, 2015,115:49-57. DOI:10.1016/j.envexpbot. 2015.02.010.
[6] AHMAD P, RASOOL S, GUL A, et al. Jasmonates: multifunctional roles in stress tolerance[J]. Frontiers in Plant Science, 2016, 7(813):1-15. DOI:10.3389/fpls.2016.00813.
[7] HANAKA A, WóJCIK M, DRESLER S, et al. Does methyl jasmonate modify the oxidative stress response in Phaseolus coccineus treated with Cu?[J]. Ecotoxicology and Environmental Safety, 2016,124:480-488. DOI:10.1016/j.ecoenv.2015.11.024.
[8] JIANG M Q, XU F L, PENG M, et al. Methyl jasmonate regulated diploid and tetraploid black locust(Robinia pseudoacacia L.)tolerance to salt stress[J]. Acta Physiologiae Plantarum, 2016, 38(4):1-13. DOI:10.1007/s11738-016-2120-z.
[9] SALIMI F, SHEKARI F, HAMZEI J. Methyl jasmonate improves salinity resistance in German chamomile(Matricaria chamomilla L.)by increasing activity of antioxidant enzymes[J]. Acta Phy-siologiae Plantarum, 2016,38(1):1-14. DOI:10.1007/s11738-015-2023-4.
[10] 董桃杏, 蔡昆争, 曾任森. 干旱胁迫下茉莉酸甲酯对水稻叶片质膜透性及无机离子含量的影响[J]. 华北农学报, 2010,25(1):136-140. DOI:10.7668/hbnxb.2010.01.027.
DONG T X, CAI K Z, ZENG R S. Effects of methyl jasmonate on membrane permeability and inorganic ions content in rice leaves under drought stress[J]. Acta Agriculturae Boreali-Sinica, 2010,25(1):136-140.
[11] 董桃杏, 蔡昆争, 张景欣, 等. 茉莉酸甲酯(MeJA)对水稻幼苗的抗旱生理效应[J]. 生态环境, 2007,16(4):1261-1265. DOI:10.16258/j.cnki.1674-5906.2007.04.042.
DONG T X, CAI K Z, ZHANG J X, et al. The physiological roles of methyl jasmonate(MeJA)in drought resistance of rice seedlings[J]. Ecology and Environment, 2007,16(4):1261-1265.
[12] 董桃杏, 蔡昆争, 曾任森. 茉莉酸甲酯(MeJA)对干旱胁迫下水稻幼苗光合作用特性的影响[J]. 生态环境学报, 2009,18(5):1872-1876. DOI:10.16258/j.cnki.1674-5906.2009.05.066.
DONG T X, CAI K Z, ZENG R S. Effects of methyl jasmonate(MeJA)on photosynthetic traits of rice seedlings under drought stress[J]. Ecology and Environmental Sciences, 2009, 18(5): 1872-1876.
[13] 潘瑞炽, 古焕庆. 茉莉酸甲酯对花生幼苗生长和抗旱性的影响[J].生理学报,1995,21(3): 215-220.
PAN R C, GU H Q. Effect of methyl jasmonate on the growth and drought resistance in peanut seedlings[J]. Acta Physiologica Sinica,1995,21(3):215-220.
[14] de OLLAS C, HERNANDO B, ARBONA V, et al. Jasmonic acid transient accumulation is needed for abscisic acid increase in citrus roots under drought stress conditions[J]. Physiologia Plantarum, 2013,147(3):296-306. DOI:10.1111/j.1399-3054.2012.01659.x.
[15] 陈茜, 周之涵, 王瑞琪, 等. 低温处理对北美冬青叶片抗氧化能力的影响[J]. 热带亚热带植物学报, 2016, 24(6):689-695. DOI:10.11926/j.issn.1005-3395.2016.06.013.
CHEN X, ZHOU Z H, WANG R Q, et al. Effect of low temperature stress on antioxidant ability in Ilex verticillata Leaves[J]. Journal of Tropical and Subtropical Botany, 2016, 24(6):689-695.
[16] CHEN Y, LUO Y Y, QIU N F, et al. Ce3+ induces flavonoids accumulation by regulation of pigments, ions, chlorophyll fluore-scence and antioxidant enzymes in suspension cells of Ginkgo biloba L.[J]. Plant Cell, Tissue and Organ Culture,2015,123(2):283-296. DOI:10.1007/s11240-015-0831-2.
[17] 陈建勋, 王晓峰. 植物生理学实验指导[M].2版.广州:华南理工大学出版社, 2007.
CHEN J X, WANG X F. Plant physiology experiment guide[M]. 2nd ed. Guangzhou: South China University of Technology Press, 2007.
[18] 杨艺, 常丹, 王艳, 等. 茉莉酸甲酯对棉花抗旱效果的影响[J]. 西北农业学报,2016,25(9): 1333-1341. DOI:10.7606/j.issn.1004-1389.2016.09.009.
YANG Y, CHANG D, WANG Y, et al. Effect of methyl jasmonate(MeJA)on enhancing drought resistance of cotton[J]. Acta Agriculturae Boreali-occidentalis Sinica,2016,25(9): 1333-1341.
[19] MAHMOOD M, BIDABADI S S, GHOBADI C, et al. Effect of methyl jasmonate treatments on alleviation of polyethylene glycol-mediated water stress in banana(Musa acuminata cv. ‘Berangan’, AAA)shoot tip cultures[J]. Plant Growth Regulation,2012,68(2):161-169. DOI:10.1007/s10725-012-9702-6.
[20] ANJUM S A, XIE X Y, FAROOQ M, et al. Effect of exogenous methyl jasmonate on growth, gas exchange and chlorophyll contents of soybean subjected to drought[J]. African Journal of Biotechnology,2011,10(47):9640-9646. DOI:10.5897/AJB10.2641.
[21] MILLER G, SUZUKI N, CIFTCI-YILMAZ S, et al. Reactive oxygen species homeostasis and signalling during drought and salinity stresses[J]. Plant, Cell & Environment,2010,33(4):453-467. DOI:10.1111/j.1365-3040.2009.02041.x.
[22] LIU X, CHI H, YUE M, et al. The regulation of exogenous jasmonic acid on UV-B stress tolerance in wheat[J]. Journal of Plant Growth Regulation,2012,31(3):436-447. DOI:10.1007/s00344-011-9253-5.
[23] ALAM M M, NAHAR K, HASANUZZAMAN M, et al. Exogenous jasmonic acid modulates the physiology, antioxidant defense and glyoxalase systems in imparting drought stress tolerance in different Brassica species[J]. Plant Biotechnology Reports,2014,8(3):279-293. DOI:10.1007/s11816-014-0321-8.
[24] SIBOZA X I, BERTLING I, ODINDO A O. Enzymatic antioxidants in response to methyl jasmonate and salicylic acid and their effect on chilling tolerance in lemon fruit [Citrus Limon(L.)Burm. F.][J]. Scientia Horticulturae,2017,225:659-667. DOI:10.1016/j.scienta.2017.07.023.
[25] BIDESHKI A, ARVIN M J. Interactive effects of methyl jasmonate(MJ)and indole-3 butyric acid(IBA)on growth and bio chemical parameters, bulb and allicin yield of garlic(Allium sativum L.)under drought stress in Iran[J]. International Journal of Agriculture: Research and Review,2013,3(2): 349-360.
[26] POLJSAK B, UPUT D, MILISAV I. Achieving the balance between ROS and antioxidants: when to use the synthetic antioxidan-ts[J]. Oxidative Medicine and Cellular Longevity,2013:1-11. DOI:10.1155/2013/956792.
[27] 安玉艳, 梁宗锁. 植物应对干旱胁迫的阶段性策略[J]. 应用生态学报,2012,23(10):2907-2915. DOI:10.13287/j.1001-9332.2012.0403.
AN Y Y, LIANG Z S. Staged strategy of plants in response to drought stress[J]. Chinese Journal of Applied Ecology,2012,23(10):2907-2915.
[28] SAMOTA M K, SASI M, AWANA M, et al. Elicitor-induced biochemical and molecular manifestations to improve drought tolerance in rice(Oryza sativa L.)through seed-priming[J]. Frontiers in Plant Science,2017,8(934):1-13. DOI:10.3389/fpls.2017.00934.
[29] VATANPARAST G, MIRDEHGHAN S H, KARIMI H R, et al. Foliar application of salicylic acid, methyl jasmonate and potassium sulfate on photosynthetic characteristics and fruit quality of pomegranate[J]. Iran Agricultural Research,2013,31(2):23-34. DOI:10.22099/IAR.2013.1524.
[30] 金微微, 王炎, 张会慧, 等. 茉莉酸甲酯对干旱及复水条件下烤烟幼苗叶绿素荧光特性的影响[J]. 应用生态学报,2011,22(12):3157-3162. DOI:10.13287/j.1001-9332.2011.0441.
JIN W W, WANG Y, ZHANG H H, et al. Effects of foliar spraying methyl jasmonate on leaf chlorophyll fluorescence characte-ristics of flue-cured tobacco seedlings under drought and re-watering[J]. Chinese Journal of Applied Ecology,2011,22(12):3157-3162.

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备注/Memo

备注/Memo:
收稿日期:2017-12-21 修回日期:2018-07-10
基金项目:国家林业局“948”项目(2014-4-61); 江苏高校优势学科建设工程资助项目(PAPD); 南京林业大学大学生创新训练计划项目(2016NFUSPITP002)
第一作者:陈燕琼(249589249@qq.com),负责结果分析与论文撰写。沈(2276582506@qq.com),负责数据分析与部分论文撰写。*通信作者:陈颖(chynjfu@163.com),教授。
更新日期/Last Update: 2018-11-30