[1]孙岩啸,王贤荣*,孙 蕾,等.无花果栽培品种遗传多样性分析及SSR指纹图谱构建[J].南京林业大学学报(自然科学版),2018,42(06):197-202.[doi:10.3969/j.issn.1000-2006.201612028]
 SUN Yanxiao,WANG Xianrong*,SUN Lei,et al.Genetic diversity analysis and fingerprint construction of fig(Ficus carica Linn.)cultivars by SSR markers[J].Journal of Nanjing Forestry University(Natural Science Edition),2018,42(06):197-202.[doi:10.3969/j.issn.1000-2006.201612028]
点击复制

无花果栽培品种遗传多样性分析及SSR指纹图谱构建
分享到:

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

卷:
42
期数:
2018年06期
页码:
197-202
栏目:
研究简报
出版日期:
2018-12-15

文章信息/Info

Title:
Genetic diversity analysis and fingerprint construction of fig(Ficus carica Linn.)cultivars by SSR markers
作者:
孙岩啸1王贤荣1*孙 蕾2贾 明2
(1.南京林业大学生物与环境学院, 江苏 南京 210037; 2.山东省林业科学研究院,山东 济南 250014)
Author(s):
SUN Yanxiao1 WANG Xianrong1* SUN Lei2 JIA Ming2
(1. College of Biological and Environmental, Nanjing Forestry University, Nanjing 210037, China; 2. Shandong Academy of Forestry,Ji’nan 250014, China)
关键词:
无花果 SSR 指纹图谱 遗传多样性
Keywords:
Ficus carica Linn.(fig) simple sequence repeat(SSR) fingerprint genetic diversity
分类号:
S663.3
DOI:
10.3969/j.issn.1000-2006.201612028
文献标志码:
A
摘要:
【目的】近几年国内引进了很多的无花果新品种,但是仅依靠表型性状不能准确地区分相似品种,因此从DNA分子水平上进行无花果品种的分类和鉴定。【方法】利用SSR分子标记技术对30个无花果品种进行遗传多样性分析和DNA指纹图谱构建。【结果】7对SSR引物在30个无花果品种中共扩增得到了24条清晰条带,平均观察等位基因数(Na)为3.43、有效等位基因数(Ne)为3.025 6、Shannon信息指数(I)为1.126 6,多态性信息含量指数(PIC)变化从0.682 8到0.892 5,平均为0.816 4。遗传相似系数在0.291 7~1.000 0之间,平均相似系数为0.629 4,表明实验的品种间存在差异。在相似系数为0.59时将30个无花果品种分为4类。同时构建了30个无花果品种的SSR指纹图谱。【结论】基于SSR分子标记技术建立起来的无花果指纹图谱能够有效区分无花果相似品种,可以为无花果新品种的选育和品种鉴定提供科学依据。
Abstract:
【Objective】In recent years, with numbers of new fig(Ficus caria Linn.)cultivars have been introduced in China, it is necessary to classify and identify fig cultivars from the DNA molecular level. 【Method】We analyzed the genetic diversity and constructed DNA fingerprint of 30 fig cultivars by simple sequence repeat(SSR)molecular marker technology, which provided the scientific basis for the classification and identification of fig cultivars.【Results】The 24 fragments were generated from 7 pairs of SSR primers. The average of Na, Ne, and Shannon information index(I)were 3.43, 3.025 6, and 1.126 6, respectively. The polymorphic information content(PIC)values ranged from 0.682 8 to 0.892 5, with a mean of 0.816 4. The genetic similarity coefficient ranged from 0.291 7 to 1.000 0, and the average similarity coefficient was 0.629 4, indicating differences between the 30 fig cultivars. And thirty fig cultivars were divided into four categories when the genetic similarity coefficient was 0.59.【Conclusion】This fingerprinting experiment based on the SSR molecular markers distinguished similar cultivars and provided scientific basis for breeding and identification of new fig cultivars.

参考文献/References:


[1] BAROLO M I, MOSTACERO N R, LOPEZ S N. Ficus carica L.(Moraceae): an ancient source of food and health [J]. Food Chemistry, 2014, 164(1):119-127.DOI:10.1016/j.foodchem.2014.04.112.
[2] MAWA S,HUSAIN K,JANTAN I.Ficus carica L.(Moraceae ):phytochemistry,traditional uses and biological activities[J].Evidence-Based Complementary and Alternative Medicine, 2013:1-8. DOI:10.1155/2013/974256.
[3] 李新军,黄敏仁,潘惠新,等.林木基因组中的微卫星(SSR)及其应用[J]. 南京林业大学学报,1999,23(5):64-69.DOI:10.3969/j.issn.1000-2006.1999.05.010.
LI X J, HUANG M R, PAN H X, et al.Microsatellite markers and appication in the forestry genome[J]. Journal of Nanjing Forestry University, 1999, 23(5): 64-69.
[4] 樊蓉,樊军锋,李周岐. 9个白杨品种SSR指纹图谱构建及遗传关系的研究[J].西北林学院学报,2015,30(3):76-80. DOI:10.3969/j.issn.1001-7461.2015.03.14.
FAN R, FAN J F, LI Z Q. Fingerprinting and genetic relatedness of 9 varieties in Populus L. Sect. Populus using SSR markers[J]. Journal of Northwest Forestry University, 2015,30(3):76-80..
[5] 段一凡,王贤荣,梁丽丽,等.桂花品种SSR荧光指纹图谱的构建[J]. 南京林业大学学报(自然科学版),2014,38(S1):1-6. DOI:10.3969/j.issn.1000-2006.2014.S1.001.
DUAN Y F, WANG X R, LIANG L L,et al. Fingerprinting and identification of Osmanthus fragrans cultivars using fluorescence-labeled SSR markers[J]. Journal of Nanjing Forestry University(Natural Sciences Edition), 2014, 38(S1): 1-6.
[6] 乔中全, 王晓明, 李永欣,等. 桂花优良品种‘珍珠彩桂’遗传多样性的ISSR分析及指纹图谱构建[J]. 湖南林业科技,2016,43(3):1-5. DOI:10.3969/j.issn.1003-5710.2016.03.001.
QIAO Z Q, WANG X M, LI Y X, et al. Genetic diversity and fingerprint construction of varieties of Osmanthus fragrans‘Zhenzhu Caigui’by ISSR markers[J]. Hunan Forestry Science & Technology,2016,43(3):1-5.
[7] 王亮,王彩虹,田义轲,等. 无花果品种的RAPD及SSR指纹分析[J]. 青岛农业大学学报(自然科学版), 2009,26(4):297-301. DOI:10.3969/j.issn.1674-148X.2009.04.008.
WANG L, WANG C H, TIAN Y K, et al. DNA fingerprints for fig(Ficus carica)varieties identification by RAPD and SSR ana-lysis[J].Journal of Qingdao Agricultural University(Natural Science),2009,26(4):297-301.
[8] 王清明,叶宇芸,马建伟,等. 成都平原桃主栽品种EST-SSR指纹图谱构建[J]. 分子植物育种,2016,14(3):638-646.DOI:10.13271/j.inpb.014.000638.
WANG Q M, YE Y Y, MA J W, et al. Establishment of EST-SSR fingerprint for the major cultivars of peach in Chengdu Plain[J]. Molecular Plant Breeding, 2016, 14(3): 638-646.
[9] 高雄梅,徐国辉,王贺新,等. 基于EST-SSR标记的蓝莓品种指纹图谱构建及遗传多样性[J].贵州农业科学,2016,44(4):110-115. DOI:10.3969/j.issn.1001-3601.2016.04.028.
GAO X M, XU G H, WANG H X, et al Fingerprinting construction and genetic relationship analysis of blueberry bases on EST-SSR markers[J]. Guizhou Agricultural Sciences, 2016, 44(4): 110-115.
[10] 于卓,李岩,于肖夏,等. 马铃薯杂种优良株系产量品质及SSR指纹差异分析[J].种子,2016,35(5):23-26. DOI:10.16590/j.cnki.1001-4705.2016.05.023.
YU Z, LI Y, YU X X, et al. Difference analysis of yield, quality and SSR fingerprint of excellent potato hybrid lines[J]. Seed, 2016, 35(5): 23-26.
[11] 吴则东.甜菜栽培品种的DNA指纹图谱构建及遗传多样性分析[D].北京:中国农业科学院,2013.
WU Z D. Establishment of DNA fingerprint map and analysis of genetic diversity among beet cultivar [D]. Beijing: Chinese Academy of Agricultural Sciences Dissertation, 2013.
[12] 田义轲,王彩虹,张继澍,等.一个与苹果柱型基因(Co)连锁的RAPD标记[J].西北植物学报,2003,23(12):2176-2179. DOI:10.3321/j.issn:1000-4025.2003.12.023.
TIAN Y K, WANG C H, ZHANG J S, et al. A RAPD marker of apple columnar gene(Co )[J]. Acta Bot Boreal-Occident Sin, 2003,23(12): 2176-2179.
[13] KHADARI B, HOCHU I, SANTONI S, et al. Identification and characterization of microsatellite loci in the common fig(Ficus carica L.)and representative species of genus Ficus[J]. Mol Ecol Notes, 2001(1):191-193. DOI:10.1046/j.1471-8278.2001.00072.x.
[14] GIRALDO E, VIRUEL M A, LOPEZ-C ORRALES M, et al. Characterisation and cross-species transferability of microsatellites in the common fig(Ficus carica L.)[J]. Hort Sci Biotech, 2005, 80: 217-24. DOI:10.1080/14620316.2005.11511920.
[15] BANDELJ D,JAVRNIK B, JAKSE J. Development of microsatellite markers in the common fig, Ficus carica[J].Molecular Ecology Notes, 2007(7): 1311-1314. DOI:10.1111/j.1471-8286.2007.01866.x.
[16] NEI M, LI W H. Mathematical models for studying genetic variation in terms of restriction endonucléases [J]. Proc Natl Acad Sci USA, 1979,76:5269-5273. DOI:10.1073/pnas.76.10.5269.
[17] YEH F C, YANG R C, BOYLE T. Popgene version 1.31 quick user guide[R]. Canada: University of Alberta, and Center for Intermatiomal Forestry Research, 1999.
[18] ROHLF F J. NTSYS-pc, numerical taxonomy and multivariate analysis system[EB/OL].(1994)[2017]. http:// doc.docson.com/bbc3ef041af852d3e71aad7b1.html.
[19] 王静毅,陈业渊,黄秉智,等.部分香蕉品种SSR指纹图谱的构建[J].果树学报,2009,26(5):733-738. DOI:10.13925/j.cnki.gsxb.2009.05.010.
WANG J Y, CHEN Y Y, HUANG B Z, et al. Establishment of fingerprinting for bananas(Musa nana)by SSR marker[J]. Journal of Fruit Science, 2009, 26(5): 733-738.
[20] 宋海斌,崔喜波,马鸿艳,等.基于SSR标记的甜瓜品种(系)DNA指纹图谱库的构建[J].中国农业科学,2012,45(13):2676-2689. DOI:10.3864/j.issn.0578-1752.2012.13.012.
SONG H B, CUI X B, MA H Y,et al.Construction of DNA fingerprint database based on SSR marker for varieties(Lines)of Cucumis melo L.[J].Scientia Agricultura Sinica, 2012,45(13):2676-2689.

相似文献/References:

[1]李世峰,张博,陈英,等.美洲黑杨种质资源遗传多样性的SSR分析[J].南京林业大学学报(自然科学版),2006,30(04):010.[doi:10.3969/j.jssn.1000-2006.2006.04.003]
 LI Shi-feng,ZHANG Bo,CHEN Ying,et al.Analysis of Genetic Diversity of Populus deltoides Germplasm by SSR[J].Journal of Nanjing Forestry University(Natural Science Edition),2006,30(06):010.[doi:10.3969/j.jssn.1000-2006.2006.04.003]
[2]胡建刚,郭继善.无花果的组织培养[J].南京林业大学学报(自然科学版),1994,18(03):073.[doi:10.3969/j.jssn.1000-2006.1994.03.015]
 Hu Jiangang,Guo Jishan(Nangjing Forestry School Nanjing 00).A STUDY ON TISSUE CULTURE OF FICUS CARICA[J].Journal of Nanjing Forestry University(Natural Science Edition),1994,18(06):073.[doi:10.3969/j.jssn.1000-2006.1994.03.015]
[3]徐 阳,陈金慧,赵亚琦,等.杉木地理种源的EST-SSR分子标记变异研究[J].南京林业大学学报(自然科学版),2014,38(01):001.[doi:10.3969/j.issn.1000-2006.2014.01.001]
 XU Yang,CHEN Jinhui,ZHAO Yaqi,et al.Variation of EST-SSR molecular markers among provenances of Chinese fir[J].Journal of Nanjing Forestry University(Natural Science Edition),2014,38(06):001.[doi:10.3969/j.issn.1000-2006.2014.01.001]
[4]马秋月,廖卓毅,张得芳,等.碧桃花瓣转录组微卫星特征分析[J].南京林业大学学报(自然科学版),2015,39(03):034.[doi:10.3969/j.issn.1000-2006.2015.03.007]
 MA Qiuyue,LIAO Zhuoyi,ZHANG Defang,et al.Deep sequenced-based transcriptome analysis of microsatellites in peach(Prunus persica cv. duplex) flowers[J].Journal of Nanjing Forestry University(Natural Science Edition),2015,39(06):034.[doi:10.3969/j.issn.1000-2006.2015.03.007]
[5]冯源恒,杨章旗*,李火根*,等.不同时期广西马尾松优良种源的遗传多样性变化趋势[J].南京林业大学学报(自然科学版),2016,40(05):041.[doi:10.3969/j.issn.1000-2006.2016.05.007]
 FENG Yuanheng,YANG Zhangqi*,LI Huogen*,et al.A study on changes of genetic diversity for nearly 50 years in superior provenances of Pinus massoniana in Guangxi[J].Journal of Nanjing Forestry University(Natural Science Edition),2016,40(06):041.[doi:10.3969/j.issn.1000-2006.2016.05.007]
[6]伊贤贵,陈 洁,尤禄祥,等.山樱花群体遗传多样性的SSR分析[J].南京林业大学学报(自然科学版),2018,42(05):025.[doi:10.3969/j.issn.1000-2006.201702036]
 YI Xiangui,CHEN Jie,YOU Luxiang,et al.Genetic divetsity of Cerasus serrulata populations assessed by SSR markers[J].Journal of Nanjing Forestry University(Natural Science Edition),2018,42(06):025.[doi:10.3969/j.issn.1000-2006.201702036]
[7]冯源恒,杨章旗*,李火根,等.马尾松育种进程中的遗传增益与遗传多样性变化[J].南京林业大学学报(自然科学版),2018,42(05):196.[doi:10.3969/j.issn.1000-2006.201706022]
 FENG Yuanheng,YANG Zhangqi *,LI Huogen,et al.Changes of genetic gain & genetic diversity in the breeding process of Pinus massoniana[J].Journal of Nanjing Forestry University(Natural Science Edition),2018,42(06):196.[doi:10.3969/j.issn.1000-2006.201706022]

备注/Memo

备注/Memo:
收稿日期:2016-12-15 修回日期:2018-09-16
基金项目:国家林业公益性行业重大科研专项项目(201204402)
第一作者:孙岩啸(285907417@qq.com),博士生。*通信作者:王贤荣(106223175@qq.com),教授。
更新日期/Last Update: 2018-11-30