[1]刘 玲,王 丹*,黎熠睿,等.60Co-γ射线对小苍兰的生物学效应[J].南京林业大学学报(自然科学版),2019,43(01):186-192.[doi:10.3969/j.issn.1000-2006.201801024]
 LIU Ling,WANG Dan*,LI Yirui,et al.The biological effects of 60Co-γ rays on Freesia refracta[J].Journal of Nanjing Forestry University(Natural Science Edition),2019,43(01):186-192.[doi:10.3969/j.issn.1000-2006.201801024]
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60Co-γ射线对小苍兰的生物学效应
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《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

卷:
43
期数:
2019年01期
页码:
186-192
栏目:
研究简报
出版日期:
2019-01-28

文章信息/Info

Title:
The biological effects of 60Co-γ rays on Freesia refracta
文章编号:
1000-2006(2019)01-0186-07
作者:
刘 玲1王 丹1*黎熠睿1闵可怜1陈 敏1张继文1邓善文1黎 青2向 毅2李建伟2
1.西南科技大学生命科学与工程学院,四川 绵阳 621010; 2.四川省农业科学院生物技术核技术研究所,四川 成都 610011
Author(s):
LIU Ling1 WANG Dan1* LI Yirui1 MIN Kelian1 CHEN Min1 ZHANG Jiwen1 DENG Shanwen1LI Qing2XIANG Yi2LI Jianwei2
1.College of Life Science and Engineering,Southwest University of Science and Technology,Mianyang 621010,China; 2. Biotechnology and Nuclear Technology Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610011,China
关键词:
小苍兰 60Co-γ射线 辐射诱变育种 种球
Keywords:
Freesia refracta Klatt 60Co-γ rays irradiation breeding bulbs
分类号:
S688.2;S722.3
DOI:
10.3969/j.issn.1000-2006.201801024
文献标志码:
A
摘要:
【目的】考察60Co-γ射线辐照对红色小苍兰和紫色小苍兰的生物学效应。【方法】以试验剂量分别为25、50、75、100 Gy的60Co-γ射线辐照两个不同品种的小苍兰种球,统计分析其受辐照后的发芽率、半致死剂量、成活率、株高、叶片数量、叶片面积、根长、开花率、花粉活力、花粉电镜扫描等指标。【结果】经5个不同剂量60Co-γ射线处理的小苍兰两个品种的发芽率、株高、叶面积、根长、根数、开花率、花粉活力均随着剂量的增加而降低。小苍兰(红色)的最适宜辐照剂量为54.04 Gy,小苍兰(紫色)的适宜辐照剂量为58.82 Gy; 小苍兰(红色)的半开花辐照剂量为42.37 Gy,小苍兰(紫色)半开花辐照剂量为39.56 Gy。随着辐照剂量的增加花粉表面和花粉形态均受损程度逐渐增强。【结论】不同剂量的60Co-γ射线辐照对小苍兰两个品种均有损伤效应,损伤程度与辐照剂量成正相关; 对花粉的分析能够较好地反映辐照对植株的诱变情况。
Abstract:
【Objective】 Investigate the biological effects of 60Co-γ radiation on red Freesia refracta(freesia)and purple freesia. 【Method】 In this study, two different varieties of freesia bulbs were irradiated with 60Co-γ rays at test doses of 25, 50, 75, and 100 Gy. Statistical analyses of the germination rate, semilethal dose, survival rate, plant height, leaf number, leaf area, root length, flowering rate, pollen viability, and pollen scanning electron microscope after irradiation were conducted. 【Result】 The experimental results demonstrate that the germination rate, plant height, leaf area, root length, flowering rate and pollen vigor of the two freesias treated with five different doses of 60Co-γ rays decrease with increasing doses. The optimum dose of freesia(red)is 54.04 Gy, the optimum dose of freesia(purple)is 58.82 Gy, the half flowering dose of freesia(red)is 42.37 Gy, the half flowering dose of freesia(purple)is 39.56 Gy. Pollen surface and pollen morphology gradually increase with increasing irradiation. 【Conclusion】60Co-γ rays of various irradiation doses have damage effects on both freesias, and the degree of damage is positively correlated to the radiation dosage. Analyses on pollen can better reflect the plant mutation induced by radiation of the 60Co-γ rays. The results can serve as references for the mutation breeding of freesia induced by 60Co-γ rays radiation.

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备注/Memo

备注/Memo:
收稿日期:2018-01-25 修回日期:2018-05-10基金项目:四川省辐射诱变技术育种平台资助项目(2016NZ0106)。 第一作者:刘玲(497104095@qq.com)。*通信作者:王丹(wangdan@swust.edu.cn),教授,ORCID(0000-0002-9289-5563)。引文格式:刘玲,王 丹,黎熠睿,等. 60Co-γ射线对小苍兰的生物学效应[J]. 南京林业大学学报(自然科学版),2019,43(1):186-192.
更新日期/Last Update: 2019-01-28