'杨' 附近有语法错误。 '杨' 附近有语法错误。 字符串 ' order by num ' 后的引号不完整。 杨树雄性不育品种花粉发育过程的细胞学观察-《南京林业大学学报(自然科学版)》


点击复制

杨树雄性不育品种花粉发育过程的细胞学观察
分享到:

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

卷:
43
期数:
2019年01期
页码:
198-203
栏目:
研究简报
出版日期:
2019-01-28

文章信息/Info

Title:
Cytological observation on pollen development of the male sterile poplar variety
文章编号:
1000-2006(2019)01-0198-05
作者:
刘望舒1韩林芝2朱 嵊3潘惠新1*
1.南京林业大学林学院, 江苏 南京 210037; 2. 江苏润扬大桥发展有限责任公司,江苏 镇江 212100; 3.南京林业大学生物与环境学院,江苏 南京 210037
Author(s):
LIU Wangshu1HAN Linzhi2ZHU Cheng3PAN Huixin1*
1.College of Forestry, Nanjing Forestry University, Nanjing 210037, China; 2.Jiangsu Runyang Bridge Development Co., Ltd., Zhenjiang 212100, China; 3. College of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, China
关键词:
Keywords:
分类号:
S722;Q23
DOI:
10.3969/j.issn.1000-2006.201805034
文献标志码:
A
摘要:
【目的】了解杨树小孢子败育的时期及影响小孢子败育的因素,为杨树雄性不育新品种选育提供科学依据。【方法】以杨树雄性不育品种‘泗杨1号'为材料,在光学显微镜下观察花粉粒细胞发育过程; 在扫描电子显微镜下进行败育花粉粒的形态观察,并与可育品种‘南林3412'杨进行比较分析。【结果】显微镜下观测发现在四分体时期以后,即单核小孢子期,雄性不育品种‘泗杨1号'大部分小孢子发生异常,开始表现出败育性状。在花粉粒成熟期小孢子开始发生解体,小孢子败育。同时,用扫描电子显微镜观察仅有的少量花粉粒发现其异常变形,不同于正常花粉粒形态。【结论】‘泗杨1号'小孢子败育从单核小孢子时期开始,在花粉粒成熟期小孢子发生解体,小孢子败育。影响‘泗杨1号'小孢子败育主要原因是绒毡层细胞降解的延迟,影响小孢子发育营养物质的供给,导致小孢子发生败育; 除此之外,花药室内的Ca2+浓度、花药内壁乌氏体的分布也有可能影响‘泗杨1号'小孢子的发育过程。
Abstract:
【Objective】 Understand the period of microspore abortion and the factors affecting microspore abortion in poplar varieties to provide the scientific basis for breeding new male sterile poplar varieties.【Method】This paper used the male sterile Poplulus×euramericana ‘Siyang-1' aged 16 years as study material. In spring 2016, the flower branches were collected with water training at room temperature. Then, we regularly sampled and fixed the material to make paraffin sections, and observe the cellular development of pollen grains under an Olympus-bx51 optical microscope. Moreover, we observed the morphology of abortive pollen grains under a Quanta 200 scanning electron microscope and compared it with that of the male fertile variety ‘Nanlin 3412.' 【Result】The microspore development of ‘Siyang-1' began to develop abnormality after the tetrad period. In the mononuclear microspore phase, only a few cells formed mononuclear microspores, which were in a highly vacuolated or disintegrated state. The volume of microspores was obviously increased, showing signs of abortion. At the mature stage of pollen grains, the microspores began to disintegrate and the microspores were aborted. Only a small number of cells developed into two-celled pollen grains bonded to the remaining tapetum, which accumulated and clung to the inner wall of the anthers. The anthers shrank and dried, and no pollen was scattered. At the same time, the pollen grain morphology of ‘Siyang-1' was observed with the Quanta 200 scanning electron microscope. It was found that the pollen grains were abnormal and deformed, which was different from that of the normal pollen grains.【Conclusion】The abortion of ‘Siyang-1' began in the mononuclear microspore stage, and disintegrated at the mature stage of pollen grains, resulting in microspore abortion. The main reason affecting the abortion of ‘Siyang-1' was the delayed degradation of tapetum cells, which influenced the supply of nutrients to microspore development, resulting in microspore abortion. In addition, the concentration of Ca2+ in the anther chamber and the distribution of Uribe in the anther wall may also affect the process of microspore development. This paper provides a new idea for the study of male sterility of poplars, which is beneficial in the production practice in the future.

参考文献/References:

相似文献/References:

备注/Memo

备注/Memo:
更新日期/Last Update: 2019-01-28