PAN Yanyan,XU Guiyou,DONG Lihu,et al.Genetic variations of seedling growth traits among full-sib families of Larix kaempferi[J].Journal of Nanjing Forestry University(Natural Science Edition),2019,43(02):014-22.[doi:10.3969/j.issn.1000-2006.201803038]





Genetic variations of seedling growth traits among full-sib families of Larix kaempferi
(1. 东北林业大学,林木遗传育种国家重点实验室,黑龙江 哈尔滨 150040; 2. 吉林省柳河县五道沟林场,吉林 通化 135300; 3. 吉林省林业科学研究院,吉林 长春 130018)
PAN Yanyan13 XU Guiyou2 DONG Lihu1 WANG Chenglu2LIANG Deyang1ZHAO Xiyang1*
(1. State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, China; 2. Wudaogou Forestry Center of Liuhe County,Tonghua 135300, China; 3. Jilin Provincial Academy of Forestry Sciences, Changchun 130018, China)
日本落叶松 全同胞家系 遗传变异 生长性状 综合评价
Larix kaempferi full-sib family genetic variation growth trait comprehensive assessment
【目的】研究日本落叶松全同胞家系苗期生长性状遗传变异,为日本落叶松遗传改良提供可靠依据。【方法】以67个日本落叶松全同胞家系树高和地径为材料,对树高和地径进行方差分析、遗传参数分析、相关性分析、配合力分析以及家系选择。【结果】方差分析表明:各变异来源差异均达极显著水平(P<0.01); 树高和地径的表型变异系数分别为30.23%和21.51%,遗传变异系数分别为15.98%和16.99%。树高和地径的家系遗传力均为0.94,单株遗传力均为0.52; 通过一般配合力计算初选三棚04为优良父本,三棚37为优良母本,通过特殊配合力计算,东丰18 × 兰山25、东丰81 × 兰山25、三棚98 × 三棚74和临江34 × 兰山28杂交组合为优良组合。以树高和地径为评价指标,以10%的入选率进行优良家系选择,入选的7个家系树高和地径的平均值分别为0.70 m和1.06 cm,遗传增益分别为35.02%和29.01%。以2%的入选率进行优良单株选择,入选的50个单株树高和地径的平均值分别为0.98 m和1.35 cm,遗传增益分别为47.92%和34.67%。【结论】选出的7个优良家系和50个优良单株,可作为建立改良种子园和二代种子园的育种材料。
【Objective】Determine the potential for Larix kaempferi full-sib families to provide the basis for genetic improvement by evaluating their genetic variation and selection potential.【Method】Sixty-seven full-sib families of L. kaempferi were taken as source materials to investigate the tree height and diameter at breast height. The ANOVA, genetic parameter, correlation and combining ability analyses plus family selection were carried out for the tree height and diameter at breast height. 【Result】The result of the ANOVA showed that there were significant differences among all variance sources(P<0.01). The phenotypic variation coefficients of tree height and diameter at breast height were 30.23% and 21.51%,respectively; while the genetic variation coefficients were 15.98% and 16.99%, respectively. The family-level heritability of tree height and diameter at breast height were 0.94, while the individual plant heritability of tree height and diameter at breast height were 0.52, respectively. According to the calculation of the general combining ability, Sanpeng 04 was selected as an excellent male parent, while Sanpeng 37 was selected as an excellent female parent. According to the calculated special combining ability, Dongfeng 18 × Lanshan 25, Dongfeng 81 × Lanshan 25, Sanpeng 98 × Sanpeng 74, and Linjiang 34 × Lanshan 28 were selected as elite hybrids combinations. Using a 10% selection rate, seven families were selected as elite families, with the average of tree height and diameter at breast height of 0.70 m and 1.06 cm and genetic gains of 35.02% and 29.01%, respectively. However, with a 2% selection rate, 50 individual plants were selected as elite individual plants, with an average tree height and diameter at breast height of 0.98 m and 1.35 cm, and genetic gains of 47.92% and 34.67%, respectively. 【Conclusion】Seven elite families and 50 individual plants can provide superior materials for the establishment of improved initial and second generation seed orchards.


[1] ZHOU G X, HU X, ZHU Q, et al. Research on the relationship between stand productivity and site condition of Larix kaempferi [J]. Ecological Economy, 2008, 4(2): 216-226.
[2] 潘志刚,游应天. 中国主要外来树种引种栽培[M]. 北京:北京科学技术出版社,1994:91-99.
PAN Z G, YOU Y T. Introduce and cultivate of the main exotic tree species in China [M]. Beijing: Science and Technology Press,1994:91-99.
[3] FUKATSU E, TSUBOMURA M, FUJISAWA Y, et al. Genetic improvement of wood density and radial growth in Larix kaempferi results from a diallel mating test[J]. Annals of Forest Science, 2013, 70(5): 451-459. DOI:10.1007/s13595-013-0278-8.
[4] 董健, 尤文忠, 黄国学, 等. 日本落叶松良种选育的现状及发展对策[J]. 辽宁林业科技, 2003(6): 27-29. DOI:10.3969/j.issn.1001-1714.2003.06.013.
DONG J, YOU W Z, HUANG G X, et al. Status and development countermeasures of fine varieties selection and breeding of Larix kaempferi[J]. Liaoning Forestry Science and Technology, 2003(6): 27-29.
[5] NAGAMITSU T, NAGASAKA K, YOSHIMARU H, et al. Provenance tests for survival and growth of 50-year-old Japanese larch(Larix kaempferi)trees related to climatic conditions in central Japan[J]. Tree Genetics & Genomes, 2014, 10(1): 87-99. DOI:10.1007/s11295-013-0666-0.
[6] DIAO S, HOU Y M, XIE Y H, et al. Age trends of genetic parameters, early selection and family by site interactions for growth traits in Larix kaempferi open-pollinated families[J]. BMC Genetics, 2016, 17: 104. DOI:10.1186/s12863-016-0400-7.
[7] 杜超群, 许业洲, 孙晓梅, 等. 鄂西亚高山区日本落叶松无性系生长性状变异分析与早期选择[J]. 华中农业大学学报, 2015, 34(3): 19-23. DOI:10.13300/j.cnki.hnlkxb.2015.03.020.
DU C Q, XU Y Z, SUN X M, et al. Variation of growth traits and early selection of Larix kaempferi clones in sub-alpine area of western Hubei Province[J]. Journal of Huazhong Agricultural University, 2015, 34(3): 19-23.
[8] NAKADA R, FUJISAWA Y, TANIGUCHI T. Variations of wood properties between plus-tree clones in Larix kaempferi(Lamb.)Carrière [J]. Bulletin of the Forest Tree Breeding Center, 2005, 21: 85-105
[9] WATANABE M, WATANABE Y, KITAOKA S, et al. Growth and photosynthetic traits of hybrid larch F1(Larix gmelinii var. japonica × L. kaempferi)under elevated CO2 concentration with low nutrient availability[J]. Tree Physiology, 2011, 31(9): 965-975. DOI:10.1093/treephys/tpr059.
[10] HAN H, SUN X M, XIE Y H, et al. Transcriptome and proteome profiling of adventitious root development in hybrid larch(Larix kaempferi &#215; Larix olgensis)[J]. BMC Plant Biology, 2014, 14: 305. DOI:10.1186/s12870-014-0305-4.
[11] MORIGUCHI Y, KITA K, UCHIYAMA K, et al. Enhanced hybridization rates in a Larix gmelinii var. japonica &#215; L. kaempferi interspecific seed orchard with a single maternal clone revealed by cytoplasmic DNA markers[J]. Tree Genetics & Genomes, 2008, 4(4): 637-645. DOI:10.1007/s11295-008-0139-z.
[12] 赖猛. 落叶松无性系遗传评价与早期选择研究[D]. 北京: 中国林业科学研究院, 2014.
LAI M. Genotypic evaluation and early selection of Larix clones[D]. Beijing: Chinese Academy of Forestry, 2014.
[13] 武朋辉, 刘佳, 陈小超, 等. 油松全同胞家系苗期生长性状遗传变异研究[J]. 陕西林业科技, 2012(4): 23-26,32. DOI:10.3969/j.issn.1001-2117.2012.04.007.
WU P H, LIU J, CHEN X C, et al. Genetic variations of seedling growth traits among full-sib families of Pinus tabuleaformis[J]. Shaanxi Forest Science and Technology, 2012(4): 23-26,32.
[14] 黄云鹏. 樟树家系子代测定林试验的初步研究[J]. 三明学院学报, 2008, 25(4): 426-431. DOI:10.3969/j.issn.1673-4343.2008.04.017.
HUANG Y P. Studies on Cinnamomum camphora(L.)Presl pedigree in test forest[J]. Journal of Sanming University, 2008, 25(4): 426-431.
[15] ZHAO X Y, XIA H, WANG X W, et al. Variance and stability analyses of growth characters in half-sib Betula platyphylla families at three different sites in China[J]. Euphytica, 2016, 208(1): 173-186. DOI:10.1007/s10681-015-1617-7.
[16] MARRON N, CEULEMANS R. Genetic variation of leaf traits related to productivity in a Populus deltoides &#215; Populus nigra family[J]. Canadian Journal of Forest Research, 2006, 36(2): 390-400. DOI:10.1139/x05-245.
[17] HANSEN J, ROULUND H. Genetic parameters for spiral grain, stem form, pilodyn and growth in 13 years old clones of Sitka Spruce(Picea sitchensis(Bong.)Carr.)[J]. Silvae Genet, 1997, 46: 107-113.
[18] 陈晓阳,沈熙环. 林木育种学[M]. 北京:高等教育出版社,2005.
CHEN X Y, SHEN X H. Forest tree breeding [M]. Beijing: Higher Education Press,2005.
[19] 续九如. 林木数量遗传学[M]. 北京: 中国林业出版社,2006: 31,55.
XU J R.Tree population genetics [M]. Beijing: China Forestry Publishing House, 2006: 31, 55.
[20] METOUGUI M L, MOKHTATI M, MAUGHAN P, et al. Morphological variability, heritability and correlation studies within an argan tree population(Argania spinosa(L.)Skeels)preserved in situ[J]. International Journal of Agriculture and Forestry, 2017, 7(2): 42-51.DOI: 10.5923/j.ijaf.20170702.02.
[21] PAN Y Y, LI S C, WANG C L, et al. Early evaluation of growth traits of Larix kaempferi clones[J]. Journal of Forestry Research, 2018, 29(4): 1031-1039. DOI:10.1007/s11676-017-0492-6.
[22] 朱之悌. 林木遗传学基础[M]. 北京: 中国林业出版社, 1990.
ZHU Z D. The basic of tree genetics[M]. Beijing: China Forestry Publishing House, 1990.
[23] LIANG D Y, DING C J, ZHAO G H, et al. Variation and selection analysis of Pinus koraiensis clones in northeast China [J]. Journal of Forestry Research, 2018(3): 1-12.DOI:10.1007/s11676-017-0471-y.
[24] 贺义才. 落叶松种和种源选择研究[J]. 山西林业科技, 2015, 44(4): 23-25. DOI:10.3969/j.issn.1007-726X.2015.04.007.
HE Y C. Selection study on species and provenance of Larix gmelinii[J]. Shanxi Forestry Science and Technology, 2015, 44(4): 23-25.
[25] MWASE W F, SAVILL P S, HEMERY G. Genetic parameter estimates for growth and form traits in common ash(Fraxinus excelsior L.)in a breeding seedling orchard at Little Wittenham in England[J]. New Forests, 2008, 36(3): 225-238. DOI:10.1007/s11056-008-9095-6.
[26] 高玉池. 白桦种源遗传多样性研究[D]. 哈尔滨: 东北林业大学, 2010.
GAO Y C. Genetic diversity of provenances of Betula platyphylla [D]. Harbin: Northeast Forestry University, 2010.
[27] 贾庆彬, 张含国, 张磊, 等. 杂种落叶松优良家系选择与生长节律分析[J]. 北京林业大学学报, 2016, 38(2): 52-60. DOI:10.13332/j.1000-1522.20150343.
JIA Q B, ZHANG H G, ZHANG L, et al. Selection of superior hybrid larch families and growth rhythm analysis[J]. Journal of Beijing Forestry University, 2016, 38(2): 52-60.
[28] 王志波, 季蒙, 张银昌, 等. 华北落叶松1年生播种苗生长规律研究[J]. 林业科技, 2016, 41(3): 4-6. DOI:10.3969/j.issn.1001-9499.2016.03.002.
WANG Z B, JI M, ZHANG Y C, et al. Research on yearly growth regularity of one-year-old seedlings of Larix principis-rupprechtii[J]. Forestry Science & Technology, 2016, 41(3): 4-6.
[29] 黄以法. 马尾松全同胞子代遗传变异与优良单株评选[J]. 三明学院学报, 2017, 34(4): 15-19. DOI:10.14098/j.cn35-1288/z.2017.04.003.
HUANG Y F. Genetic variation and selection of plus trees in Pinus massoniana full-sib progenies[J]. Journal of Sanming University, 2017, 34(4): 15-19.
[30] 梁德洋, 金允哲, 赵光浩, 等. 50个红松无性系生长与木材性状变异研究[J]. 北京林业大学学报, 2016, 38(6): 51-59. DOI:10.13332/j.1000-1522.20150465.
LIANG D Y, JIN Y Z, ZHAO G H, et al. Variance analyses of growth and wood characteristics of 50 Pinus koraiensis clones[J]. Journal of Beijing Forestry University, 2016, 38(6): 51-59.
[31] 秦光华, 姜岳忠, 乔玉玲, 等. 黑杨派杨树杂交F1子代苗期遗传测定[J]. 东北林业大学学报, 2011, 39(4): 29-32. DOI:10.3969/j.issn.1000-5382.2011.04.010.
QIN G H, JIANG Y Z, QIAO Y L, et al. Genetic testing of F1 hybrid progeny of aigeiros section at seedling stage [J]. Journal of Northeast Forestry University, 2011, 39(4): 29-32.
[32] 方海峰, 丁振芳, 丁琳琳, 等. 日本落叶松全同胞子代生长性状的分析[J]. 辽宁林业科技, 2010(3): 17-20. DOI:10.3969/j.issn.1001-1714.2010.03.005.
FANG H F, DING Z F, DING L L, et al. Analysis of growth characters of full-sib progeny of Larix kaempferi[J]. Liaoning Forestry Science and Technology, 2010(3): 17-20.
[33] 陈代喜, 陈晓明, 蓝肖, 等. 杉木全同胞子代遗传测定与优良种质选择[J]. 广西林业科学, 2016, 45(4): 347-351. DOI:10.3969/j.issn.1006-1126.2016.04.001.
CHEN D X, CHEN X M, LAN X, et al. Full-sib progeny genetic test and selection of superior germ plasm of Cunninghamia lanceolata [J]. Guangxi Forestry Science, 2016, 45(4): 347-351.
[34] 谭健晖, 冯源恒, 黄永利, 等. 26年生马尾松初级种子园半同胞子代变异及家系选择[J]. 南京林业大学学报(自然科学版), 2017, 41(3): 189-192. DOI:10.3969/j.issn.1000-2006.201507061.
TAN J H, FENG Y H, HUANG Y L, et al. Open pollination progeny test and family selection of 26-year-old Pinus massoniana Lamb. seed orchard[J]. Journal of Nanjing Forestry University(Natural Sciences Edition), 2017, 41(3): 189-192.
[35] 徐焕文, 刘宇, 李志新, 等. 5年生白桦杂种子代多点稳定性分析及优良家系选择[J]. 北京林业大学学报, 2015, 37(12): 24-31. DOI:10.13332/j.1000-1522.20140466.
XU H W, LIU Y, LI Z X, et al. Analysis of the stability and superiority of five-year-old birch crossbreed families based on a multi-site test[J]. Journal of Beijing Forestry University, 2015, 37(12): 24-31.
[36] 刘宇, 徐焕文, 滕文华, 等. 白桦全同胞子代测定及优良家系早期选择[J]. 北京林业大学学报, 2017, 39(2): 1-8. DOI:10.13332/j.1000-1522.20160138.
LIU Y, XU H W, TENG W H, et al. Full-sib progeny test and early selection in superior families of Betula platyphylla[J]. Journal of Beijing Forestry University, 2017, 39(2): 1-8.
[37] 杨秀艳, 张守攻, 孙晓梅, 等. 北亚热带高山区日本落叶松自由授粉家系遗传测定与二代优树选择[J]. 林业科学, 2010, 46(8): 45-50. DOI:10.11707/j.1001-7488.20100807.
YANG X Y, ZHANG S G, SUN X M, et al. Genetic test of open-pollinated Larix kaempferi families and selection for the second generation elite trees in northern sub-tropical alpine area[J]. Scientia Silvae Sinicae, 2010, 46(8): 45-50.


 WANG Jun-hui,ZHANG Shou-gong,SHI Shu-lan,et al.The Physical Properties of Larix kaempferi Pulp[J].Journal of Nanjing Forestry University(Natural Science Edition),2005,29(02):041.[doi:10.3969/j.jssn.1000-2006.2005.03.010]
 MA Shun-xing,WANG Jun-hui*,ZHANG Shou-gong,et al.A Study on Genetic Variation of Wood Tissue Proportion of Japanese Larch Clones[J].Journal of Nanjing Forestry University(Natural Science Edition),2006,30(02):055.[doi:10.3969/j.jssn.1000-2006.2006.05.013]
 ZHAO Fencheng,GUO Wenbing,LIN Changming,et al.Effects of different inbreeding levels on seed characteristics and growth of slash pine[J].Journal of Nanjing Forestry University(Natural Science Edition),2019,43(02):009.[doi:10.3969/j.issn.1000-2006.201712043]


收稿日期:2018-03-27 修回日期:2018-07-06
更新日期/Last Update: 2019-03-30