[1]郑孙元,朱 弘,金思雨,等.桂花表型变化的环境依赖特征[J].南京林业大学学报(自然科学版),2019,43(02):038-46.[doi:10.3969/j.issn.1000-2006.201808039]
 ZHENG Sunyuan,ZHU Hong,JIN Siyu,et al.Environment-dependent phenotypic variation of Osmanthus fragrans [J].Journal of Nanjing Forestry University(Natural Science Edition),2019,43(02):038-46.[doi:10.3969/j.issn.1000-2006.201808039]
点击复制

桂花表型变化的环境依赖特征
分享到:

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

卷:
43
期数:
2019年02期
页码:
038-46
栏目:
研究论文
出版日期:
2019-03-30

文章信息/Info

Title:
Environment-dependent phenotypic variation of Osmanthus fragrans
文章编号:
1000-2006(2019)02-0038-09
作者:
郑孙元1朱 弘1金思雨2王梦娟2孙 杰2谷诚诚2 裴引祎2王贤荣1*段一凡1*
(1.南京林业大学生物与环境学院,江苏 南京 210037; 2.南京林业大学林学院,江苏 南京 210037)
Author(s):
ZHENG Sunyuan1 ZHU Hong1 JIN Siyu2 WANG Mengjuan2 SUN Jie2 GU Chengcheng2 PEI Yinwei2 WANG Xianrong1* DUAN Yifan1*
(1. College of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, China; 2. College of Forestry, Nanjing Forestry University, Nanjing 210037, China)
关键词:
桂花 表型变异 表型多样性 地理-气候环境因子相关性
Keywords:
Osmanthus fragrans phenotypic variation phenotypic diversity geography-climate environmental factor correlation
分类号:
S685.13
DOI:
10.3969/j.issn.1000-2006.201808039
文献标志码:
A
摘要:
【目的】揭示桂花(Osmanthus fragrans Lour.)的表型分化程度和地理变异规律。【方法】通过聚类分析、方差分析、相关性分析等方法研究26个桂花居群叶表型及9个桂花居群花表型变异情况及与地理-气候环境因子的相关性。【结果】桂花叶表型性状存在相当程度的变异,叶片8个性状变异程度各有差异; 根据方差分析结果,居群间变异占75.04%, 居群内占13.28%,居群间的变异是主要的变异来源。以地区而言,平均变异系数为19.08%,变异系数最大的是湖北桂花镇居群(29.73%),而贵州夫子坝居群最小(13.68%)。就花表型而言,花序数目及花梗长变异离散程度最大。桂花与经纬度、温度季节性变化方差、最热月最高温度等7个环境影响因子存在显著或极显著相关性。叶先端角度呈现由西向东减小的趋势,花瓣性状呈现由南到北逐渐变大的趋势。聚类分析结果可以明显将26个群体分成3组。【结论】桂花表型数量性状的变异相当丰富,且与地理-气候环境因子的相关性较密切,研究结果可以为桂花种质资源的保护和利用奠定基础。
Abstract:
【Objective】Reveal the phenotypic diversity and geographical variation patterns of Osmanthus fragrans.【Method】Cluster analysis variance analysis and correlation analysis were used to study the leaf phenotypic traits of 26 O. fragrans populations, the flower phenotypic traits of 9 O. fragrans populations, and their variability and correlation with geographical-climatic factors. 【Result】The phenotypic traits of O. fragrans were variable and there were differences in the degree of variation of the eight leaf characteristics. According to the results of the analysis of variance, the phenotypic variation was 75.04% among populations and 13.28% within populations. The main source of variation was from among the populations. In terms of the regions, the average coefficient of variation was 19.08%, and the largest coefficient of variation occurred in the Guihua Town population(29.73%)from the Hubei Province, while the Fuziba population from the Guizhou Province was the smallest(13.68%). In terms of floral phenotypes, the number of inflorescences and pedicels showed the greatest degree of discrepancies. O. fragrans has significant or extremely significant correlations with seven environmental impact factors, such as the latitude, longitude, temperature seasonality, and max temperature of the warmest month. There was a significant or extremely significant correlation between all types of traits. The apex angle of the leaves showed a trend of decreasing from west to east, while the petal traits showed an increasing trend from south to north. The cluster analysis results showed that 26 populations could be clearly divided into 3 groups.【Conclusion】There is a high degree of variation and a rich diversity in the phenotypic traits of O. fragrans. Additionally, O. fragrans are closely related to geo-climatic factors. These results can lay the foundation for the protection and innovation of O. fragrans germplasm resources.

参考文献/References:


[1] PIGLIUCCI M. Phenotypic plasticity and evolution by genetic assimilation[J]. Journal of Experimental Biology, 2006, 209(12): 2362-2367. DOI:10.1242/jeb.02070.
[2] LI Y G, LIU X H, MA J W, et al. Phenotypic variation in Phoebe bournei populations preserved in the primary distribution area[J]. Journal of Forestry Research, 2018, 29(1): 35-44. DOI:10.1007/s11676-017-0409-4.
[3] 王姝, 周道玮. 植物表型可塑性研究进展[J]. 生态学报, 2017, 37(24): 8161-8169. DOI:10.5846/stxb201611242412.
WANG S, ZHOU D W. Research on phenotypic plasticity in plants: an overview of history, current status, and development trends[J]. Acta Ecologica Sinica, 2017, 37(24): 8161-8169.
[4] 董昕, 王磊, 鲁仪增, 等. 山东稀有植物小果白刺天然群体表型变异研究[J]. 林业科学研究, 2017, 30(2): 293-299. DOI:10.13275/j.cnki.lykxyj.2017.02.015.
DONG X, WANG L, LU Y Z, et al. Phenotypic variation of nitraria sibirica natural populations in Shandong[J]. Forest Research, 2017, 30(2): 293-299.
[5] 彭丽平, 成仿云, 钟原, 等. 凤丹栽培群体的表型变异研究[J]. 植物科学学报, 2018, 36(2): 170-180. DOI:10.11913/PSJ.2095-0837.2018.20170.
PENG L P, CHENG F Y, ZHONG Y, et al. Phenotypic variation in cultivar populations of Paeonia ostii T. Hong et J. X. Zhang[J]. Plant Science Journal, 2018, 36(2): 170-180.
[6] 柳江群, 尹明宇, 左丝雨, 等. 长柄扁桃天然种群表型变异[J]. 植物生态学报, 2017, 41(10): 1091-1102. DOI:10.17521/cjpe.2017.0104.
LIU J Q, YIN M Y, ZUO S Y, et al. Phenotypic variations in natural populations of Amygdalus pedunculata[J]. Acta Phytoecologica Sinica, 2017, 41(10): 1091-1102.
[7] 尹明宇, 朱绪春, 刘慧敏, 等. 西伯利亚杏种质资源花表型变异[J]. 西北农林科技大学学报(自然科学版), 2018, 46(2): 92-103. DOI:10.13207/j.cnki.jnwafu.2018.02.012.
YIN M Y, ZHU X C, LIU H M, et al. Flower phenotypic variations of germplasm resources of Siberian apricot(Armeniaca sibirica)[J]. Journal of Northwest A & F University(Natural Science Edition), 2018, 46(2): 92-103.
[8] MOZDZER T J, ZIEMAN J C. Ecophysiological differences between genetic lineages facilitate the invasion of non-native Phragmites australis in North American Atlantic coast wetlands[J]. Journal of Ecology, 2010, 98(2): 451-458. DOI:10.1111/j.1365-2745.2009.01625.x.
[9] 徐沂春,赵宏波,胡绍庆. 分布南界和北界的桂花野生种群遗传多样性比较[C]//张启翔. 2014 中国观赏园艺研究进展.北京:中国林业出版社,2014:29-35.
XU Y C, ZHAO H B, HU S Q. Comparison of genetic diversity of two populations from the northern and southern boundary of natural distribution in Osmanthus fragrans[C]//ZHANG Q X. Progress of Ornamental Horticulture in China(2014). Beijing:China Forestry Publishing House, 2014:29-35.
[10] WANG J Y, YUAN J H, MEI F D, et al. Assessment of genetic diversity and relationships among Osmanthus fragrans cultivars using AFLP markers[J]. Electronic Journal of Biotechnology, 2011, 14(1): 2-3. DOI:10.2225/vol14-issue1-fulltexT-9.
[11] 韩远记, 董美芳, 袁王俊, 等. 部分桂花栽培品种的AFLP分析[J]. 园艺学报, 2008, 35(1): 137-142. DOI:10.3321/j.issn:0513-353X.2008.01.024.
HAN Y J, DONG M F, YUAN W J, et al. Studies on the genetic diversity of some Osmanthus fragrans cultivars based on AFLP markers[J]. Acta Horticulturae Sinica, 2008, 35(1): 137-142.
[12] 韩远记, 董美芳, 袁王俊, 等. 桂花品种资源的遗传多样性分析[J]. 植物学通报, 2008, 25(5): 559-564. DOI:10.3969/j.issn.1674-3466.2008.05.007.
HAN Y J, DONG M F, YUAN W J, et al. Study on the genetic diversity of Osmanthus fragrans cultivars[J]. Chinese Bulletin of Botany, 2008, 25(5): 559-564.
[13] DUAN Y F, LI M, YI X G, et al. Assessment of genetic diversity among androdioecious ancient Osmanthus fragrans trees by SSR markers[J]. Biochemical Systematics and Ecology, 2015, 61: 179-185. DOI:10.1016/j.bse.2015.06.009.
[14] 赵宏波, 郝日明, 胡绍庆. 中国野生桂花的地理分布和种群特征[J]. 园艺学报, 2015, 42(9): 1760-1770. DOI:10.16420/j.issn.0513-353x.2014-0939.
ZHAO H B, HAO R M, HU S Q. Geographic distribution and population characteristics of Osmanthus fragrans[J]. Acta Horticulturae Sinica, 2015, 42(9): 1760-1770.
[15] 胡坚强, 夏有根, 梅艳, 等. 古树名木研究概述[J]. 福建林业科技, 2004, 31(3): 151-154. DOI:10.3969/j.issn.1002-7351.2004.03.042.
HU J Q, XIA Y G, MEI Y, et al. Research of the acient and famouse trees in China[J]. Journal of Fujian Forestry Science and Technology, 2004, 31(3): 151-154.
[16] 葛颂, 王明庥, 陈岳武. 用同工酶研究马尾松群体的遗传结构[J]. 林业科学,1988, 24(4): 399-409.
GE S, WANG M X, CHEN Y W. Study on the genetic structure of masson pine population by isoenzyme[J]. Scientia Silvae Sinicae,1988,24(4): 399-409.
[17] JOSHI J, SCHMID B, CALDEIRA M C, et al. Local adaptation enhances performance of common plant species[J]. Ecology Letters, 2001, 4(6): 536-544. DOI:10.1046/j.1461-0248.2001.00262.x.
[18] BANTA J A, DOLE J, CRUZAN M B, et al. Evidence of local adaptation to coarse-grained environmental variation in Arabidopsis thaliana[J]. Evolution, 2007, 61(10): 2419-2432. DOI:10.1111/j.1558-5646.2007.00189.x.
[19] TSOBENG A, TCHOUNDJEU Z, DEGRANDE A, et al. Phenotypic variation in Pentaclethra macrophylla Benth(Fabaceae)from the humid lowlands of Cameroon[J]. Afrika Focus, 2015, 28(2): 47-61. DOI:10.21825/af.v28i2.4864.
[20] FLÜCKIGER W, OERTLI J J, FLÜCKIGER-KELLER H. The effect of wind gusts on leaf growth and foliar water relations of aspen[J]. Oecologia, 1978, 34(1): 101-106. DOI:10.1007/bf00346244.
[21] 彭珠清. 闽台杉木种质资源亲缘关系的比较研究[D]. 福州: 福建农林大学, 2013.
PENG Z Q. Comparative study of Chinese fir germplasm resources genetic relationship between Fujian and Taiwan[D]. Fuzhou: Fujian Agriculture and Forestry University, 2013.
[22] 张凯, 侯继华, 何念鹏. 油松叶功能性状分布特征及其控制因素[J]. 生态学报, 2017, 37(3): 736-749.DOI:10.5846/stxb201508291793.
ZHANG K, HOU J H, HE N P. Leaf functional trait distribution and controlling factors of Pinus tabuliformis[J]. Acta Ecologica Sinica, 2017, 37(3): 736-749.
[23] 尹明宇. 内蒙古西伯利亚杏遗传变异及优良种源、家系、单株选择[D]. 北京: 中国林业科学研究院, 2017.
YI M Y. High-level genetic variation and superior provenances, families, and individuals selection of Armeniaca sibirica in Inner Mongolia[D]. Beijing: Chinese Academy of Forestry, 2017.
[24] 文珊娜. 灰木莲种质资源遗传多样性研究[D]. 北京: 中国林业科学研究院, 2017.
WEN S N. Genetic diversity of germplasm resourse of Manglietia conifera Dandy[D]. Beijing: Chinese Academy of Forestry, 2017.
[25] 惠利省. 马褂木遗传多样性及系统地理学研究[D]. 南京: 南京林业大学, 2010.
HUI L S. Genetic diversity and phylogeography of Liriodendron chinense[D]. Nanjing: Nanjing Forestry University, 2010.
[26] 姚淑均. 滇楸优树及其子代苗期性状遗传变异研究[D]. 北京: 中国林业科学研究院, 2013.
YAO S J. Study of seedling traits varying genetically of excellent tree and its progenies in Catalpa fgargesii bur. f. duclouxii(Dode)Gilmour[D]. Beijing: Chinese Academy of Forestry, 2013.
[27] 邵文豪, 汤庚国, 姜景民. 基于蜡叶标本分析的湖北海棠种内变异与地理分化研究[J]. 植物研究, 2009, 29(3): 270-275.
SHAO W H, TANG G G, JIANG J M. Intraspecific variation and geographical differentiation of Malus hupehensis based on exsiccate-specimen analysis[J]. Bulletin of Botanical Research, 2009, 29(3): 270-275.

相似文献/References:

[1]王良桂,李霞,杨秀莲.控根栽培下桂花根系的动态生长与垂直分布特征[J].南京林业大学学报(自然科学版),2011,35(04):043.[doi:10.3969/j.jssn.1000-2006.2011.04.009]
 WANG Lianggui,LI Xia,YANG Xiulian.The growing dynamics and vertical distribution feature of Osmanthus fragrans seedling root system under the rootcontrolling cultivation[J].Journal of Nanjing Forestry University(Natural Science Edition),2011,35(02):043.[doi:10.3969/j.jssn.1000-2006.2011.04.009]
[2]刘玉莲.南京地区桂花栽培品种调查研究[J].南京林业大学学报(自然科学版),1985,9(01):030.[doi:10.3969/j.jssn.1000-2006.1985.01.004]
 Liu Yulian.PRELIMINARY STUDY OF SWEET OSMANTHUS IN NANJING AREA[J].Journal of Nanjing Forestry University(Natural Science Edition),1985,9(02):030.[doi:10.3969/j.jssn.1000-2006.1985.01.004]
[3]杨春材,赵莉,刘兰英.马氏粉虱AIeuroclobusmarlattif(Ouaintance)的初步研究[J].南京林业大学学报(自然科学版),1996,20(02):092.[doi:10.3969/j.jssn.1000-2006.1996.02.019]
[4]刘龙昌,向其柏.木犀属植物的研究进展[J].南京林业大学学报(自然科学版),2003,27(02):084.[doi:10.3969/j.jssn.1000-2006.2003.02.020]
 LIU Long chang,XIANG Qi baiCollege of Forest Resources and Environment Nanjing Forestry University,Nanjing 007,et al.Research Progress on Osmanthus Genus[J].Journal of Nanjing Forestry University(Natural Science Edition),2003,27(02):084.[doi:10.3969/j.jssn.1000-2006.2003.02.020]
[5]向其柏,臧德奎,刘玉莲,等.桂花新品种[J].南京林业大学学报(自然科学版),2004,28(07):014.[doi:10.3969/j.jssn.1000-2006.2004.07.003]
 XIANG Qi-bai,ZANG De-kui,LIU Yu-lian,et al.New Cultivars of Sweet Osmanthus[J].Journal of Nanjing Forestry University(Natural Science Edition),2004,28(02):014.[doi:10.3969/j.jssn.1000-2006.2004.07.003]
[6]汪小飞,史佑海,王芳.四川桂花品种资源调查及开发利用[J].南京林业大学学报(自然科学版),2004,28(07):026.[doi:10.3969/j.jssn.1000-2006.2004.07.004]
 WANG Xiao-fei,SHI You-hai,WANG Fang.The Investigation on Cultivars Resources and Application of Sweet Osmanthus in Sichuan Province[J].Journal of Nanjing Forestry University(Natural Science Edition),2004,28(02):026.[doi:10.3969/j.jssn.1000-2006.2004.07.004]
[7]王贤荣,袁发银,陈昕,等.湖南浏阳桂花峡桂花次生林的物种数量特征[J].南京林业大学学报(自然科学版),2004,28(07):041.[doi:10.3969/j.jssn.1000-2006.2004.07.007]
 WANG Xian-rong,YUAN Fa-yin,CHEN Xin,et al.Quantitative Characteristics of Species in Wild Community of Osmanthus fragrans in Guihuaxia,Liuyang,Hunan[J].Journal of Nanjing Forestry University(Natural Science Edition),2004,28(02):041.[doi:10.3969/j.jssn.1000-2006.2004.07.007]
[8]伊艳杰,黄莹,尚富德.利用RAPD标记研究桂林桂花品种间的亲缘关系(英文)[J].南京林业大学学报(自然科学版),2004,28(07):065.[doi:10.3969/j.jssn.1000-2006.2004.07.010]
 YI Yan-jie,HUANG Ying,SHANG Fu-de.Identifying Osmanthus fragrans Cultivars in Guilin City and Evaluating Their Genetic Relationships by RAPD Markers[J].Journal of Nanjing Forestry University(Natural Science Edition),2004,28(02):065.[doi:10.3969/j.jssn.1000-2006.2004.07.010]
[9]胡绍庆,邱英雄,吴光洪,等.桂花品种的ISSR-PCR分析[J].南京林业大学学报(自然科学版),2004,28(07):071.[doi:10.3969/j.jssn.1000-2006.2004.07.011]
 Hu Shao-qing,Qiu Ying-xiong,Wu Guang-hong,et al.A ISSR-PCR Analyze of the Osmanthus fragrans Cultivars[J].Journal of Nanjing Forestry University(Natural Science Edition),2004,28(02):071.[doi:10.3969/j.jssn.1000-2006.2004.07.011]
[10]刘龙昌,向其柏,刘玉莲.RAPD标记在桂花遗传多样性检测和品种鉴定中的应用(英文)[J].南京林业大学学报(自然科学版),2004,28(07):076.[doi:10.3969/j.jssn.1000-2006.2004.07.012]
 LIU Long-chang,XIANG Qi-bai,LIU Yu-lian.The Application of RAPD Markers in Diversity Detection and Cultivars Identification of Osmanthus fragrans[J].Journal of Nanjing Forestry University(Natural Science Edition),2004,28(02):076.[doi:10.3969/j.jssn.1000-2006.2004.07.012]

备注/Memo

备注/Memo:
收稿日期:2018-08-17 修回日期:2019-01-02
基金项目:中国博士后科学基金项目(2016M590462); 江苏省自然科学基金青年基金项目(BK20160932); 江苏省高校自然科学研究面上项目(15KJB180007)。
第一作者:郑孙元(1622262996@qq.com),ORCID(0000-0002-1541-1541)。
*通信作者:王贤荣,教授,博士,负责论文框架设定与修改,ORCID(0000-0002-7940-909X)。段一凡(yifan419@hotmail.com),副教授,博士,负责指导项目的具体实施,ORCID(0000-0002-1834-9337)。
更新日期/Last Update: 2019-03-30