[1]黄东静,曹福亮,汪贵斌*.无机氮素形态对银杏苗期碳氮代谢的影响[J].南京林业大学学报(自然科学版),2019,43(02):197-202.[doi:10.3969/j.issn.1000-2006.201807043]
 HUANG Dongjing,CAO Fuliang,WANG Guibin*.Nitrogen form and ratio affect the carbon-nitrogen metabolism of Ginkgo biloba[J].Journal of Nanjing Forestry University(Natural Science Edition),2019,43(02):197-202.[doi:10.3969/j.issn.1000-2006.201807043]
点击复制

无机氮素形态对银杏苗期碳氮代谢的影响
分享到:

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

卷:
43
期数:
2019年02期
页码:
197-202
栏目:
研究简报
出版日期:
2019-03-30

文章信息/Info

Title:
Nitrogen form and ratio affect the carbon-nitrogen metabolism of Ginkgo biloba
文章编号:
1000-2006(2019)02-0197-06
作者:
黄东静曹福亮汪贵斌*
(南京林业大学南方现代林业协同创新中心,南京林业大学林学院,江苏 南京 210037)
Author(s):
HUANG Dongjing CAO Fuliang WANG Guibin*
(Co-Innovation Center for the Sustainable Forestry in Southern China, College of Forestry,Nanjing Forestry University, Nanjing 210037, China)
关键词:
银杏 硝态氮 铵态氮 碳氮代谢
Keywords:
Ginkgo biloba nitrate nitrogen ammonium nitrogen carbon-nitrogen metabolite
分类号:
S792.95
DOI:
10.3969/j.issn.1000-2006.201807043
文献标志码:
A
摘要:
【目的】研究不同氮素形态配比施肥对银杏碳氮代谢的影响,为银杏合理施肥提供理论依据。【方法】以1年生银杏实生苗为材料,采用温室盆栽试验,设置5种不同铵硝比[m(NH+4-N):m(NO-3-N)]处理,分别为T1(100:0)、T2(75:25)、T3(50:50)、T4(25:75)和T5(0:100),分析了不同处理下银杏叶片全氮、可溶性蛋白、游离氨基酸、全碳、可溶性糖、淀粉和蔗糖含量以及淀粉酶(AMS)、蔗糖合成酶(SS)、蔗糖磷酸合成酶(SPS)、硝酸还原酶(NR)、谷氨酰胺合成酶(GS)和谷氨酸合成酶(GOGAT)活性的变化规律。【结果】随着NO-3-N比例的增加,银杏幼苗叶片碳氮代谢物含量和相关酶活性总体上(除可溶性蛋白含量)呈先增后减的变化趋势。碳氮代谢物方面,T3处理的蔗糖和游离氨基酸含量最高,T4处理的全氮、全碳、可溶性糖、可溶性蛋白、淀粉和蔗糖含量均最高,而T1处理除可溶性蛋白含量外,剩余碳氮代谢物的含量均最低。酶活性方面,T3处理的SS和SPS活性最高,T4处理的AMS、NR、GS和GOGAT活性均最高,而T1处理的AMS、SS和NR活性均最低,T5处理的SPS、GS和GOGAT活性均最低。总体上,T3和T4处理碳氮代谢物含量和酶活性均显著高于其他处理,但T3与T4处理间无显著差异。【结论】NH+4-N和NO-3-N混合施用增强了银杏的碳氮代谢能力,在当前土壤条件下,m(NH+4-N):m(NO-3-N)为25:75时效果最佳,在生产中应根据实际情况进行适当的调整。
Abstract:
【Objective】The effects of nitrogen form and ratio on carbon-nitrogen metabolism and related enzyme activities in the leaves of Ginkgo biloba seedlings were investigated to provide a theoretical basis for N fertilizer management of Ginkgo biloba. 【Method】Using 1-year-old seedlings of Ginkgo biloba as test materials, a greenhouse pot experiment was performed with 5 ratios of m(NH4+-N):m(NO3-N), namely T1(100:0), T2(75:25), T3(50:50), T4(25:75), and T5(0:100). We analyzed the content of total nitrogen, soluble protein, free amino acid, total carbon, soluble sugar, starch and sucrose, and the activity of amylase(AMS), sucrose synthetase(SS), sucrose phosphate synthase(SPS), nitrate reductase(NR), glutamine synthetase(GS), and glutamine synthetase(GOGAT)in the Ginkgo biloba seedlings under different treatments. 【Result】The carbon-nitrogen metabolites(except soluble protein content)and related enzyme activities in the leaves of Ginkgo biloba increased initially and then decreased, with an increase in the NO3--N ratio. In terms of carbon-nitrogen metabolism, the content of sucrose and free amino acids were the highest in the T3 group, and the content of total nitrogen, total carbon, soluble sugar, soluble protein, starch, and sucrose were the highest in the T4 group. The content of carbon-nitrogen metabolism indicators, except for soluble protein content, were the lowest in the T1 group. In terms of enzyme activity, SS and SPS activities were the highest in the T3 group, and AMS, NR, GS and GOGAT activities were the highest in the T4 group. AMS, SS, and NR activities were the lowest in the T1 group, and SPS, GS and GOGAT activities were the lowest in the T5 group. Overall, the contents of carbon-nitrogen metabolites and related enzyme activities in T3 and T4 groups were significantly higher than that in other groups, but no significant differences were observed between the T3 and T4 groups. 【Conclusion】The effects of NH4+-N and NO3--N mixed supply on the carbon-nitrogen metabolism of Ginkgo biloba were significantly higher than those of the single supply. The effect of 25:75 m(NH4+-N):m(NO3--N)was the best, and appropriate adjustments should be performed on the basis of the conditions.

参考文献/References:


[1] 赵平, 彭少麟. 植物氮素营养的生理生态学研究[J]. 生态科学, 1998, 17(2):37-42.
ZHAO P, PENG S L. Ecophysiological research on nitrogen nutrition of plant[J]. Ecologic Science, 1998, 17(2):37-42.
[2] 叶莉莎, 陈双林. 氮素形态及配比对雷竹光合特性和氮代谢酶活性的影响[J]. 生态学杂志, 2016, 35(9):2355-2360. DOI: 10.13292/j.1000-4890.201609.025.
YE L S, CHEN S L.Effects of nitrogen forms and ratios on photosynthetic characters and enzyme activities in nitrogen metabolism of Phyllostachys violascens[J]. Chinese Journal of Ecology, 2016, 35(9):2355-2360.
[3] 李安亮. 不同油茶品种对氮素形态的响应研究[D]. 长沙:中南林业科技大学, 2016.
LI A L. Research on the response of different Camellia oleifera varieties to nitrogen forms[D]. Changsha: Central South University of Forestry and Technology, 2016.
[4] 孙世芹, 阎秀峰. 氮素形态对喜树幼苗喜树碱含量及喜树碱代谢相关酶类的影响[J]. 中国中药杂志, 2008, 33(13):1519-1523.
SUN S Q, YAN X F. Effects of nitrogen forms on camptothecin content and camptothecin metabolism related enzymes in Camptotheca acuminata seedlings[J]. China Journal of Chinese Materia Medica, 2008, 33(13):1519-1523.
[5] 和阳, 王兴东. 不同氮素形态对蓝莓叶片矿质元素含量的影响[J]. 北方园艺, 2009(9):58-59.
HE Y, WANG X D. Effect of nitrogen form on the leaf element content of blueberry[J]. Northern Horticulture, 2009(9):58-59.
[6] 李亚东, 赵爽, 张志东,等. 不同氮素形态配比对越橘生长、产量及叶片元素含量的影响[J]. 吉林农业大学学报, 2008, 30(4):477-480.
LI Y D, ZHAO S, ZHANG Z D, et al. Effect of nitrogen form ratios on the growth, yield and leaf element content of blueberry[J]. Journal of Jilin Agricultural University, 2008, 30(4):477-480.
[7] 李霞, 阎秀峰, 刘剑锋,等. 氮素形态对黄檗幼苗生长及氮代谢相关酶类的影响[J]. 植物学报, 2006, 23(3):255-261. DOI: 10.3969/j.issn.1674-3466.2006.03.004.
LI X, YAN X F, LIU J F,et al. Effects of nitrogen forms on nitrogen metabolism-related enzymes and growth of Phellodendron amurense seedlings[J]. Chinese Bulletin of Botany, 2006, 23(3):255-261.
[8] 周鹏, 彭福田, 魏绍冲,等. 氮素形态对平邑甜茶细胞分裂素水平和叶片生长的影响[J]. 园艺学报, 2007, 34(2):269-274. DOI: 10.3321/j.issn:0513-353X.2007.02.001.
ZHOU P, PENG F T, WEI S C, et al. Effects of rhizosphere nitrate and ammonium on the level of cytokinins and leaf growth of Malus hupenensis R[J]. Acta Horticulturae Sinica, 2007, 34(2):269-274.
[9] 唐辉, 李婷婷, 沈朝华,等. 氮素形态对香榧苗期光合作用、主要元素吸收及氮代谢的影响[J]. 林业科学, 2014, 50(10):158-163. DOI: 10.11707/j.1001-7488.20141022.
TANG H, LI T T, SHEN C H, et al. Effects of nitrogen forms on foliar photosynthesis, nutrient status and nitrogen metabolism ofTorreya grandis seedlings[J]. Scientia Silvae Sinicae, 2014, 50(10):158-163.
[10] 樊卫国, 葛会敏. 不同形态及配比的氮肥对枳砧脐橙幼树生长及氮素吸收利用的影响[J]. 中国农业科学, 2015, 48(13):2666-2675. DOI: 10.3864/j.issn.0578-1752.2015.13.018.
FAN W G, GE H M. Effects of nitrogen fertilizer of different forms and ratios on the growth, nitrogen absorption and utilization of young navel orange trees grafted on Poncirus trifoliata[J]. Scientia Agricultura Sinica, 2015, 48(13):2666-2675.
[11] 苏芸芸, 王康才, 薛启. 氮素形态对藿香光合作用、氮代谢及品质的影响[J]. 南京农业大学学报, 2016, 39(4):543-549. DOI: 10.7685/jnau.201601014.
SU Y Y, WANG K C, XUE Q. Effects of nitrogen form on photosynthesis, nitrogen metabolism and quality of Agastache rugosa[J]. Journal of Nanjing Agricultural University, 2016, 39(4):543-549.
[12] PIWPUAN N, ZHAI X, BRIX H. Nitrogen nutrition of Cyperus laevigatus, and Phormium tenax: effects of ammonium versus nitrate on growth, nitrate reductase activity and N uptake kinetics[J]. Aquatic Botany, 2013, 106(4):42-51. DOI: 10.1016/j.aquabot.2013.01.002.
[13] 陈磊, 朱月林, 杨立飞,等. 氮素形态配比对菜用大豆生长及籽粒膨大中矿质营养含量的影响[J]. 西北农业学报, 2010, 19(10):189-193. DOI: 10.3969/j.issn.1004-1389.2010.10.038.
CHEN L, ZHU Y L, YANG L F, et al. Effects of nitrogen forms and ratios on carbon-nitrogen assimilation in developing seeds of vegetable soybean[J]. Acta Agriculturae Boreali-occidentalis Sinica, 2010, 19(10):189-193.
[14] CRUZ C, LIPS S H, MARTINS-LOUCAO M A. The effect of nitrogen source on photosynthesis of carob at high CO2 concentrations[J]. Physiologia Plantarum, 1993, 89(3):552-556. DOI:10.1111/j.1399-3054.1993.tb05212.x.
[15] 胡晓航, 周建朝, 陈立新,等. 铵态氮和氨基酸态氮配施对甜菜生长特性及碳代谢的影响[J]. 植物研究, 2015, 35(3):370-377. DOI: 10.7525/j.issn.1673-5102.2015.03.008.
HU X H, ZHOU J C, CHEN L X, et al. Effects of ammonium and amio-acid nitrogen fertilization on plant growth and carbon metabolism of sugar beets(Beta vulgaris L.)[J]. Bulletin of Botanical Research, 2015, 35(3):370-377.
[16] EVANS J R. Nitrogen and photosynthesis in the flag leaf of wheat(Triticum aestivum L.)[J]. Plant Physiology, 1983, 72(2):297. DOI: 10.2307/4268022.
[17] 李彩凤, 马凤呜, 赵越,等. 氮素形态对甜菜氮糖代谢关键酶活性及相关产物的影响[J]. 作物学报, 2003, 29(1):128-132. DOI: 10.3321/j.issn:0496-3490.2003.01.023.
LI C F, MA F M, ZHAO Y, et al. Effects of nitrogen forms on key enzyme activities and related products in sugar and nitrogen metabolism of sugar beet(Beta vulgaris L.)[J]. Acta Agronomica Sinica, 2003, 29(1):128-132.
[18] 曹福亮. 中国银杏志[M]. 北京:中国林业出版社, 2007.
[19] 国靖, 汪贵斌, 曹福亮. 施肥对银杏叶片光合作用及营养元素质量分数的影响[J]. 浙江农林大学学报, 2016, 33(6):969-975. DOI: 10.11833/j.issn.2095-0756.2016.06.007.
GUO J, WANG G B, CAO F L. Photosynthesis and nutrient content with fertilization for Ginkgo biloba leaves[J]. Journal of Zhejiang A & F University, 2016, 33(6):969-975.
[20] 郑军, 曹福亮, 郁万文. 不同氮素形态及配比对生育后期银杏叶品质的影响[J]. 浙江农林大学学报, 2007, 24(5):564-568. DOI: 10.3969/j.issn.2095-0756.2007.05.010.
ZHENG J, CAO F L, YU W W. Effects of different nitrogen forms and ratios on quality of Ginkgo biloba leaves at late growth stage[J]. Journal of Zhejiang A & F University, 2007, 24(5):564-568.
[21] 李合生. 植物生理生化实验原理和技术[M]. 北京:高等教育出版社, 2000.
[22] 宋建民, 田纪春. 植物光合碳和氮代谢之间的关系及其调节[J]. 植物生理学报, 1998, 34(3):230-238.
SONG J M, TIAN J C. Relationship between photosynthetic carbon and nitrogen metabolism in plants and its regulation[J]. Plant Physiology Communications, 1998, 34(3):230-238.
[23] 牛德新, 连文力, 崔红,等. 施氮量对烤烟成熟期中部烟叶碳氮代谢及相关基因表达的影响[J]. 烟草科技, 2017, 49(8):8-13.
ZHU D X, LIAN W L, CU H, et al. Effects of nitrogen application rate on carbon and nitrogen metabolisms and related gene expression in middle leaves of flue-cured tobacco at mature stage[J]. Tobacco Science & Technology, 2017, 49(8):8-13.
[24] WALCH-LIU P, FILLEUR S, GAN Y, et al. Signaling mechanisms integrating root and shoot responses to changes in the nitrogen supply[J]. Photosynthesis Research, 2005, 83(2):239-50. DOI: 10.1007/s11120-004-2080-9.
[25] 孙敏红, 卢晓鹏, 曹雄军,等. 不同氮素形态培养对枳橙幼苗硝酸还原酶活性及相关基因表达的影响[J]. 果树学报, 2017(4):410-417. DOI: CNKI:SUN:GSKK.0.2017-04-003.
SUN M H, LU X P, CAO X J, et al. Effect of different forms of nitrogen on the activity of nitrate reductase and expression of the relative genes in Citrus sinensis×Poncirus trifoliate[J]. Journal of Fruit Science, 2017(4):410-417.
[26] TSABARDUCAS, CHATZISTATHIS, THERIOS, et al. How nitrogen form and concentration affect growth, nutrient accumulation and photosynthetic performance of Olea europaea L.[J]. Scientia Horticulturae, 2017, 218:23-29. DOI: 10.1016/j.scienta.2017.02.012.
[27] 吕婷婷, 施晟璐, 唐晓清,等. 不同氮素形态和配比对菘蓝根的生长及含氮成分含量和总量的影响[J]. 植物资源与环境学报, 2016, 25(1):62-70. DOI: 10.3969/j.issn.1674-7895.2016.01.08.
LYU T T, SHI S L, TANG X Q, et al. Effect of nitrogen with different forms and ratios on growth,and content and total amount of nitrogen component in root of Isatis indigotica[J]. Journal of Plant Resources and Environment, 2016, 25(1):62-70.
[28] 陈胜勇, 李观康, 汪云,等. 谷氨酰胺合成酶的研究进展[J]. 中国农学通报, 2010, 26(22):45-49.
CHEN S Y, LI G K, WANG Y,et al. The research progress of glutamine synthetase[J]. Chinese Agricultural Science Bulletin, 2010, 26(22):45-49.

相似文献/References:

[1]汪贵斌,曹福亮,朱灿灿,等.宁化县银杏古树种核特征[J].南京林业大学学报(自然科学版),2009,33(02):022.[doi:10.3969/j.jssn.1000-2006.2009.02.006]
 WANG Gui bin,CAO Fu liang,ZHU Can can,et al.The nut characters of old Ginkgo trees in Ninghua county[J].Journal of Nanjing Forestry University(Natural Science Edition),2009,33(02):022.[doi:10.3969/j.jssn.1000-2006.2009.02.006]
[2]王芳,张静,邢世岩*,等.银杏品种枝桠管胞形态径向变化趋势研究[J].南京林业大学学报(自然科学版),2009,33(03):099.[doi:10.3969/j.jssn.1000-2006.2009.03.023]
 WANG Fang,ZHANG Jing,XING Shi yan*,et al.The radial variation trend of tracheid morphology of branches from Ginkgo biloba L.cultivars[J].Journal of Nanjing Forestry University(Natural Science Edition),2009,33(02):099.[doi:10.3969/j.jssn.1000-2006.2009.03.023]
[3]汪贵斌,曹福亮,程鹏,等.不同银杏复合经营模式土壤酶活性及综合评价[J].南京林业大学学报(自然科学版),2010,34(04):001.[doi:10.3969/j.jssn.1000-2006.2010.04.001]
 WANG Gui bin,CAO Fu liang,CHENA Peng,et al.The activities and integrated evaluation of soil enzymes of different agroforestry patterns of Ginkgo biloba L.[J].Journal of Nanjing Forestry University(Natural Science Edition),2010,34(02):001.[doi:10.3969/j.jssn.1000-2006.2010.04.001]
[4]景茂,曹福亮*,汪贵斌,等.土壤水分含量对银杏生长及生物量分配的影响[J].南京林业大学学报(自然科学版),2005,29(03):005.[doi:10.3969/j.jssn.1000-2006.2005.03.002]
 JING Mao,CAO Fu-liang*,WANG Gui-bin,et al.The Effects of Soil Water Stress on Growth and Biomass Allocation of Ginkgo biloba[J].Journal of Nanjing Forestry University(Natural Science Edition),2005,29(02):005.[doi:10.3969/j.jssn.1000-2006.2005.03.002]
[5]景茂,曹福亮*,汪贵斌,等.土壤7J~分含量对银杏光合特性的影响[J].南京林业大学学报(自然科学版),2005,29(04):083.[doi:10.3969/j.jssn.1000-2006.2005.04.020]
 JING MAO,CAO Fu-liang*,WANGGui-bin,et al.The Effects of Soil Water Contents on Photosynthetic Characteristics of Ginkgo[J].Journal of Nanjing Forestry University(Natural Science Edition),2005,29(02):083.[doi:10.3969/j.jssn.1000-2006.2005.04.020]
[6]曹福亮,黄敏仁,桂仁意,等.银杏主要栽培品种遗传多样性分析[J].南京林业大学学报(自然科学版),2005,29(06):001.[doi:10.3969/j.jssn.1000-2006.2005.06.001]
 CAO Fu-liang,HUANG Min-ren,GUI Ren-yi,et al.The Fingerprinting and Genetic Diversity of Main Ginkgo Cultivars[J].Journal of Nanjing Forestry University(Natural Science Edition),2005,29(02):001.[doi:10.3969/j.jssn.1000-2006.2005.06.001]
[7]汪贵斌,袁安全,曹福亮,等.土壤水分胁迫对银杏无机营养元素含量的影响[J].南京林业大学学报(自然科学版),2005,29(06):015.[doi:10.3969/j.jssn.1000-2006.2005.06.004]
 WANG Gui-bin,YUAN An-quan,CAO Fu-liang,et al.The Effects of Water Stress on Nutrient Element Contents in Root, Stem and Leaf of Ginkgo[J].Journal of Nanjing Forestry University(Natural Science Edition),2005,29(02):015.[doi:10.3969/j.jssn.1000-2006.2005.06.004]
[8]陈颖,曹福亮*.稀土对银杏组培芽苗生长及其不定根的影响[J].南京林业大学学报(自然科学版),2005,29(06):054.[doi:10.3969/j.jssn.1000-2006.2005.06.013]
 CHEN Ying,CAO Fu-liang*.Effects of Rare Earth on Growth of Cultured Terminal Buds and on Induction of Adventitious Roots of Ginkgo biloba In Vitro[J].Journal of Nanjing Forestry University(Natural Science Edition),2005,29(02):054.[doi:10.3969/j.jssn.1000-2006.2005.06.013]
[9]谢宝东,王华田.光质和光照时间对银杏叶片黄酮、内酯含量的影响[J].南京林业大学学报(自然科学版),2006,30(02):051.[doi:10.3969/j.jssn.1000-2006.2006.02.012]
 XIE Bao-dong,WANG Hua-tian.Effects of Light Spectrum and Photoperiod on Contents of Flavonoid and Terpene in Leaves of Ginkgo biloba L.[J].Journal of Nanjing Forestry University(Natural Science Edition),2006,30(02):051.[doi:10.3969/j.jssn.1000-2006.2006.02.012]
[10]彭方仁,王改萍,郭娟.银杏营养贮藏蛋白质的细胞学及生物化学分析[J].南京林业大学学报(自然科学版),2006,30(04):109.[doi:10.3969/j.jssn.1000-2006.2006.04.026]
 PENG Fang-ren,WANG Gai-ping,GUO Juan.Analyses on the Cytological and Biochemical of Vegetative Storage Protein in Ginkgo biloba[J].Journal of Nanjing Forestry University(Natural Science Edition),2006,30(02):109.[doi:10.3969/j.jssn.1000-2006.2006.04.026]

备注/Memo

备注/Memo:
收稿日期:2018-07-19 修回日期:2018-12-11
基金项目:国家重点研发计划(2017YFD0600700)。
第一作者:黄东静(291051451@qq.com)。
*通信作者:汪贵斌(gbwang@njfu.com.cn),教授,ORCID(0000-0002-9987-6496)。
更新日期/Last Update: 2019-03-30