[1]韩言利,郭起荣*,韩永华*,等.毛竹和厚壁毛竹的核型分析及45S和5S rDNA的FISH分析[J].南京林业大学学报(自然科学版),2019,43(02):203-208.[doi:10.3969/j.issn.1000-2006.201802013]
 HAN Yanli,GUO Qirong*,HAN Yonghua*,et al.The karyotype and FISH analysis of 45S and 5S rDNA on the chromosomes of Phyllostachys edulis and Phyllostachys edulis ‘Pachyloen'[J].Journal of Nanjing Forestry University(Natural Science Edition),2019,43(02):203-208.[doi:10.3969/j.issn.1000-2006.201802013]
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毛竹和厚壁毛竹的核型分析及45S和5S rDNA的FISH分析
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《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

卷:
43
期数:
2019年02期
页码:
203-208
栏目:
研究简报
出版日期:
2019-03-30

文章信息/Info

Title:
The karyotype and FISH analysis of 45S and 5S rDNA on the chromosomes of Phyllostachys edulis and Phyllostachys edulis ‘Pachyloen'
文章编号:
1000-2006(2019)02-0203-06
作者:
韩言利1郭起荣2*韩永华1*于钊妍2米月颖2
(1. 江苏师范大学,生命科学学院,江苏省系统发育与比较基因组学重点实验室,江苏 徐州 221116; 2. 南京林业大学,林学院,南方现代林业协同创新中心,江苏 南京 210037)
Author(s):
HAN Yanli1GUO Qirong2* HAN Yonghua1* YU ZhaoyuanMI Yueyin
(1. Jiangsu Key Laboratory of Phylogenomics and Comparative Genomics, School of Life Sciences,Jiangsu Normal University, Xuzhou 221116, China; 2. Co-innovation Center for the Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037, China)
关键词:
毛竹 厚壁毛竹 核型分析 rDNA 荧光原位杂交
Keywords:
Phyllostachys edulis Phyllostachys edulis ‘Pachyloen' karyotype analysis rDNA FISH(fhcorescence insitu hybridization)
分类号:
S795.9; Q343.2
DOI:
10.3969/j.issn.1000-2006.201802013
文献标志码:
A
摘要:
【目的】厚壁毛竹(Phyllostachys edulis ‘Pachyloen')是毛竹(Phyllostachys edulis)的栽培品种,是具有较高经济价值的优特种质。对毛竹与厚壁毛竹进行细胞遗传学研究,了解其核型特征。【方法】采用双色荧光原位杂交技术,对毛竹、厚壁毛竹进行了核型分析,利用45S 和 5S rDNA 在其染色体上进行了物理定位。【结果】毛竹、厚壁毛竹的染色体数目都为48条,毛竹的染色体相对组成为2n=48=12L+10M2+14M1+12S,核型公式为 2n=48=32m+12sm+4st(2SAT); 厚壁毛竹的染色体相对组成为2n=48=12L+8M2+16M1+12S,核型公式为2n=48=34m+10sm+4st(2SAT)。毛竹与厚壁毛竹的染色体不对称系数分别为61.41%和59.55%,均属于“2B”型。荧光原位杂交(FISH)结果表明:毛竹、厚壁毛竹都有2个45S rDNA位点和4个5S rDNA位点。【结论】厚壁毛竹的染色体数量为48条,补充了毛竹的染色体为48条的循证依据; 获得了毛竹、厚壁毛竹45S 和 5S rDNA荧光原位杂交核型,两者的45S和5S rDNA 位点没有明显差异,具有相似的核型,显示其较近的亲缘关系。
Abstract:
【Objective】Phyllostachys edulis ‘Pachyloen' is a cultivar of Phyllostachys edulis, which is an economically-important germplasm. Cytogenetic research on this germplasm provide a cytomolecular basis for genetic research on bamboo species.【Method】Karyotype analysis and physical localization of 45S and 5S rDNA on the chromosomes of Ph. edulis and Ph. edulis ‘Pachyloen' were performed by double color fluorescence in situ hybridization(FISH).【Result】The results of karyotype analysis indicated that the chromosome number in the two materials was 2n=48, the karyotype formula of Ph. edulis and Ph. edulis ‘Pachyloen' was 2n=48=32m+12sm+4st(2SAT)and 2n=48=34m+10sm+4st(2SAT), respectively. The chromosome composition of relative length was 2n=48=12L+10M2+14M1+12S and 2n=48=12L+8M2+16M1+12S, respectively. The asymmetrical karyotype coefficients of Ph. edulis and Ph. edulis ‘Pachyloen' were 61.41% and 59.55%, and their karyotypes were both of 2B type. The results showed that there were 2 loci of 45S rDNA and 4 loci of 5S rDNA in both Ph. edulis and Ph. edulis ‘Pachyloen'. 【Conclusion】The chromosome number in the two materials is 2n=48, the 45S and 5S rDNA loci of the two materials have no obvious differences, and the two varieties have similar karyotypes, which shows that they have close genetic relatedness.

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备注/Memo

备注/Memo:
收稿日期:2018-02-10 修回日期:2018-09-16
基金项目:江苏人才基金(GXL2018001); 江苏省农业科技自主创新资金项目CX(16)1005)。
第一作者:韩言利(840643974@qq.com)。
*通信作者:郭起荣(QRGUO@hotmail.com),教授,负责试验设计与文章修改,ORCID(0000-0002-1280-3455); 韩永华(hanyonghua@jsnu.edu.cn),教授,负责试验技术与分析,ORCID(0000-0001-9305-2536)。
更新日期/Last Update: 2019-03-30