[1]赵 慧,莫家兴,华 慧,等.柳杉花粉母细胞减数分裂进程及异常行为[J].南京林业大学学报(自然科学版),2019,43(03):045-50.[doi:10.3969/j.issn.1000-2006.201809001]
 ZHAO Hui,MO Jiaxing,HUA Hui,et al.Meiosis process and abnormal behavior of pollen mother cells in Cryptomeria fortunei[J].Journal of Nanjing Forestry University(Natural Science Edition),2019,43(03):045-50.[doi:10.3969/j.issn.1000-2006.201809001]
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柳杉花粉母细胞减数分裂进程及异常行为
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《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

卷:
43
期数:
2019年03期
页码:
045-50
栏目:
研究论文
出版日期:
2019-05-15

文章信息/Info

Title:
Meiosis process and abnormal behavior of pollen mother cells in Cryptomeria fortunei
文章编号:
1000-2006(2019)03-0045-06
作者:
赵 慧莫家兴华 慧郭臻昊徐 进*
南京林业大学,南方现代林业协同创新中心,南京林业大学林学院,江苏 南京 210037
Author(s):
ZHAO HuiMO JiaxingHUA HuiGUO Zhenhao XU Jin*
Co-Innovation Center for the Sustainable Forestry in Southern China,College of Forestry, Nanjing Forestry University, Nanjing 210037, China
关键词:
柳杉 雄球花 花粉母细胞 减数分裂
Keywords:
Cryptomeria fortunei the male strobilus pollen mother cell meiosis
分类号:
S792.11; Q23
DOI:
10.3969/j.issn.1000-2006.201809001
文献标志码:
A
摘要:
【目的】观察柳杉花粉母细胞减数分裂进程及染色体的变化和异常行为,为进一步开展柳杉的生殖生物学与分子细胞遗传学研究提供参考。【方法】以柳杉的雄球花(小孢子叶球)为材料,采用压片法研究柳杉花粉母细胞减数分裂的变化规律。【结果】柳杉花粉母细胞减数分裂的胞质分裂类型为同时型,其进程一般开始于10月中下旬并持续到11月上旬。观察发现柳杉雄球花外观颜色的变化与减数分裂进程有关,同一侧枝上柳杉雄球花的发育不一致,同一花序中的不同小孢子叶球的花粉母细胞减数分裂进程也不同步。通过观察柳杉的花粉母细胞减数分裂过程,发现存在单价染色体、不等价二价体、滞后染色体、分裂不同步、不均等分裂、异常四分体等异常现象。【结论】柳杉雄球花外观颜色的变化与减数分裂进程密切相关,其减数分裂过程中染色体存在异常现象。
Abstract:
【Objective】To provide a reference for the reproduction biology and molecular cytogenetics research of Cryptomeria fortunei, we studied the meiosis process of the pollen mother cells, including the transformation and abnormal behavior of the chromosomes. 【Method】Taking the male strobilus of C. fortunei as experimental material, the variation in the meiosis of the pollen mother cells of C. fortunei was studied using carbol fuchsin staining. 【Result】The cytokinesis of the pollen mother cells of C. fortunei was the simultaneous type, with the process generally starting in mid-late October and lasting until early November. The change in the color of the male strobilus was related to the process of meiosis. Additionally, the development of different staminate male strobilus on the same lateral branch was inconsistent and the meiosis of different staminate strobilus on the same inflorescence was not synchronous. Abnormal phenomena were observed, such as monochromatic chromosomes, unequal bivalents, lagging chromosomes, unsynchronized division, inhomogeneous division, abnormal tetrads, and so on. 【Conclusion】The change in the color of the male strobilus of C. fortunei was closely related to the meiosis process. Additionally, abnormal phenomena of the chromosomes exist during meiosis. These results can provide a theoretical basis for the cytological study of staminate strobilus.

参考文献/References:

[1] 中国科学院中国植物志委员会. 中国植物志:7卷[M]. 北京:科学出版社, 1978:293-295.
Chinese Flora Committee of the Chinese Academy of Sciences. Chinese flora: Vol. Ⅶ [M]. Beijing: Science Press, 1978:293-295.
[2] 许淑芬. 四川柳杉扦插繁育技术研究[D].四川:四川农业大学, 2017.
XU S F. Study on the Cryptomeria D.Don cutting and seedling technique[D].Sichuan: Sichuan Agricultural University, 2017.
[3] 陈岳武,贾 稝,康亦强,等. 福建柳杉小群体遗传变异的研究[J].南京林业大学学报(自然科学版), 1982, 6(3): 74-83. DOI:10.3969/j.jssn.1000-2006.1982.03.006.
CHEN Y W,JIA P, KANG Y Q, et al. A study of the inheritance and variation of the small populations of Cryptomeria fortunei in Fujian Province[J]. Journal of Nanjing Forestry University(Natural Sciences Edition), 1982, 6(3):74-83.
[4] 祝晨辰,徐进,欧阳磊,等. 柳杉优良无性系离体培养再生体系研究[J]. 南京林业大学学报(自然科学版), 2015, 39(3): 55-58. DOI:10.3969/j.issn.1000-2006.2015.03.011.
ZHU C C, XU J, OUYANG L, et al. Study on regeneration system of Cryptomeria clones in vitro culture[J]. Journal of Nanjing Forestry University(Natural Sciences Edition), 2015,39(3): 55-58.
[5] 林祖建. 柳杉初级种子园31年生全同胞子代评价与选择研究[J]. 林业勘察设计, 2016, 36(1): 42-46.
LIN Z J. Evaluation and selection of 31- year-old full-sib progeny in primary seed orchard of Cryptomeria fortunei[J]. Forestry Investigation and Design, 2016, 36(1): 42-46.
[6] LIU H E,WU X M. Embryogenesis of Cryptomeria fortunei[J]. Journal of Integrative Plant Biology, 1986, 28(3): 256-262.
[7] 钱莲芳. 柳杉花芽分化的观察[J]. 浙江林学院学报, 1988, 5(2): 62-68.
QIAN L F. Observation on flower bud differentiation of Cryptomeria[J]. Journal of Zhejiang Forestry College, 1988, 5(2): 173-179.
[8] 徐进,施季森,王桂凤. 杉木雄球花减数分裂及其进程[J]. 南京林业大学学报(自然科学版), 2007, 31(6): 6-10. DOI: 10.3969/j.issn.1000-2006.2007.06.002.
XU J, SHI J S, WANG G F. Meiosis of male strobilus in Chinese fir[J]. Journal of Nanjing Forestry University(Natural Sciences Edition), 2007, 31(6): 6-10.
[9] 辛昊阳,王帅,刘光欣,等. 美洲黑杨小孢子母细胞减数分裂进程与花芽及花药外观形态相关性研究[J]. 南京林业大学学报(自然科学版), 2016, 40(2): 48-52. DOI: 10.3969/j.issn.1000-2006.2016.02.008
XIN H Y, WANG S, LIU G X, et al. Relationship between the meiosis processes of microsporocytes and morphology of male flower buds and anthers in Populus deltoides Marsh[J]. Journal of Nanjing Forestry University(Natural Sciences Edition), 2016, 40(2): 48-52.
[10] 兰月,辛昊阳,赵乙琏,等. 小叶杨花粉母细胞减数分裂及花粉萌发力观测[J]. 南京林业大学学报(自然科学版), 2017, 41(6): 177-180. DOI:10.3969/j.issn.1000-2006.201704060.
LAN Y, XIN H Y, ZHAO Y L, et al. Study on meiosis of microspore mother cells and germination rate of pollen grains in Populus simonii Carr[J].Journal of Nanjing Forestry University(Natural Sciences Edition), 2017, 41(6): 177-180.
[11] 朴锦,具红光,朴钟云. 关苍术花粉母细胞减数分裂及其雄配子体发育研究[J]. 西北植物学报, 2015, 35(8): 1547-1553. DOI: 10.7606/j.issn.1000-4025.2015.08.1547.
PU J, JU H G, PU Z Y. Pollen mother cell meiosis and male gametophyte development of Atractylodes japonica[J]. Acta Botanica Boreali-Occidentalia Sinica, 2015, 35(8): 1547-1553.
[12] 高爱新,秦国峰,王培蒂,等. 马尾松雄球花生长发育特性及花粉采收特点[J]. 林业科学研究, 2007, 20(3): 437-441. DOI: 10.3321/j.issn:1001-1498.2007.03.026.
GAO A X, QIN G F, WANG P D, et al. A study of growth development of male strobilus and pollen collection of Pinus massoniana Lamb. [J]. Forest Research, 2007, 20(3): 437-441.
[13] HE Z, LI J, CAI Q, et al. Cytogenetic studies on Metasequoia glyptostroboides, a living fossil species[J]. Genetica, 2004, 122(3): 269-276. DOI: 10.1007/s10709-004-0926-x.
[14] 张守攻,韩素英,杨文华,等. 红皮云杉花粉母细胞减数分裂和花粉发育的研究[J]. 园艺学报, 2009, 36(7): 1023-1030. DOI: 10.3321/j.issn:0513-353X.2009.07.012
ZHANG S G, HAN S Y, YAG W H, et al. Studies on the meiosis of pollen mother cell and pollen development of Picea koraiensis Nakai[J]. Acta Horticulturae Sinica, 2009, 36(7): 1023-1030.
[15] 邓辉胜,张大明. 3种云杉小孢子母细胞减数分裂研究[J]. 河池学院学报, 2005, 25(5): 23-27. DOI: 10.3969/j.issn.1672-9021.2005.05.006
DENG H S,ZHANG D M. Microsporogenesis and meiotic behavior in three species of the genus Picea(Pinaceae)[J]. Journal of Hechi University, 2005, 25(5): 23-27.
[16] HOSOO Y. Development of pollen and female gametophytes in Cryptomeria japonica[J]. International Journal of Plant Developmental Biology, 2007, 1(1), 116-121. DOI: 10.1016/S0140-6736(02)11679-5.
[17] 陈欣,杨倩,张平冬,等. 胡杨小孢子母细胞减数分裂与花粉变异研究[J]. 西北林学院学报, 2015, 30(2): 90-95. DOI: 10.3969/j.issn.1001-7461.2015.02.15
CHEN X, YANG Q, ZHANG P D, et al. Studies on meiosis of microspore mother cell and variations of pollen grains in Populus enphratica[J]. Journal of Northwest Forestry University, 2015, 30(2): 90-95.
[18] 刘丽琴,石胜友,李伟才,等. 荔枝花粉母细胞减数分裂观察[J]. 果树学报, 2015, 32(2): 254-258. DOI: 10.13925/j.cnki.gsxb.20140308.
LIU L Q, SHI Y S, LI W C, et al. Meiotic observations of the pollen mother cells of Litchi chinenisis Sonn[J]. Journal of Fruit Science, 2015, 32(2): 254-258.
[19] 许重阳,王君,叶粟,等. 白杨杂种三倍体小孢子母细胞减数分裂及花粉形态观察[J]. 西北植物学报, 2011, 31(12): 2454-2458.
XU C Y, WANG J, YE L, et al. Meiosis of microsporocyte and variation of pollen in an allotriploid white poplar[J]. Acta Botanica Boreali-Occidentalia Sinica, 2011, 31(12): 2454-2458.
[20] 刘春霞,何群燕,金危危. 植物减数分裂中的染色体配对、联会和重组研究进展[J]. 遗传, 2010, 32(12): 1223-1231. DOI: 10.3724/SP.J.1005.2010.01223.
LIU C X, HE Q Y, JIN W W. The pairing, synapsis and recombination of meiosis in plant[J]. Hereditas, 2010, 32(12): 1223-1231.
[21] ZHANG Z, KANG X. Cytological characteristics of numerically unreduced pollen production in Populus tomentosa Carr[J]. Euphytica, 2010, 173(2): 151-159. DOI: 10.1007/s10681-009-0051-0.
[22] 张平冬,康向阳. 胡杨小孢子发生及微管骨架变化与异常研究[J]. 西北植物学报, 2013, 33(11): 2166-2171.
ZHAG P D, KANG X Y. Organization of microtubule and its abnormities during microsporogenesis in Populus enphratica[J]. Acta Botanica Boreali-Occidentalia Sinica, 2013, 33(11): 2166-2171.
[23] 陆彦,王頔,张敏,等. 银杏小孢子和花粉发育过程的超微结构观察[J]. 电子显微学报, 2011, 30(3): 265-273. DOI: 10.3969/j.issn.1000-6281.2011.03.018
LU Y, WANG D, ZHANG M, et al. Ultrastructural observation of the microsporogenesis and pollen development in Ginkgo biloba L.[J].Journal of Chinese Electron Microscopy Society, 2011, 30(3): 265-273.
[24] 刘望舒,韩林芝,朱嵊,等. 杨树雄性不育品种花粉发育过程的细胞学观察[J]. 南京林业大学学报(自然科学版), 2019, 43(1):198-203. DOI:10.3969/j.issn.1000-2006.201805034.
LIU W S, HAN Z L, ZHU S,et al. Cytological observation on pollen development of the male sterile poplar variety[J]. Journal of Nanjing Forestry University(Natural Sciences Edition), 2019, 43(1):198-203.
[25] TSUBOMURA M, KURITA M, WATANABE A. Determination of male strobilus developmental stages by cytological and gene expression analyses in Japanese cedar(Cryptomeria japonica)[J]. Tree Physiology, 2016, 36(5): 653-666. DOI: 10.1093/treephys/tpw001.

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备注/Memo

备注/Memo:
收稿日期:2018-09-03 修回日期:2019-02-20
基金项目:国家林业公益性行业科研专项项目(201304104); 江苏高校优势学科建设工程资助项目(PAPD)。
第一作者:赵慧(1686924145@qq.com)。*通信作者:徐进(xjinhsh@njfu.edu.cn),教授,ORCID(0000-0001-8224-5957)。
更新日期/Last Update: 2019-05-15