[1]李 丽,MANNING William,王效科.大气臭氧浓度升高对樱桃萝卜生长和生物量的影响[J].南京林业大学学报(自然科学版),2019,43(05):187-192.[doi:10.3969/j.issn.1000-2006.201809011]
 LI Li,MANNING William,WANG Xiaoke.Effects of elevated atmospheric ozone on the growth and biomass of Raphanus sativus L.[J].Journal of Nanjing Forestry University(Natural Science Edition),2019,43(05):187-192.[doi:10.3969/j.issn.1000-2006.201809011]





Effects of elevated atmospheric ozone on the growth and biomass of Raphanus sativus L.
李 丽12MANNING William3王效科2
(1.南京林业大学竹类研究所,江苏 南京 210037; 2.中国科学院生态环境研究中心,城市与区域生态国家重点实验室, 北京 100085; 3.美国麻省大学阿姆赫斯特分校农业科学学院,马萨诸塞州 阿姆赫斯特 01003)
LI Li12 MANNING William3 WANG Xiaoke2
(1.Bamboo Research Institute,Nanjing Forestry University, Nanjing 210037, China; 2. State Key Laboratory of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; 3. Stockbridge School of Agriculture, University of Massachusetts, Amherst MA 01003, USA)
大气臭氧 AOT40 樱桃萝卜 肉质根 生物量 受害症状
atmospheric ozone(O3) AOT40 Raphanus sativus L. hypocotyl biomass injury symptom
【目的】近地层臭氧(O3)是具有强氧化性的二次污染物,可损伤植物内部结构和生理功能,造成作物生长量和品质下降。了解不同大气O3浓度下樱桃萝卜各生长时期的叶片受害和生物量状况,分析其受害症状、生物量与O3暴露剂量之间的相关关系,为探讨O3浓度升高对农作物产量的影响及作物大气O3标准的制定提供参考。【方法】利用12个生长室设置了4个大气臭氧(O3)浓度(30、50、70和90 nmol/mol),对樱桃萝卜叶片的受害症状和第7、14、21和28天的生长和生物量变化进行了研究,并拟合计算了O3暴露剂量AOT40和叶片受害症状、生物量变化间的相关关系。【结果】① 70和90 nmol/mol O3处理下,第7天观测发现樱桃萝卜子叶上部出现褪绿斑点, 整叶局部薄厚不均,叶片受害症状随着O3熏蒸时间的增加而加重,出现O3受害症状的叶片比例和O3暴露剂量AOT40极显著相关(P<0.01)。②处理第21天时观测发现,O3熏蒸均对樱桃萝卜整体干质量产生影响; 与30 nmol/mol处理的相比,第28天时观察发现,50、70和90 nmol/mol O3处理后的樱桃萝卜肉质根干质量分别下降了45.1%、57.3% 和79.9%。干质量下降的幅度大于体积的,肉质根体积和干物质量都与O3暴露剂量AOT40线性相关。【结论】樱桃萝卜的生长和生物量受到O3浓度和累积时间两个因素共同影响,O3浓度升高显著抑制了樱桃萝卜生长,造成叶片受损,可食用肉质根体积和总干物质量显著下降。
【Objective】 High levels of atmospheric ozone(O3), which is an important secondary air pollutant with strong oxidizing capability, can reduce the quantity and quality of vegetation production by damaging the functions and physical structure of plant cells. Therefore, in order to establish a basic understanding of the effects of O3 on crop production, the present study investigated the effects of different O3 concentrations, including AOT40, on the growth(hypocotyl length), biomass(dry weight)and leaf health of cherry reddish (Raphanus sativus). 【Method】 Raphanus sativus were grown in growth chambers under four different O3 concentrations(30, 50, 70 and 90 nmol/mol), and the growth, biomass and foliar injury status of the plants were assessed on a weekly basis for 4 weeks. 【Result】 ① The O3 injury symptoms that were observed under 70 and 90 nmol/mol(i.e., cotyledons with chlorotic stippling and inconsistent thickness)developed within the first week of O3 fumigation, and the injury symptoms intensified with the duration of treatment. Furthermore, the proportion of leaves with injury symptoms was positively correlated with AOT40(accumulated O3 exposure over a threshold of 40(nmol/mol)(P<0.01). ② Meanwhile, elevated O3 concentrations significantly reduced dry mass and hypocotyl size since the 3rd week. At the end of 4th week, the dry mass of hypocotyl had been reduced by 45.1%, 57.3% and 79.9% in 50, 70 and 90 nmol/mol O3 treatments, respectively, when compared to the control group. In addition, the reduction of hypocotyl exhibited a linear relationship with AOT40, and dry mass declined more rapidly than hypocotyl size.【Conclusion】The growth, biomass and injury symptoms of Raphanus sativus were affected by both O3 concentration and duration of exposure.


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收稿日期:2018-09-12 修回日期:2019-04-10 基金项目:国家自然科学基金青年基金项目(31700439); 中国博士后基金面上项目(2018M631595); 国家重点研发计划(2017YFE0127700)。 第一作者:李丽(lili7381741@yeah.net),博士,ORCID(0000-0003-0077-114X)。 引文格式:李丽,MANNING William,王效科. 大气臭氧浓度升高对樱桃萝卜生长和生物量的影响[J]. 南京林业大学学报(自然科学版),2019,43(5):187-192.
更新日期/Last Update: 2019-10-08