[1]徐 清,闭鸿雁,崔光帅,等.珍稀濒危植物毛果木莲幼苗光合特性及对遮阴处理的响应[J].南京林业大学学报(自然科学版),2019,43(06):046-52.
 XU Qing,BI Hongyan,CUI Guangshuai,et al.Response of photosynthetic fluorescence of the endangered plant Manglietia ventii seedlings to shade treatment[J].Journal of Nanjing Forestry University(Natural Science Edition),2019,43(06):046-52.
点击复制

珍稀濒危植物毛果木莲幼苗光合特性及对遮阴处理的响应
分享到:

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

卷:
43
期数:
2019年06期
页码:
046-52
栏目:
研究论文
出版日期:
2019-11-25

文章信息/Info

Title:
Response of photosynthetic fluorescence of the endangered plant Manglietia ventii seedlings to shade treatment
文章编号:
1000-2006(2019)06-0046-07
作者:
徐 清1闭鸿雁1崔光帅1郭晓荣1周 睿1苏文华 1欧阳志勤2张光飞1 *
(1.云南大学生态学与地植物学研究所,云南 昆明 650091; 2. 云南省环境科学研究院,云南 昆明 650034)
Author(s):
XU Qing1 BI Hongyan1 CUI Guangshuai1 GUO Xiaorong1 ZHOU Rui1 SU Wenhua1 OUYANG Zhiqin2 ZHANG Guangfei1 䥺Symbolj@@
(1.Institute of Ecology and Geobotany, Yunnan University, Kunming 650091, China; 2. Yunnan Institute of Environmental Science, Kunming 650034, China)
关键词:
毛果木莲 遮阴 光合作用 蒸腾作用 叶绿素荧光参数
Keywords:
Manglietia ventii shade photosynthesis transpiration chlorophyll fluorescence parameter
分类号:
S718; Q945
摘要:
【目的】光是植物生长发育过程中重要的环境因子,可通过光敏色素等途径来调节植物的生长、发育。毛果木莲(Manglietia ventii)为中国特有种,是国家Ⅱ级重点保护植物。笔者采取人工遮阴方式,对毛果木莲幼苗的光合及叶绿素荧光参数等指标进行测定,探讨毛果木莲幼苗对光环境变化的生理响应和适应性,为开展其保护生物学研究提供理论依据,同时为濒危植物的保护及繁衍提供科学指导。【方法】利用黑色遮阴网进行人工遮阴处理,使用照度计反复测量以准确建立相对光照率(RI)分别为8%、30%和100%(全光照)的生境。待毛果木莲幼苗在3种光照生境中生长6个月后,应用Li-6400 XT型便携式光合-荧光测定仪(Licor-6400, Lincoln, USA),随机选取不同遮阴条件下日照充足的连体叶(每个生境梯度5株,每株2叶片,共30个重复,取其平均值)。于8:30—11:30进行光合-光响应曲线的测定。日变化前,暗适应30 min后进行3种生境下幼苗的PSⅡ最大光化学效率(Fv/ Fm)测定。天气晴朗时连续3 d,从7:00—19:00每隔2 h进行日变化的测定,测定净光合速率(Pn)、蒸腾速率(Tr)、气孔导度(Gs)、光合有效辐射(PAR)等光合参数; 光化学淬灭系数(qP)、非光化学淬灭系数(qN)、PS Ⅱ有效光化学量子产量(Fv'/Fm')、电子传递速率(ETR)等荧光参数。【结果】在限定条件下毛果木莲幼苗Pn对PAR的响应过程在不同遮阴程度下大致相同,且毛果木莲幼苗最大净光合速率(Pn, max)、光饱和点(PLSP)和光补偿点(PLCP)随遮阴程度的增大而降低,表明毛果木莲幼苗具有一定的耐阴性,能够通过降低Pn, maxPLSPPLCP来增强对弱光的利用能力。光合速率日变化呈现单峰型曲线,PnTrGs均随遮阴程度增大而降低,且均无“光合午休”现象,说明毛果木莲幼苗在水分充足的条件下能适应多光环境。qNFv'/Fm'和ETR日变化皆呈现单峰型曲线,均随遮阴程度增大而降低; 但qP的日变化随遮阴程度的增大而增加,说明遮阴促进光合作用与增强光能利用率和刺激叶肉细胞的光化学活性有关,主要通过增加PSⅡ反应中心的开放比例和降热耗散比例等途径适应弱光生境。而Fv/Fm随遮阴程度增加而增大,且RI为100%生境下其值低于正常值,具有强光抑制现象。说明长时间强光处理导致毛果木莲幼苗的光合机构受损,光化学活性降低。【结论】RI为100%时Fv/Fm显著低于其他两个遮阴处理(RI为30%、8%)(P<0.05, F=18.979, df=2),具有强光抑制现象,适当遮阴更利于毛果木莲幼苗生长,以RI为30%生境光强最佳。
Abstract:
【Objective】Light is an important environmental factor in the growth and development of plants. It effects plant growth and development by regulating the biosynthesis of phytochrome and other metabolic pathways. Manglietia ventii, is an endemic plant distributed in western China, and listed in the second-class national list of key conservative plants. The seedlings of M. ventii were cultivated under different light levels. The photosynthetic characteristics and chlorophyll fluorescence parameters of the seedlings were measured to investigate the adaptation and physiological responses and photo-protective mechanisms to the different light environment. Results may provide insights into the conservation and propagation of the endangered species. 【Method】Ten seedlings with similar size were randomly selected into 3 groups. Then each group was cultivated in the following relative illumination rate(RI)levels(100%, 30% and 8% created with different layers of black shading net)respectively for 6 months. During the growth period, the seedlings were watered and provided with nutrients properly. The photosynthetic characteristics of 5 healthy seedlings randomly chosen from RI 100%, 30% and 8%, respectively, were measured with the Li-6400 XT portable photosynthetic-fluorescence analyzer(Licor-6400, Lincoln, USA). The photosynthesis value of each seedling was recorded by averaging the data from 2 matured and healthy leaves. The photosynthetic-photoresponse curve was measured at 8:30-11:30 am Before the diurnal variation, the maximum photochemical efficiency(Fv/Fm)of the PSⅡ photosystem Ⅱ(PSⅡ)of the seedlings under the three light conditions were measured after dark adaptation for 30 minutes. The diurnal variation of photosynthetic parameters, including net photosynthetic rate(Pn), transpiration rate(Tr), stomatal conductance(Gs)were measured from 7:00 am to 19:00, for 3 days without clouds. At the same time, chlorophyll fluorescence parameters were measured, including photochemical quenching coefficient(qP)and non-photochemical quenching coefficient(qN), The effective photochemical quantum yield(Fv'/Fm')and electron transport rate(ETR)were measured with the same leaves for the measurement of photosynthesis. 【Result】Results indicated the response of Pn of seedlings under different levels of shade to photosynthetic active radiation(PAR)is similar; however, maximum net photosynthetic rate(Pn, max), light saturation point(PLSP)and light compensation point(PLCP)decreased with the increase in the levels of shade levels, which implicated that seedlings of M. ventii can tolerate shady environment. The diurnal variation of photosynthetic rate of all the seedlings under different light levels showed single peak curves, and Pn, Tr and Gs decreased with the increase in levels of shade. There is no midday depression of photosynthesis, which showed the seedlings of M. ventii can adapt to the light variation under the growth condition of sufficient water and nutrients. The diurnal variation of qN, Fv'/Fm', and ETR decreased with the increase in shading levels and showed single peak curves too. However, the diurnal variation of qP increased with the increase in shading levels, which indicated that shading promoted photosynthesis and enhanced the utilization of light energy and stimulated the photochemical activity of mesophyll cells. Leaves may mainly adapt to the low light habitat by increasing the open ratio of PSⅡ reaction center and the proportion of heat dissipation. Meanwhile, Fv/Fm was raised with the decrease of light levels. The value of Fv/Fm of leaves exposed to RI 100% is lower than 0.8(that is normal for most plants in nonstress condition), which showed the occurrence of strong light inhibition. It implicated that long-term strong light treatment could damage the photosynthetic components of M. ventii seedlings and reduce its photochemical activity. 【Conclusion】Fv/Fm in full light(RI 100%)was significantly lower than that in the shading treatments(RI 30%, RI 8%)(P<0.05, F = 18.979, df = 2), which illustrated that the shading treatments effectively weakened light suppression of the M. ventii seedlings. Conclusively, shading treatment is more favorable to the growth of M. ventii seedlings and the best growth of M. ventii seedlings was expected under RI 30%.

参考文献/References:

[1] ROZENDAAL D M A, HURTADO V H, POORTER L. Plasticity in leaf traits of 38 tropical tree species in response to light; relationships with light demand and adult stature[J]. Functional Ecology, 2006, 20(2): 207-216. DOI:10.1111/j.1365-2435.2006.01105.x. [2] 宋洋, 廖亮, 刘涛, . 不同遮荫水平下香榧苗期光合作用及氮分配的响应机制[J]. 林业科学, 2016, 52(5): 55-63. DOI:10.11707/j.1001-7488.20160507. SONG Y, LIAO L, LIU T, et al. Response of photosynthesis and nitrogen distribution of Torreya grandis ‘Merrilli' seedlings in different light regimes[J]. Scientia Silvae Sinicae, 2016, 52(5): 55-63. [3] 殷东生, 沈海龙. 森林植物耐荫性及其形态和生理适应性研究进展[J]. 应用生态学报, 2016, 27(8): 2687-2698. DOI:10.13287/j.1001-9332.201608.018. YIN D S, SHEN H L. Shade tolerance and the adaptability of forest plants in morphology and physiology:a review[J]. Chinese Journal of Applied Ecology, 2016, 27(8): 2687-2698. [4] 张勇, 程怡, 王清明, . 遮荫对月季光合特性及生长发育的影响[J]. 西北植物学报, 2014, 34(1): 162-168. DOI:10.7606/j.issn.1000-4025.2014.01.0162. ZHANG Y, CHENG Y, WANG Q M, et al. Effects of shading on growth and photosynthetic characteristics of Rosa hybrida[J]. Acta Botanica Boreali-Occidentalia Sinica, 2014, 34(1): 162-168. [5] 冯玉龙, 曹坤芳, 冯志立, . 四种热带雨林树种幼苗比叶重, 光合特性和暗呼吸对生长光环境的适应[J]. 生态学报, 2002, 22(6): 901-910. DOI:10.3321/j.issn:1000-0933.2002.06.015. FENG Y L,CAO K F, FENG Z L, et al. Acclimation of lamina mass per unit area, photosynthetic characteristics and dark respiration to growth light regimes in four tropical rainforest species[J]. Acta Ecologica Sinica, 2002, 22(6): 901-910. [6] 杨露, 于晓跃, 刘煜光, . 遮荫对2种彩叶风箱果叶色及光合特性的影响[J]. 河北农业大学学报, 2016, 39(5): 75-81. DOI:10.13320/j.cnki.jauh.2016.0111. YANG L, YU X Y, LIU Y G, et al. The influence of shading on leaf color and photosynthetic characteristics in two color-leafed plants of Physocarpus opulifolius[J]. Journal of Hebei Agricultural University, 2016, 39(5): 75-81. [7] 张旺峰, 樊大勇, 谢宗强, . 濒危植物银杉幼树对生长光强的季节性光合响应[J]. 生物多样性, 2005, 13(5): 387-397. DOI:10.3321/j.issn:1005-0094.2005.05.003. ZHANG W F, FAN D Y, XIE Z Q, et al. The seasonal photosynthetic responses of seedlings of the endangered plant Cathaya argyrophylla to different growth light environments[J]. Biodiversity Science, 2005, 13(5): 387-397. [8] 徐祥增, 张金燕, 张广辉, . 光强对三七光合能力及能量分配的影响[J]. 应用生态学报, 2018, 29(1): 193-204. DOI:10.13287/j.1001-9332.201801.008. XU X Z, ZHANG J Y, ZHANG G H, et al. Effects of light intensity on photosynthetic capacity and light energy allocation inPanax notoginseng[J]. Chinese Journal of Applied Ecology, 2018, 29(1): 193-204. [9] 高佳, 崔海岩, 史建国, . 花粒期光照对夏玉米光合特性和叶绿体超微结构的影响[J]. 应用生态学报, 2018, 29(3): 883-890. DOI:10.13287/j.1001-9332.201803.021. GAO J, CUI H Y, SHI J G, et al. Effects of light intensities after anthesis on the photosynthetic characteristics and chloroplast ultrastructure in mesophyll cell of summer maize(Zea mays L.)[J]. Chinese Journal of Applied Ecology, 2018, 29(3): 883-890. [10] 李玉媛. 云南国家重点保护野生植物[M]. 昆明: 云南科技出版社, 2005: 234-235. LI Y Y.Yunnan national key protected wild plants[M]. Kunming: Yunnan Science and Technology Press, 2005: 234-235. [11] 汪松, 解焱. 中国物种红色名录(第一卷红色名录)[M]. 北京: 高等教育出版社, 2004: 326-327. WANG S, XIE Y. China Species Red List(Vol. 1 Red List)[M]. Beijing: Higher Education Press,2004: 326-327. [12] 楚永兴, 欧阳志勤, 张荣贵, . 毛果木莲在云南大围山地区的资源分布现状及保护措施[J]. 林业调查规划, 2011, 36(4): 63-65, 70. DOI:10.3969/j.issn.1671-3168.2011.04.017. CHU Y X, OU Y Z Q, ZHANG R G, et al. Distribution and protection measures of Manglietia hebecarpa resources in Dawei mountain area of Yunnan[J]. Forest Inventory and Planning, 2011, 36(4): 63-65,70. [13] 张畋, 楚永兴. 极度濒危植物毛果木莲的种子检测及育苗技术研究[J]. 林业调查规划, 2013, 38(2):101-106,111. DOI:10.3969/j.issn.1671-3168.2013.02.024. ZHANG T,CHU Y X. Seed detection and seedling nursery technology for endangered plant Trichocarpa manglietia[J]. Forest Inventory and Planning, 2013, 38(2): 101-106,111. [14] 张光飞, 杨波, 苏文华, . 光强对珍稀濒危植物毛果木莲幼苗生长和叶绿素含量的影响[J]. 北方园艺, 2014(17): 80-82. ZHANG G F, YANG B, SU W H, et al. Effect of light intensity on growth and chlorophyll contents of rare and endangered plant Manglietia ventii seedlings[J]. Northern Horticulture, 2014(17): 80-82. [15] 颜超, 王中生, 安树青, . 濒危植物银缕梅(Parrotia subaequalis)不同径级个体的光合能力差异与更新限制[J]. 生态学报, 2008, 28(9): 4153-4161. DOI:10.3321/j.issn:1000-0933.2008.09.012. YAN C, WANG Z S, AN S Q, et al. Differences in photosynthetic capacity among different diameter-classes of Parrotia subaequalis populations and their implications to regeneration limitation[J]. Acta Ecologica Sinica, 2008, 28(9): 4153-4161. [16] 蔡建国, 韦孟琪, 章毅, . 遮阴对绣球光合特性和叶绿素荧光参数的影响[J]. 植物生态学报, 2017, 41(5): 570-576. DOI:10.17521/cjpe.2016.0245. CAI J G, WEI M Q, ZHANG Y, et al. Effects of shading on photosynthetic characteristics and chlorophyll fluorescence parameters in leaves of Hydrangea macrophylla[J]. Chinese Journal of Plant Ecology, 2017, 41(5): 570-576. [17] 李金鹏, 赵和祥, 董然, . 光照强度对两种彩叶玉簪生长及光合特性的影响[J]. 南京林业大学学报(自然科学版), 2012, 36(4): 57-61. DOI:10.3969/j.issn.1000-2006.2012.04.011 LI J P, ZHAO H X, DONG R, et al. The effect of different light intensity on the growth and photosynthesis characteristics of two kinds of colorful Hosta[J]. Journal of Nanjing Forestry University(Natural Sciences Edition), 2012, 36(4): 57-61. [18] 姚志刚, 王中生, 颜超, . 濒危植物银缕梅幼苗对不同光强的光合响应[J]. 南京林业大学学报(自然科学版), 2010, 34(3): 83-88. DOI:10.3969/j.issn.1000-2006.2010.03.017. YAO Z G, WANG Z S, YAN C, et al. The photosynthesis response to different light intensity for the endangered plant Parrotia subaequalis[J]. Journal of Nanjing Forestry University(Natural Sciences Edition), 2010, 34(3): 83-88. [19] WOODWARD F I. Stomatal numbers are sensitive to increases in CO2 from pre-industrial levels[J]. Nature, 1987, 327(6123): 617-618. DOI:10.1038/327617a0. [20] FARQUHAR G D, SHARKEY T D. Stomatal conductance and photosynthesis[J]. Ann Rev Physiol, 1982, 33(1): 317-345. DOI:10.3724/SP.J.1145.2012.00218. [21] 许大全. 光合作用学[M]. 北京: 科学出版社, 2013: 94-95. XU D Q.The Science of Photosynthesis[M]. Beijing: Science Press, 2013: 94-95. [22] ADAMS W W, DEMMIG-ADAMS B. Operation of the xanthophyll cycle in higher plants in response to diurnal changes in incident sunlight[J]. Planta, 1992, 186(3): 390-398. DOI:10.1007/BF00195320. [23] 李晓, 冯伟, 曾晓春. 叶绿素荧光分析技术及应用进展[J]. 西北植物学报, 2006, 26(10): 2186-2196. DOI:10.3321/j.issn:1000-4025.2006.10.037. LI X, FENG W, ZENG X C. Advances in chlorophyll fluorescence analysis and its uses[J]. Acta Botanica Boreali-Occidentalia Sinica,2006, 26(10): 2186-2196. [24] MURCHIE E H, CHEN Y Z, HUBBART S. Interactions between senescence and leaf orient at indetemine in situ pattems of photosynthesis and photoinhibition in field grown rice[J]. Plant Physiol, 1999, 119(2): 553-564. DOI:10.3724/SP.J.1145.2015.02009. [25] BOARDMAN K N. Comparative photosynthesis of sun and shade plants[J]. Annual Review of Plant Physiology, 1977, 28(1): 355-377. DOI:10.1146/annurev.pp.28.060177.002035. [26] 万素梅, 贾志宽, 杨宝平. 苜蓿光合速率日变化及其与环境因子的关系[J]. 草地学报, 2009, 17(1): 27-31. DOI:10.11733/j.issn.1007-0435.2009.01.006. WAN S M, JIA Z K, YANG B P. Relationship between diurnal changes of Alfalfa net photosynthetic rate and environmental factors[J]. Acta Agrectir Sinica, 2009, 17(1): 27-31. [27] 杨万基, 于锡宏, 蒋欣梅, . 弱光胁迫对番茄幼苗光合特性和根系生长的影响[J]. 江苏农业科学, 2017, 45(17): 112-114. DOI:10.15889/j.issn.1002-1302.2017.17.030. YANG W J, YU X H, JIANG X M, et al. Effects of weak light stress on photosynthetic characteristics and root growth of tomato seedlings[J].Jiangsu Agricultural Sciences, 2017, 45(17): 112-114. [28] 武冲, 张晓敏, 尹燕雷, . 遮阴对红叶樱花光合速率和叶绿素荧光参数的影响[J]. 山东农业科学, 2017, 49(2): 48-53. DOI:10.14083/j.issn.1001-4942.2017.02.010. WU C, ZHANG X M, YIN Y L, et al. Effects of shading on net photosynthetic rate and chlorophyll fluorescence parameters in leaves of Prunus serrulata Libdl. ‘Royal Burgundy'[J]. Shandong Agricultural Sciences, 2017, 49(2): 48-53. [29] 敖金成, 苏文华, 张光飞, . 不同光强下对马耳蕨叶绿素荧光参数的日变化[J]. 南京林业大学学报(自然科学版), 2011, 35(1): 135-138. DOI:10.3969/j.issn.1000-2006.2011.01.031. AO J C, SU W H, ZHANG G F, et al. Diurnal changes of chlorophyll fluorescence parameters of Polystichum tsus-simense (Hook)J. Sm. under different light intensities[J]. Journal of Nanjing Forestry University(Natural Sciences Edition), 2011, 35(1): 135-138.

备注/Memo

备注/Memo:
收稿日期:2018-10-09 修回日期:2019-05-06 基金项目:国家水体污染控制与治理科技重大专项项目(2012 ZX 07102-003-04-04)。 第一作者:徐清(2571248649@qq.com),ORCID(0000-0003-1345-571X )。*通信作者:张光飞(gfzhang@ynu.edu.cn),副教授,ORCID(0000-0003-3980-1879 )。
更新日期/Last Update: 2019-11-30