[1]孙利娜,周 群,黄妹平,等.基于SSR标记构建宝巾花品种的分子指纹[J].南京林业大学学报(自然科学版),2019,43(06):060-66.[doi:10.3969/j.issn.1000-2006.201812045]
 SUN Lina,ZHOU Qun,HUANG Meiping,et al.Using the SSR fluorescent labling to establish SSR fingerprint for 131 cultivars in Bougainvillea (Nyctaginaceae)[J].Journal of Nanjing Forestry University(Natural Science Edition),2019,43(06):060-66.[doi:10.3969/j.issn.1000-2006.201812045]
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基于SSR标记构建宝巾花品种的分子指纹
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《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

卷:
43
期数:
2019年06期
页码:
060-66
栏目:
研究论文
出版日期:
2019-11-25

文章信息/Info

Title:
Using the SSR fluorescent labling to establish SSR fingerprint for 131 cultivars in Bougainvillea (Nyctaginaceae)
文章编号:
1000-2006(2019)06-0060-07
作者:
孙利娜12周 群3黄妹平2周长品1徐世松4杨舒婷2杜 铃2甘四明12*唐 庆2*
(1.中国林业科学研究院热带林业研究所, 热带林业研究国家林业局重点实验室, 广东 广州 510520; 2. 广西壮族自治区林业科学研究院, 广西优良用材林资源培育重点实验室, 中南速生材繁育国家林业局重点实验室, 广西 南宁 530002; 3. 厦门市园林植物园, 福建 厦门 361003; 4. 中国热带农业科学院热带作物品种资源研究所, 海南 儋州 571737)
Author(s):
SUN Lina12ZHOU Qun3HUANG Meiping2ZHOU Changpin1XU Shisong4YANG Shuting2DU Ling2GAN Siming12*TANG Qing2*
(1. Key Laboratory of State Forestry Administration on Tropical Forestry Research, Research Institute of Tropical Forestry, Chinese Academy of Forestry, Guangzhou 510520, China;
关键词:
宝巾花 品种分类 SSR标记 指纹图谱 亲缘关系
Keywords:
Bougainvillea cultivar classitication SSR fingerprints genetic relationship
分类号:
S685.99; S722.39
DOI:
10.3969/j.issn.1000-2006.201812045
摘要:
【目的】针对宝巾花(Bougainvillea)品种繁多、同物异名和同名异物多有发生的现象,利用可靠的分子标记技术进行品种的有效分类。【方法】基于15个简单重复序列(SSR)位点,利用荧光标记的引物对131个宝巾花品种进行PCR扩增,扩增产物经毛细管电泳检测,分析品种多样性和遗传距离,并构建品种聚类图和指纹图谱。【结果】15个SSR位点共扩增出85个等位基因,平均每个位点的等位片段数5.60个,Shannon’s 信息指数、观测杂合度和期望杂合度的变化范围分别为0.38~1.53、0.11~0.94和0.16~0.73,平均值分别为1.04、0.50和0.57,位点的多态性信息量介于0.15~0.69之间,平均0.51。宝巾花品种的遗传距离为0.00~0.65,平均0.33; 聚类分析表明,同一种的品种大多数聚在一类,但同一个种仍有品种未聚在一类或亚类,也有多个种的品种聚在一类; 有多对品种存在同物异名或同名异物的现象; 基于11个位点可有效鉴别131个品种,建立了各品种的分子指纹图谱。
Abstract:
【Objective】 Find a reliable molecular marker to identify Bougainvillea cultivars that are difficult to distinguish, due to cultivar richness and cultivar homonymy and synonymy. 【Method】 A total of 15 simple sequence repeats(SSR)markers were used to amplify DNA samples from 131 Bougainvillea cultivars. SSR amplicons were detected by a capillary electrophoresis method. Cultivar polymorphism and genetic distance were calculated; they were used for clustering analysis and fingerprint construction of all the 131 cultivars.【Result】A total of 85 different alleles were collectively generated by the 15 SSR loci, averaging 5.60 alleles per locus. Shannon’s information index, observed heterozygosity value, and expected heterozygosity value ranged from 0.38 to 1.53, 0.11 to 0.94 and 0.16 to 0.73, with an average of 1.04, 0.50 and 0.57 per locus, respectively. The polymorphism information content ranged from 0.15 to 0.69, with an average of 0.51 per locus.

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备注/Memo

备注/Memo:
收稿日期:2018-12-26 修回日期:2019-06-24基金项目:广西科技计划项目(桂科AD17129021); “广西主要用材林资源高效培育与利用人才小高地”专项(桂财社函[2018]112号)。第一作者:孙利娜(sunlina8958170@126.com),工程师。*通信作者:甘四明(Siming_Gan@126.com),研究员,ORCID(0000-0001-6677-9860),负责论文指导与修改; 唐庆(335221247@qq.com),高级工程师,负责论文选题和实验设计指导,ORCID(0000-0003-2351-6889)。
更新日期/Last Update: 2019-11-30