[1]闵莉静,等.基于嗜铁素介导的吡咯伯克霍尔德氏菌JK-SH007促生作用机制研究[J].南京林业大学学报(自然科学版),2019,43(06):164-172.
 MIN Lijing,GUO Lu,et al.Mechanism of Burkholderia pyrrocinia JK-SH007 growth-promoting to plant via siderophore-mediation[J].Journal of Nanjing Forestry University(Natural Science Edition),2019,43(06):164-172.
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基于嗜铁素介导的吡咯伯克霍尔德氏菌JK-SH007促生作用机制研究
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《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

卷:
43
期数:
2019年06期
页码:
164-172
栏目:
研究论文
出版日期:
2019-11-25

文章信息/Info

Title:
Mechanism of Burkholderia pyrrocinia JK-SH007 growth-promoting to plant via siderophore-mediation
文章编号:
1000-2006(2019)06-0165-08
作者:
闵莉静1 2郭 璐3叶建仁1*
(1.南京林业大学林学院,南方现代林业协同创新中心,江苏 南京 210037; 2. 湖州师范学院 生命科学学院,浙江省媒介生物学与病原控制重点实验室,浙江 湖州 313000; 3. 湖州师范学院附属湖州市第一人民医院,浙江 湖州 313000)
Author(s):
MIN Lijing1 2 GUO Lu3 YE Jianren1*
(1.College of Forestry, Nanjing Forestry University, Co-Innovation Center for the Sustainable Forestry in Southern China,Nanjing 210037, China; 2.College of Life Science, Huzhou University, Huzhou 313000, China; 3 Huzhou First People's Hospital, Huzhou University, Huzhou 313000, China)
关键词:
吡咯伯克霍尔德氏菌 嗜铁素 抑菌 促生
Keywords:
Burkholderia pyrrocinia siderophore characterization antifungi activity plant growth promotion
分类号:
S763
摘要:
【目的】分析吡咯伯克霍尔德菌 JK-SH007基于产嗜铁素的促生作用机制。【方法】通过萃取、过柱等操作,分离纯化,获得吡咯伯克霍尔德菌 JK-SH007所产嗜铁素(简称JK-SH007-嗜铁素)的纯品,通过HPLC和LC-MS对其结构进行鉴定,通过与病原菌共培养,测菌丝干质量的方法,分析其对3种杨树溃疡病病原真菌(金黄壳囊孢、拟茎点霉和七叶树壳梭孢)的抑制作用,以商品嗜铁素磺酸去铁胺(DFOB)作为对照,比较JK-SH007-细菌重悬液、1.4 mg/mL JK-SH007-嗜铁素溶液对番茄、黄瓜种子的萌发作用,并继续观察对黄瓜幼苗的促生作用和番茄挂果果品改善效果,评估JK-SH007-嗜铁素的直接与间接的促生作用。【结果】与对照相比,JK-SH007-嗜铁素显示出比DFOB更有效的抑制真菌生长的活性,3种病原真菌抑菌率分别为(89±0.9)%,(76±0.6)%和(90±0.7)%。同时,与JK-SH007-菌悬液相比,JK-SH007-嗜铁素溶液对番茄、黄瓜种子和幼苗活力的提高表现更好,而对于黄瓜幼苗的生长促进作用和番茄挂果的改善作用,其效果与菌悬液相近。【结论】嗜铁素为促生菌吡咯伯克霍尔德氏菌 JK-SH007的主要促生生防因子之一。
Abstract:
【Objective】We aimed to explore the mechanism by which Burkholderia pyrrocinia JK-SH007, a gram-negative bacterium, promotes plant growth; we explored the effects of siderophores on pathogenic fungal activity and plant growth. 【Method】 A sample of JK-SH007-siderophores was isolated and purified using column chromatography, and the siderophore structure was identified using high-performance liquid chromatography and liquid chromatography-mass spectrometry. We cultivated three pathogens that cause poplar canker(Cytospora chrysosperma, Phomopsis macrospora and Fusicoccum aesculi)with siderophore samples to measure pathogen inhibition, and recorded the dry weight of mycelium at the end of the cultivation period. We compared the effects of a JK-SH007-siderophore solution, a JK-SH007 bacterial re-suspension, and a standard siderophore, Deferroamine B solution(DFOB)on plant growth(seed germination, cucumber seedling growth and tomato fruiting).【Result】Compared to DFOB, the JK-SH007-siderophore sample was more effective at inhibiting fungal growth. The pathogenic fungi were inhibited by(89±0.9)%,(76±0.6)% and(90±0.7)%, for C. chrysosperma, P. macrospora and F. aesculin, respectively. Compared to the JK-SH007 re-suspension, the JK-SH007-siderophore solution resulted in better seed germination and higher seedling vigor; however, there were no significant differences for cucumber seedling growth or tomato fruiting. 【Conclusion】We applied a pure siderophore sample, extracted and purified using chromatography, to plants, and compared this with a bacterial re-suspension sample and a standard commercial product. The pure siderophore sample strongly promoted plant growth and the application of a pure siderophore solution can achieve better anti-fungal effects than DFOB, a result which should inform future commercial development of JK-SH007 as a bio-fertilizer.

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相似文献/References:

[1]窦承阳,王 焱,张岳峰,等.3种促生有益微生物在上海梨树上的应用[J].南京林业大学学报(自然科学版),2017,41(04):186.[doi:10.3969/j.issn.1000-2006.201512029]
 DOU Chengyang,WANG Yang,ZHANG Yuefeng,et al.An application of three somatotrophic bacteria on pear in Shanghai[J].Journal of Nanjing Forestry University(Natural Science Edition),2017,41(06):186.[doi:10.3969/j.issn.1000-2006.201512029]
[2]陈飞飞,黄金思,王翕韫,等.吡咯伯克霍尔德氏菌JK-SH007多聚半乳糖醛酸酶基因的克隆及表达分析[J].南京林业大学学报(自然科学版),2018,42(04):127.[doi:10.3969/j.issn.1000-2006.201708013]
 CHEN Feifei,HUANG Jinsi,WANG Xiyun,et al.Cloning and expression analysis of polygalacturonase gene(BpPG) from Burkholderia pyrrocinia JK-SH007[J].Journal of Nanjing Forestry University(Natural Science Edition),2018,42(06):127.[doi:10.3969/j.issn.1000-2006.201708013]

备注/Memo

备注/Memo:
收稿日期:2018-11-28 修回日期:2019-03-27 基金项目:国家自然科学基金项目(31270684); 江苏农业自主创新项目(cx16-1005); 2019年度湖州市第二批科技计划项目(2019Y203); 湖州市自然科学基金项目(2015GY08); 浙江省绿色农药2011协同创新中心开放基金项目(LSNY201820)。 第一作者:闵莉静(178341894@qq.com),博士生。*通信作者:叶建仁(jrye@njfu.edu.cn),教授,ORCID(0000-0001-5711-0516)。
更新日期/Last Update: 2019-11-30