[1]冯 景,沈永宝*,史锋厚.银杏种子脱水敏感性的研究[J].南京林业大学学报(自然科学版),2019,43(06):193-200.
 FENG Jing,SHEN Yongbao*,SHI Fenghou.Study on desiccation sensitivity of Ginkgo biloba seeds[J].Journal of Nanjing Forestry University(Natural Science Edition),2019,43(06):193-200.
点击复制

银杏种子脱水敏感性的研究
分享到:

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

卷:
43
期数:
2019年06期
页码:
193-200
栏目:
研究简报
出版日期:
2019-11-25

文章信息/Info

Title:
Study on desiccation sensitivity of Ginkgo biloba seeds
文章编号:
1000-2006(2019)06-0193-08
作者:
冯 景12沈永宝134*史锋厚134
(1.南京林业大学,南方现代林业协同创新中心,江苏 南京 210037; 2.南京林业大学风景园林学院,江苏 南京 210037; 3.国家林业和草原局南方林木种子检验中心,江苏 南京 210037; 4.南京林业大学林学院,江苏 南京 210037)
Author(s):
FENG Jing12 SHEN Yongbao134* SHI Fenghou134
(1.Co-Innovation Center for the Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China; 2.College of Landscape Architecture, Nanjing Forestry University, Nanjing 210037, China; 3.Southern Tree Seed Inspection Center, National Forestry Administration, Nanjing 210037, China; 4.College of Forestry, Nanjing Forestry University, Nanjing 210037, China)
关键词:
关键词:银杏 脱水敏感性 种子发芽率 顽拗性种子 膜脂过氧化
Keywords:
Ginkgo biloba desiccation sensitivity seed germination percentage recalcitrant seed lipid peroxidation
分类号:
Q945.78
摘要:
【目的】研究银杏种子脱水敏感性及相关的生理生化变化,为银杏种子贮藏、种质资源的保存及苗木生产提供理论依据。【方法】银杏种子于25 ℃、湿度(25±2)%条件下脱水67 d,种子含水量每下降5%左右时随机取样测定相关生理生化指标。【结果】当种子含水量降至45.1%时,发芽率并未有明显变化,然而,当种子含水量降至40.1%时,发芽率从开始的92%下降至50%; 相关性分析表明种子含水量与发芽率之间存在极显著的正相关(r=0.910)。银杏种子脱水过程中,其相对电导率不断上升。此外,SOD活性变化与POD活性的变化基本相似,均呈现出先下降,后升高至最大值,再下降的趋势,只是其POD活性的上升比SOD早,说明POD的反应比SOD更为敏感。种子含水量与硫代巴比妥酸反应产物(TBARS)含量(r=-0.619)、相对电导率(r=-0.745)之间存在极显著的负相关关系。【结论】银杏种子对脱水高度敏感,表现出顽拗型种子特点。严重脱水降低了银杏种子抗氧化酶活性,并加剧了膜脂过氧化作用,导致种子活力下降乃至完全丧失。
Abstract:
【Objective】 The aim of this work was to examine the superoxide dismutase(SOD)and peroxidase(POD)activity and germinability of Ginkgo biloba seeds in response to desiccation, to provide technical guidance for the preservation and storage of its germplasm resources. 【Method】 Seeds were placed in a drying room [(25±2)% relative humidity, 25 ℃)] for 67 days. Samples were randomly taken for germination testing and enzyme activity determination after each ±5% decrease in moisture content. 【Result】The germination percentage remained constant when the seed moisture content decreased from 48.0%(fresh seeds)to 45.1%. However, when the moisture content reached 40.1%, the germination percentage decreased from 92% to 50%. A significant positive correlation was observed between the moisture content and seed germination percentage(r = 0.910). The electrical conductivity was significantly higher during the initial desiccation period(48.0%-45.1%). Furthermore, both SOD and POD activity first reduced, and then increased to peak values before declining again. POD activity rose earlier than SOD activity, indicating that the POD reaction was more desiccation-sensitive than the SOD reaction. Significant negative correlations were observed between the moisture content and thiobarbituric acid-reactive substance content(r =-0.619)and electrical conductivity(r =-0.745). 【Conclusion】Our collective results suggest that G. biloba seeds are highly sensitive to desiccation. Excessive desiccation could reduce the antioxidant enzyme activity of G. biloba seeds and intensify membrane lipid peroxidation, which would cause a consequent reduction—or even a complete loss—of seed germinability.

参考文献/References:

[1] 周志炎.中生代银杏类植物系统发育、分类和演化趋向[J]. 云南植物研究,2003, 25(4): 377-396. DOI:10.3969/j.issn.2095-0845.2003.04.001. ZHOU Z Y. Mesozoic ginkgoaleans: phylogeny, classification and evolutionary trends[J]. Acta Bot Yunnanica, 2003, 25(4): 377-396. [2] MAHADEVAN S, PARK Y.Multifaceted therapeutic benefits of Ginkgo biloba L.: chemistry, efficacy, safety, and uses[J]. Journal of Food Science,2008, 73(1): R14-R19. DOI:10.1111/j.1750-3841.2007.00597.x. [3] 陈雷, 孙冰, 汪贵斌, 等.银杏果用林复合经营模式下种仁品质综合评价 [J]. 林业科学, 2016, 52(11): 63-70. DOI:10.11707/j.1001-7488.20161108. CHEN L, SUN B, WANG G B, et al. A comprehensive evaluation of kernel quality under agroforestry models of Ginkgo biloba plantation for nut production[J]. Scientia Silvae Sinicae, 2016,52(11):63-70. [4] NAKAO Y, KAWASE K, SHIOZAKI S, et al.The growth of pollen and female reproductive organs of ginkgo between pollination and fertilization[J]. Journal of the Japanese Society for Horticultural Science,2001, 70(1): 21-27. DOI:10.2503/jjshs.70.21. [5] JIN B, ZHANG L, LU Y, et al.The mechanism of pollination drop withdrawal in Ginkgo biloba L.[J]. BMC Plant Biology,2012, 12(1): 59. DOI:10.1186/1471-2229-12-59 [6] ZHANG Z, CLAYTON S C, CUI K, et al.Developmental synchronization of male and female gametophytes in Ginkgo biloba and its neck mother cell division prior to fertilization[J]. Physiol Plantarum,2013, 147(4): 541-552. DOI:10.1111/j.1399-3054.2012.01691.x. [7] DAWS M I, GARWOOD N C, PRITCHARD H W.Prediction of desiccation sensitivity in seeds of woody species: a probabilistic model based on two seed traits and 104 species[J]. Annals of Botany,2006, 97(4): 667-674. DOI:10.1093/aob/mcl022. [8] 何惠英, 宋松泉.滇南红厚壳种子的脱水敏感性及其影响萌发的因子[J]. 云南植物研究,2003, 25(6): 687-692. DOI:10.3969/j.issn.2095-0845.2003.06.008. HE H Y, SONG S Q. Desiccation sensitivity of Calophyllum polyanthum seeds and factors affecting their germination[J]. Acta Bota Yunnanica, 2003, 25(6), 687-692. [9] SINGH B, KAUR P, GOPICHAND, et al.Biology and chemistry of Ginkgo biloba[J]. Fitoterapia,2008, 79(6): 401-418. DOI:10.1016/j.fitote.2008.05.007. [10] TOMMASI F, PACIOLLA C, CONCETTA DE PINTO M, et al.Effects of storage temperature on viability, germination and antioxidant metabolism in Ginkgo biloba L. seeds[J]. Plant Physiology and Biochemistry,2006, 44(5): 359-368. DOI:10.1016/j.plaphy.2006.06.014. [11] HONG T D, ELLIS R H. A protocol to determine seed storage behaviour [M]. Rome: International Plant Genetic Resources Institute, 1996. [12] DEL TREDICI P.The phenology of sexual reproduction in Ginkgo biloba: ecological and evolutionary implications[J]. The Botanical Review,2007, 73(4): 267-278. DOI:10.1663/0006-8101(2007)73[267:TPOSRI]2.0.CO; 2. [13] BERJAK P, PAMMENTER N. Orthodox and recalcitrant seeds [C]//VOZZO J A. Tropical tree seed manual agricultural handbook 721. Washington DC: USDA Forest Service, 2002: 137-147. [14] 文彬.试论种子顽拗性的复合数量性状特征[J]. 云南植物研究,2008, 30(1): 76-88. DOI:10.3969/j.issn.2095-0845.2008.01.012. WEN B. On the compound quantitative characteristic trait of seed recalcitrance[J]. Acta Bot Yunnanica 2008,30(1):76-88. [15] 张振玲. 刻叶紫堇种子的休眠与萌发特性研究 [D]. 南京:南京林业大学, 2015. ZHANG Z L. Study on seed dormancy and the germination characteristics of Corydalis incisa [D]. Nanjing: Nanjing Forestry University,2015. [16] 周小梅, 王自霞, 乔燕祥.人工老化处理对芝麻种子生理生化特性的影响[J]. 中国油料作物学报,2008, 30(4): 460-463. DOI:10.3321/j.issn:1007-9084.2008.04.014. ZHOU X M, WANG Z X, QIAO Y X. Effects of artificial aging on physiological and biochemical characteristics of sesame seed[J]. Chinese Journal of Oil Crop Sciences, 2008, 30(4):460-463. [17] BAILLY C, AUDIGIER C, LADONNE F, et al.Changes in oligosaccharide content and antioxidant enzyme activities in developing bean seeds as related to acquisition of drying tolerance and seed quality[J]. Journal of Experimental Botany,2001, 52(357): 701-708. DOI:10.1093/jexbot/52.357.701. [18] 李文君, 沈永宝.‘紫柄籽银桂'桂花种子脱水耐性与抗氧化系统的关系[J]. 园艺学报,2009, 36(2): 279-284. DOI:10.16420/j.issn.0513-353x.2009.02.019. LI W J, SHEN Y B. Changes on physiological characteristics of Osmanthus fragrans ‘Zibing Ziyingui' seeds duringdehydration[J]. Acta Hortic Sin, 2009, 36(2), 279-284. [19] FARRANT J M, PAMMENTER N W, BERJAK P, et al.Subcellular organization and metabolic activity during the development of seeds that attain different levels of desiccation tolerance[J]. Seed Science Research,1997, 7(2): 135-144. DOI:10.1017/S0960258500003470. [20] PUKACKA S, MALEC M, RATAJCZAK E.ROS production and antioxidative system activity in embryonic axes of Quercus robur seeds under different desiccation rate conditions[J]. Acta Physiol Plant,2011, 33(6): 2219-2227. DOI:10.1007/s11738-011-0761-5. [21] 芮海云, 顾龚平.银杏种子的生物学特性及贮藏[J]. 中国野生植物资源,2009, 28(5): 10-13. DOI:10.3969/j.issn.1006-9690.2009.05.003. RUI H Y, GU G P. Study of biological character and storage of gingko seeds[J]. Chinese Wild Plant Resources, 2009, 28(5): 10-13. [22] ISTA. International rules for seed testing [M]. Switzerland: The International Seed Testing Association(ISTA), 2005. [23] XU L, PAN Y, YU F.Effects of water-stress on growth and physiological changes in Pterocarya stenoptera seedlings[J]. Scientia Horticulturae,2015, 190: 11-23. DOI:10.1016/j.scienta.2015.03.041. [24] GIANNOPOLITIS C N, RIES S K.Superoxide dismutases: II. Purification and quantitative relationship with water-soluble protein in seedlings[J]. Plant Physiology,1977, 59(2): 315-318. DOI:10.1104/pp.59.2.315. [25] KOCHBA J, LAVEE S, SPIEGELROY P.Differences in peroxidase activity and isoenzymes in embryogenic and non-embryogenic ‘Shamouti' orange ovular callus lines[J]. Plant & Cell Physiology,1977, 18(2): 463-467. DOI:10.1093/oxfordjournals.pcp.a075455. [26] CAKMAK I, HORST W J.Effect of aluminium on lipid peroxidation, superoxide dismutase, catalase, and peroxidase activities in root tips of soybean(Glycine max)[J]. Physiologia Plantarum,1991, 83(3): 463-468. DOI:10.1111/j.1399-3054.1991.tb00121.x. [27] KING M W, ROBERTS E H. The storage of recalcitrant seeds: achievements and possible approaches [M]. Rome: International Board for Plant Genetic Resources, 1979. [28] YU F Y, DU Y, SHEN Y B.Physiological characteristics changes of Aesculus chinensis seeds during natural dehydration[J]. Journal of Forestry Research,2006, 17(2): 103-106.DOI:10.1007/s11676-006-0024-2. [29] FAROOQ M, WAHID A, KOBAYASHI N, et al. Plant drought stress: effects, mechanisms and management [M]// Sustainable Agriculture. Netherlands: Springer, 2009: 153-188. DOI:10.1007/978-90-481-2666-8_12. [30] VERTUCCI C, FARRANT J, CRANE J.The status of and requirement for water in developing bean seeds[M]// CLOSE T J, BRAY E A. Plant response to cellular dehydration during environmental stress. Rockville: American Society of Plant Physiologists, 1993: 259-260. [31] DRESCH D M, MASETTO T E, SCALON S P Q.Campomanesia adamantium(Cambess.)O. Berg seed desiccation: influence on vigor and nucleic acids[J]. Anais da Academia Brasileira de Ciências,2015, 87: 2217-2228. DOI:10.1590/0001-3765201520140539. [32] BERJAK P, PAMMENTER N W.Implications of the lack of desiccation tolerance in recalcitrant seeds[J]. Frontiers in Plant Science,2013, 4: 478. DOI:10.3389/fpls.2013.00478. [33] VELJOVIC-JOVANOVIC S, KUKAVICA B, STEVANOVIC B, et al.Senescence-and drought-related changes in peroxidase and superoxide dismutase isoforms in leaves of Ramonda serbica[J]. Journal of Experimental Botany,2006, 57(8): 1759-1768. DOI:10.1093/jxb/erl007. [34] VELJOVIC-JOVANOVIC S, KUKAVICA B, NAVARI-IZZO F.Characterization of polyphenol oxidase changes induced by desiccation of Ramonda serbica leaves[J]. Physiol Plant,2008, 132(4): 407-416. DOI:10.1111/j.1399-3054.2007.01040.x. [35] JAMALOMIDI M, GHOLAMI M.Effect of desiccation on antioxidant enzymes activity of recalcitrant tea(Camellia sinensis L.)seeds[J]. Int Res J Appl Basic Sci,2013, 4(12): 4318-4322. [36] WANG Y, LI S, HE J, et al.Changes in activity of reactive-oxygen-scavenging enzymes in recalcitrant wampee(Clausena lansium)seeds duing desiccation[J]. Acta Phytophysiologica Sinica,2000, 27(1): 81-86. [37] 蒋明义, 荆家海, 王韶唐.渗透胁迫对水稻幼苗膜脂过氧化及体内保护系统的影响[J]. 植物生理学报,1991, 17(1): 80-84. DOI:10.3321/j.issn:1671-3877.1991.01.014. JIANG M Y, JIN J H, WANG S T. Effects of osmotic stress on membrane-lipid peroxidation and endogenous protective systems in ricse seedlings[J].Acta Phytophysiologica Sinica, 1991, 17(1): 80-84. [38] GREGGAINS V, FINCH-SAVAGE W E, ATHERTON N M, et al.Viability loss and free radical processes during desiccation of recalcitrant Avicennia marina seeds[J]. Seed Science Research,2001, 11(3): 235-242. DOI:10.1079/SSR200179. [39] MCDONALD M.Seed deterioration: physiology, repair and assessment[J]. Seed Science and Technology,1999, 27(1): 177-237. [40] CHEN Q, YANG L, AHMAD P, et al.Proteomic profiling and redox status alteration of recalcitrant tea(Camellia sinensis)seed in response to desiccation[J]. Planta,2011, 233(3): 583-592. DOI:10.1007/s00425-010-1322-7. [41] 宋松泉, 傅家瑞.黄皮种子脱水敏感性与脂质过氧化作用[J]. 植物生理学报,1997, 23(2): 163-168. DOI:10.3321/j.issn:1671-3877.1997.02.010. SONG S Q, FU J R. Desiccation-sensitivity and lipid peroxidation in Chinese wampee[Clausena lansium(Lour.)Skeels] seeds[J]. Acta Phytophysiologica Sinica, 1997, 23(2):163-168. [42] XIN X, JING X M, LIU Y, et al.Viability loss pattern under rapid dehydration of Antiaris toxicaria axes and its relation to oxidative damage[J]. Journal of Integrative Plant Biology,2010, 52(5): 434-441. DOI:10.1111/j.1744-7909.2010.00924.x. [43] NTULI T M, FINCH-SAVAGE W E, BERJAK P, et al.Increased drying rate lowers the critical water content for survival in embryonic axes of English oak(Quercus robur L.)seeds[J]. J Integr Plant Biol,2011, 53(4): 270-280. DOI:10.1111/j.1744-7909.2010.01016.x. [44] SONG S Q, BERJAK P, PAMMENTER N.Desiccation sensitivity of Trichilia dregeana axes and antioxidant role of ascorbic acid[J]. Acta Botanica Sinica,2004, 46(7): 803-810. [45] VARGHESE B, NAITHANI S.Desiccation-induced changes in lipid peroxidation, superoxide level and antioxidant enzymes activity in neem(Azadirachta indica A. Juss)seeds[J]. Acta Physiol Plant,2002, 24(1): 79-87. DOI:10.1007/s11738-002-0025-5. [46] 李永红, 马颖敏.不同脱水方式对马拉巴栗种子脱水敏感性和膜脂过氧化的影响[J]. 热带作物学报,2008, 29(6): 738-743. DOI:10.3969/j.issn.1000-2561.2008.06.012. LI Y HONG, MA Y M. Effects of drying at different rates on desiccation sensitivity and membrane lipid peroxidation of Pachira macrocarpa seeds[J]. Chinese Journal of Tropical Crops, 2008, 29(6): 738-743.

备注/Memo

备注/Memo:
收稿日期:2018-08-13 修回日期:2019-08-02 基金项目:江苏高校优势学科建设工程资助项目(PAPD)。 第一作者:冯景(fengjing9520@yeah.net),博士。*通信作者:沈永宝(ybshen@njfu.edu.cn),教授,ORCID(0000-0002-0440-5086)。
更新日期/Last Update: 2019-11-30