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Genetic variation of fiber traits in Populus deltoides clones
刘玉鑫1颜开义2何 伟1潘惠新1*
(1.南京林业大学林学院,江苏 南京 210037; 2.泗洪县林业科技推广中心,江苏 宿迁 223900)
LIU Yuxin1 YAN Kaiyi2 HE Wei1 PAN Huixin1*
(1. College of Forestry,Nanjing Forestry University,Nanjing 210037,China; 2. Sihong Forestry Center, Jiangsu Province, Suqian 223900, China)
美洲黑杨 木材纤维 纤维长度 纤维宽度 遗传变异 无性系
Populus deltoides wood fiber fiber length fiber width genetic variation clones
【Objective】Populus deltoides is one of the largest timber species planted in the orthern Jiangsu and it has important economic and ecological value. In recent years, with the increase in market demand for poplar wood and higher requirements for wood quality, the research on poplar wood property improvement is necessary. Among the wood properties, fiber length and ratio of fiber length to width directly affect the pulping performance and paper quality of poplar. In this study, we aimed to investigate the general patterns of genetic variation in fiber traits of P. deltoides and to provide the scientific basis for poplar wood improvement.【Method】Eleven ten-year-old P. deltoides clones were used as materials. Sample trees of each clone were selected in each of the four blocks. After measuring the tree height and diameter at breast height(DBH), sample trees were cut down and the wooden discs were intercepted at 1.3 m from the ground and brought back to the laboratory for air-drying. After the samples were treated with the delignification reagent, the fiber traits were measured by optical microscope and MANUEL MORFI. The SPSS 19.0 software was used to analyze and process the data, and the genetic variation and correlation of each trait were analyzed. The K-means method was used to cluster the 11 clones, and among them, the clones with excellent fiber and growth characters were selected.【Result】The regression analysis of values measured by the two methods showed that R2 were more than 0.9, thus the MORFI measurement values were reliable. The fiber length and fiber length to width ratio increased with the increase of forest age. The average values of fiber length, fiber width, and fiber length to width ratio of 11 clones were 1 107.8 μm, 22.3 μm and 49.57, respectively. There were significant differences in fiber traits among different clones. The heritability of the three fiber traits was 0.764, 0.832 and 0.590, respectively. The correlation coefficients between fiber length and growth character were between 0.21 and 0.25, and those between fiber width and growth character were between 0.39 and 0.48. The correlation coefficients between fiber length to width ratio and growth character were between -0.07 and -0.02. Eleven clones could be classified into three categories.【Conclusion】The MANUEL MORFI is able to quickly and accurately measure fiber length and width, which was suitable for the measurement of mass data, but the results had to be adjusted by regression. The values of fiber length and ratio of fiber length to width increased with the increase of forest age, which implies that the pulping performance of wood was better with the increase of forest age. Correlation coefficients between different ages were high. These results provided a possibility for early selection of fiber traits in P. deltoides. Genetic variations in fiber traits were observed in different clones, and the heritability of each trait ranged from 0.366 to 0.832. The independent selection method could be used for genetic improvement of P. deltoides. Among all 11 clones, 2-2, 4-6, 4-45 and 4-50 had better fiber and growth properties than those of the rest, and these clones should be further tested to create conditions for new breeding


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收稿日期:2019-04-25 修回日期:2019-07-02基金项目:江苏省科技项目现代农业项目(BE2016387)。 第一作者:刘玉鑫(1060695487@qq.com)。*通信作者:潘惠新(hxpan@njfu.com.cn),教授,ORCID(0000-0002-0756-4680)。
更新日期/Last Update: 2019-03-25