水曲柳胚后熟时期MSAP分析

丛建民; 陈凤清; 沈海龙; 李玉花; 张鹏; 杨玲

doi:10.3969/j.issn.1000-2006.2015.03.008

水曲柳胚后熟时期MSAP分析

丛建民,陈凤清,沈海龙2,4*,李玉花3,4,张鹏2,4,杨玲2,4

南京林业大学学报（自然科学版） ›› 2015, Vol. 39 ›› Issue (03) : 39-44.

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南京林业大学学报（自然科学版） ›› 2015, Vol. 39 ›› Issue (03) : 39-44. DOI: 10.3969/j.issn.1000-2006.2015.03.008

研究论文

水曲柳胚后熟时期MSAP分析

丛建民¹,陈凤清¹,沈海龙^2,4*,李玉花^3,4, 张鹏^2,4,杨玲^2,4

作者信息 +

Research on the features of DNA methylation in embryo ripening period ofFraxinus mandshurica Rupr. based on the method of MSAP

CONG Jianmin¹,CHEN Fengqing¹,SHEN Hailong^2,4*,LI Yuhua^3,4, ZHANG Peng^2,4,YANG Ling^2,4

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摘要

为研究水曲柳合子胚发育后熟时期的基因组表观调控模式,以7月上旬到9月中旬8个取材时期多年生水曲柳合子胚子叶为研究对象,通过基因组甲基化敏感扩增多态性分析,检测了各时期基因组甲基化变化。结果表明:水曲柳合子胚在不同发育时期可检测到933～939条清晰的电泳条带,说明不同发育时期水曲柳合子胚甲基化差异不明显。进一步统计胞嘧啶甲基化模式及其变异模式发现,7月初基因组在CNG、CG/CNG位点下降的变异位点多于其他时期,表明在水曲柳合子胚发育过程中发生了较大规模的去甲基化事件。

Abstract

To research epigenetic regulation of genome of Fraxinus mandshurica seed development ripening period, Fraxinus mandshurica cotyledons from early July to mid-September 8 stages were selected as test material. Through MSAP(methylation-sensitive amplified polymorphism)method, we detected the change of DNA ethylation in each period. The result indicated that no significant difference among different development stages was detected(in total 933-939 clearly identified strips was detected in each stage). However, the cytosine methylation patterns and variation pattern of CCGG sites showed that, in early July, CNG, CG/CNG loci demethylation sites were higher than other stage, which indicated that large scale of DNA demethylation occurred following seed development of Fraxinus mandshurica. This result could serve as the regulation patterns and as an important basis for providing theoretical basis for seed development.

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丛建民,陈凤清,沈海龙2,4*,李玉花3,4,张鹏2,4,杨玲2,4. 水曲柳胚后熟时期MSAP分析[J]. 南京林业大学学报（自然科学版）. 2015, 39(03): 39-44 https://doi.org/10.3969/j.issn.1000-2006.2015.03.008

CONG Jianmin,CHEN Fengqing,SHEN Hailong^2,4*,LI Yuhua^3,4, ZHANG Peng^2,4,YANG Ling^2,4. Research on the features of DNA methylation in embryo ripening period ofFraxinus mandshurica Rupr. based on the method of MSAP[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2015, 39(03): 39-44 https://doi.org/10.3969/j.issn.1000-2006.2015.03.008

中图分类号： Q78 S718

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基金

收稿日期:2014-09-12 修回日期:2015-01-08
基金项目:国家自然科学基金项目(30872008)
第一作者:丛建民,副教授,博士。*通信作者:沈海龙,教授。E-mail: shenhl-cf@ nefu.edu.cn。
引文格式:丛建民,陈凤清,沈海龙,等. 水曲柳胚后熟时期MSAP分析[J]. 南京林业大学学报:自然科学版,2015,39(3):39-44.