<正>从复合材料力学理论出发，建立了不对称人造板耦合刚度矩阵计算模型，讨论了耦合刚度系数B11、B22与板坯翘曲度的关系，分析了板坯层数、铺层顺序和铺设方向等对板坯耦合效应的影响。结果表明：板坯耦合刚度矩阵B中各元素绝对值越大，其翘曲度越大；在B矩阵中，系数B11和B22值对板坯翘曲度影响较大。可以通过改变板的层数、铺层顺序及铺设方向等因素来进行不对称人造板的结构设计，减少板坯的翘曲度。

Using mature zygotic embryos and immature zygotic embryos as initial explants, the effect of the following factors on induction callus and differentiation adventitious buds.. basal medium, plant hormone and development stages of zygotic embryo were studied. Results indicated that the optimal medium for mature zygotic embryos induction callus was 1/2MS+2mg/L 2,4-D+1.0mg/L 6-BA+1. 0mg/L KT+30 g/L sucrose. This medium can induce soft, transparent callus from immature zygotic embryos of embryo selection or tissue and organ differentiation stages. The induction frequency was 2%～6%. Immature zygotic embryos of tissue and organ differentiation or embryo mature stages induction callus were similar in state with those of mature zygotic embryo. The callus induction frequency was significantly improved with the maturity of immature zygotic embryos. The optimal medium for differentiation adventitious buds was DCR+1.5mg/L 6-BA+1. 5mg/L KT+20 g/L sucrose. Shoots with 1. 5 cm high were transfered to rooting medium and rooting frequency reached 100%.