南京林业大学学报(自然科学版) ›› 2003, Vol. 27 ›› Issue (03): 55-58.doi: 10.3969/j.jssn.1000-2006.2003.03.013

• 研究论文 • 上一篇    下一篇

杉木单染色体扩增产物的RAPD分析

李湘阳;周坚;袁汉升;张再良   

  1. 南京林业大学;江苏 南京 210037;南京林业大学;江苏 南京 210037;湖南永州市林业局;湖南 永州 425000;湖南永州市林业局;湖南 永州 425000
  • 出版日期:2003-06-18 发布日期:2003-06-18

RAPD Studies on the Amplified Product of Single Microdissected Cunninghamia lanceolata Chromosome

LI Xiangyang1,ZHOU Jian1*,YUAN Hansheng2,ZHANG Zailiang2   

  1. 1.Nanjing Forestry University,Nanjing 210037,China;2.Forestry Bureau of Yongzhou Hunan Province,Yongzhou 425000,China
  • Online:2003-06-18 Published:2003-06-18

摘要: <正>在显微操作器上微分离杉木(Cunninghamialanceolata(Lamb.)Hook)的4条单染色体,以简并寡核苷酸为引物进行PCR扩增(DOP PCR),并将其DOP PCR产物进行RAPD分析。结果表明,不同染色体扩增产物间有明显多态性;用RAPD PCR的方式可反应出不同染色体遗传信息的差异,从这些差异中可能找到属于某单染色体的特异信息,进而在分子水平上对染色体进行编号。

Abstract: Four single chromosome of Cunninghamia lanceolata was dissected under the micromanipulator.After degenerate oligonucleotideprimedPCR amplification(DOPPCR),DNA product were tested with RAPD.The results indicated: the polymorphism among different single microdissected Cunninghamia lanceolata chromosome was evident.This research provides a new method to identify microdissected Cunninghamia lanceolata chromosome and locate gene to chromosome of Cunninghamia lanceolata chromsome.

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