赤枝叶片的离体培养与植株再生

关亚丽,黄敏仁,王明麻

南京林业大学学报(自然科学版) ›› 2005, Vol. 29 ›› Issue (03) : 45-48.

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南京林业大学学报(自然科学版) ›› 2005, Vol. 29 ›› Issue (03) : 45-48. DOI: 10.3969/j.jssn.1000-2006.2005.03.011
研究论文

赤枝叶片的离体培养与植株再生

  • 关亚丽,黄敏仁,王明麻
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In Vitro Plant Regeneration from the Seedling Leaves of Eucalyptus camaldulensis

  • GUAN Ya-li, HUANG Min-ren, WANG Ming-xiu
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摘要

<正>利用遥感数据结合野外调查结果,以混合分类法对从洪泽湖天然湿地2002年8月21日获取的ETM+数据进行分类,并生成2002年分类结果在红光、近红外波段特征空间上的分布图。同时。对1979年的LandsatMSS进行聚类分析,并将聚类结果输出到红光(MSS5)、近红外(MSS7)特征空间图像上,根据MSS的假彩色合成影像、野外调查访问结果和聚类结果在红光、近红外波段特征空间上位置间的相互关系对聚类结果进行标识,将两期遥感图像的分类结果进行叠加,比较了1979~2002年洪泽湖天然湿地变化情况。结果显示,1979~2002年洪泽湖湿地丧失了6458.59hm。,这种丧失与当地对湖泊湿地过度开发及不合理利用密切相关。

Abstract

In vitro seedling leaves were used as explants to study the shoot inducing and plant regeneration of E. camaldulensis. The inducing rate of the adventitious shoot was 35. 4% when the explants were cultured on medium of MS+0. 8 mg/L BA+0. 3 mg/L KT+0.05 mg/L NAA,but the inducing rate could be stimulated up to 42.9% when medium of MS+0. 8 mg/L BA+0.05 mg/L TDZ+0. 1 mg/L NAA was used. A ten-day darkness culture could improve the adventitious shoot inducing and the explants from more tender leaves were easier to induce the shoots. The shoots with 2~3 cm length were cultured on the rooting medium and the rooting rate of 100% could.be obtained 30 days later. The transplanting rates of rooted shoots were over 80%.

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关亚丽,黄敏仁,王明麻. 赤枝叶片的离体培养与植株再生[J]. 南京林业大学学报(自然科学版). 2005, 29(03): 45-48 https://doi.org/10.3969/j.jssn.1000-2006.2005.03.011
GUAN Ya-li, HUANG Min-ren, WANG Ming-xiu. In Vitro Plant Regeneration from the Seedling Leaves of Eucalyptus camaldulensis[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2005, 29(03): 45-48 https://doi.org/10.3969/j.jssn.1000-2006.2005.03.011
中图分类号: S723   

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