<正>采用凝胶电泳技术（SDS-PAGE）对1年生杨树无性系797杨春季芽萌发阶段皮层、木质部和根系中的蛋白质含量及营养贮藏蛋白质组分进行了测定分析。结果表明：杨树体内（木质部、皮层、根部）蛋白质含量在芽萌发期间处于最低水平；木质部和皮层是杨树越冬期间贮藏蛋白质的主要场所；杨树新梢萌发期间贮藏蛋白质组分变化最明显的是36、32、18、16kD等蛋白，其中36、32kD蛋白在萌发前均可以清晰地辨认，叶子展开4周以后逐渐消失，而18、16kD两种蛋白仅在发叶期间出现，之后完全消失，36、32kD蛋白是杨树营养贮藏蛋白质的主要类型。

Negative air ion is good to people’s health and is an important resource in tourism region. Status o[ negative air ion in forest environment has become the issue of the day in re cent years. This paper reviewed the negative air ion formation mechanism, its functions on organisms and environment, and the contents, influence factors and spatio--temporal change characteristics of negative air ions in forest environment. Highlights were given to measuring devices, research method, and evaluation criteria, daily and seasonal change of negative air ion, and effects of forest stand, weather condition and water body on forest negative air nion. The main prohlems in the past work and the direction for the future research were also discussed.