与美洲黑杨抗黑斑病基因连锁的SCAR标记的开发

张燕梅,潘惠新,张博,王明庥,黄敏仁*

南京林业大学学报(自然科学版) ›› 2007, Vol. 31 ›› Issue (01) : 15-18.

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南京林业大学学报(自然科学版) ›› 2007, Vol. 31 ›› Issue (01) : 15-18. DOI: 10.3969/j.jssn.1000-2006.2007.01.004
研究论文

与美洲黑杨抗黑斑病基因连锁的SCAR标记的开发

  • 张燕梅,潘惠新,张博,王明庥,黄敏仁*
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Development of SCAR Markers Linked to Resistance Locus of Black Spot Disease in Populus deltoides

  • ZHANG Yan-mei, PAN Hui-xin, ZHANG Bo, WANG Ming-xiu, HUANG Min-ren*
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摘要

<正>以1年生金丝小枣嫁接苗为试材,研究硅对盆栽苗在盐胁迫下各胱甘肽过氧化物酶(GSH-PX)、超氧化物歧化酶(SOD)和过氧化物酶(POD)活性的影响。结果表明:在盐浓度(NaCl质量分数)小于0.4%时,SOD和GSH-PX的活性随着土壤含盐量的增加而增加。POD活性在NaCl质量分数为0.25%的盐胁迫下升高,在NaCI质量分数为0.4%的盐胁迫时下降;盐胁迫下加硅可以显著提高金丝小枣SOD、POD和GSH-Px的活性且差异显著。笔者认为盐胁迫下给土壤施硅可以缓解盐分对金丝小枣的毒害作用。

Abstract

RAPD markers OPAI17-1550 and OPAI13-900, linked to anti-black-spot disease gene locus, were successfully converted into SCAR markers named SAI17-1570 and SAI13-292 respectively by sequencing and designing of primers. SAI17-1570 and SAI13-292 were validated in the resistant、 susceptible pools and F1 population. The SAI17-1570 had the same amplification with templates of the positive OPAI17-1550 F1, population and resistant pool, did not have amplification with templates of the negative OPAI17-1550 F1 population and susceptible pool. The SAI13-292 had one band with templates of the negative OPAI13-900 F1 population and susceptible pool, but two bands with templates of the positive OPAI13-900 F1 population and resistant pool. The two SCAR markers could be of great importance for clone selection on seedling stage.

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张燕梅,潘惠新,张博,王明庥,黄敏仁*. 与美洲黑杨抗黑斑病基因连锁的SCAR标记的开发[J]. 南京林业大学学报(自然科学版). 2007, 31(01): 15-18 https://doi.org/10.3969/j.jssn.1000-2006.2007.01.004
ZHANG Yan-mei, PAN Hui-xin, ZHANG Bo, WANG Ming-xiu, HUANG Min-ren*. Development of SCAR Markers Linked to Resistance Locus of Black Spot Disease in Populus deltoides[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2007, 31(01): 15-18 https://doi.org/10.3969/j.jssn.1000-2006.2007.01.004
中图分类号: S722    Q813   

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