Abstract: RAPD markers OPAI17-1550 and OPAI13-900, linked to anti-black-spot disease gene locus, were successfully converted into SCAR markers named SAI17-1570 and SAI13-292 respectively by sequencing and designing of primers. SAI17-1570 and SAI13-292 were validated in the resistant、 susceptible pools and F1 population. The SAI17-1570 had the same amplification with templates of the positive OPAI17-1550 F1, population and resistant pool, did not have amplification with templates of the negative OPAI17-1550 F1 population and susceptible pool. The SAI13-292 had one band with templates of the negative OPAI13-900 F1 population and susceptible pool, but two bands with templates of the positive OPAI13-900 F1 population and resistant pool. The two SCAR markers could be of great importance for clone selection on seedling stage.