Abstract: In the wood-based panel industry, hot-pressing is one of the most important processes which are highly related to the panel quality. Local internal-mat conditions of temperature, moisture content, stress history, and gas pressure influence wood densification, adhesive cure, and the subsequent panel properties. A measuring system for wood-based panel hot-pressing which was designed and developed based on virtual instrument and LabVIEW graphic program language were introduced. This system provides a flexible and powerful tool for real-time measuring the internal-mat environment during wood-based panel hot-pressing, and can be very useful in parameter setting and optimizing.