南京林业大学学报(自然科学版) ›› 2007, Vol. 31 ›› Issue (04): 1-5.doi: 10.3969/j.jssn.1000-2006.2007.04.001

• 研究论文 •    下一篇

松萎蔫病病原的研究

赵博光1,赵林果2,刘秀华1,莫广涛1,郭建1,商庆清3   

  1. 1. 南京林业大学森林资源与环境学院, 江苏 南京 210037; 2. 南京林业大学化学工程学院, 江苏 南京 210037; 3. 南京林业大学机械电子工程学院, 江苏 南京 210037
  • 出版日期:2007-08-18 发布日期:2007-08-18

Study on the Pathogens of Pine Wilt Disease

ZHAO Bo-guang1, ZHAO Lin-guo2, LIU Xiu-hua1, MO Guang-tao1, GUO Jian1, SHANG Qing-qing3   

  1. 1. College of Forest Resources and Environment Nanjing Forestry University, Nanjing 210037, China; 2. College of Chemical Engineering Nanjing Forestry University, Nanjing 210037, China; 3. College of Electronic and Mechanical Engineering Nanjing Forestry University, Nanjing 210037, China
  • Online:2007-08-18 Published:2007-08-18

摘要: <正>采用组织分离法和平板系列稀释法,从健康和感染松枯梢病的松树针叶上分离叶栖细菌891株。通过室内平板对峙培养,初步筛选出16株对枯梢病菌丝有强抑制作用的拮抗细菌,抑菌圈直径均大于20mm。在此基础上选取具有代表性的10个菌株进行温室马尾松盆栽苗防病试验,从中筛选出3株(菌株编号为N29、S(1)102、M(2)21)抑菌圈直径分别为28.5、37.5、31.0mm及防效达60%以上并对松苗无不良影响的拮抗细菌。

Abstract: Pine wilt disease has become a worldwide threat to pine forests and forest ecosystem. However, its mechanism has not been clearly illustrated, though most scientists working in the area believe that pine wood nematode (PWN) is the only pathogen of the disease. This study indicated the bacteria symbiotically associated with the nematode played an important part in the disease. A series of inoculation experiments were completed with a new developed experimental device, cold valve, by which the nematode and its associated bacteria were confined within the inoculated branch. Here this paper show that the pine wilt disease is a complex disease caused by a complex of PWN and its associated pathogenic bacteria. The results also indicated that without the movement of the two pathogens to other parts of the inoculated pine the axenic PWN the systemic toxins in the culture produced by the pathogenic bacterial strain resulted in wilt and death of the host. Based on the results of the study and reports in the mixed with the pathogenic bacterium, Pseudomonas fluorescens (GcM5-1A) or the cell free culture of the bacterium were able to cause the disease. The bacterium was reisolated from the dead host, inoculated with axenic PWN and the bacterial strain proving Koch’s postulates. The result’ from the inoculation of the cell free culture of the bacterial strain in indicated that it was the systemic toxins in the culture produced by the pathogenic bacterial strain that resulted in wilt and death of the host. Based on results of the study and previous reports of the authors on mutualistic symbiosis between PWN and its associated bacteria, the authors pointed out that pine wilt disease is the first experimentally confirmed plant disease induced by a mutualistic symbiotic nematode-bacterial complex.

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