南京林业大学学报(自然科学版) ›› 2007, Vol. 31 ›› Issue (04): 121-124.doi: 10.3969/j.jssn.1000-2006.2007.04.027

• 研究论文 • 上一篇    下一篇

松材线虫实时PCR检测技术

陈凤毛1,2,叶建仁1*,吴小芹1   

  1. 1. 南京林业大学森林资源与环境学院, 江苏 南京 210037; 2. 安徽省林业有害生物防治检疫局, 安徽 合肥 230031
  • 出版日期:2007-08-18 发布日期:2007-08-18

Detection Technique of Bursaphelenchus xylophilus Using Real Time PCR

CHEN Feng-mao1,2, YE Jian-ren1*, WU Xiao-qin1   

  1. 1. College of Forest Resources and Environment Nanjing Forestry University, Nanjing 210037, China; 2. Anhui General Station of Forest Pest Management, Hefei 230031, China
  • Online:2007-08-18 Published:2007-08-18

摘要: <正>城市森林群落结构是城市森林研究的基础,是由不同植物种类及其在空间分布的不同格局决定的。笔者从群落的多样性、群落的动态、群落树种的选择及人工群落配置模式3个方面论述了国内外对城市森林植物群落结构的研究现状,分析了城市森林群落结构研究的发展趋势。

Abstract: In this paper, a set of primers (F11/R11) and probe (TaqMan-11) specific for Bursaphelenchus xylophilus was designed to target the ribosomal DNA internal transcribed spacer (ITS) region, which composed the real-time polymerase chain reaction (PCR) assay. Then, B. xylophilus, B. mucronatus, B. hofmanni, A. macronucleatus and Seinura sp. separated from dead pine were detected a using this real-time PCR assay. The results showed that fluorescent signals were detected for all B. xylophilus isolates and there was no signal for B. mucronatus, B. hofmanni, Aphelenchoides macronucleatus, Seinura sp. and water (control). This indicated that the probe (TaqMan-11) and primers (F11/R11) were highly specific for B. xylophulus. Besides, the assay was very sensitive, detecting as little as 0.005 pg of B. xylophilus DNA in 10μL volume. The real-time PCR assay also successfully detected single pinewood nematodes, and it should be very useful for quarantine purposes.

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