<正>利用不同强度磁场处理马尾松种子，研究马尾松种子活力及其POD、SOD活性的变化。结果发现：中等活力的马尾松种子经0.2T或0.3T磁场处理10min或15min，发芽率明显提高，尤以0.3T处理15min最为明显，发芽率提高到83%。这些处理条件对高活力马尾松种子的发芽率没有显著影响，但经0.3T磁场处理15min后种子的发芽势由32%提高到46%。此外，随着处理时间的延长，中等活力和高活力种子过氧化物酶活性均逐渐增强。中等活力种子经0.3T磁场处理15min，过氧化物酶活性比对照几乎增加了一倍，高活力种子过氧化物酶活性比对照增加了74%。磁场处理使中等活力马尾松种子超氧化物歧化酶活性由0.20（U/（g?min））提高到0.79U/（g?min），却降低了高活力种子超氧化物歧化酶的活性。

The purification steps, e.g. a two-step ultrafiltration, cationic (CM-Sepharose FF) and anionic (DEAE-Sepharose FF) chromatography and size exclusion (Sephadex G-100) chromatography and the six homogeneous cellulase etc, were used to purify three endoglucanases (EG Ⅰ, EG Ⅱ and EG Ⅲ), two cellobiohydrolases (CBH Ⅰ and CBH Ⅱ) and a (β-glucosidase (GB) according to SDS—PAGE analysis from the culture broth of Trichoderma reesei. The specific activities of EG Ⅰ, EG Ⅱ and EG Ⅲ with carboxymethyl celluloses were estimated to be 176.35, 153.96, 64.22IU/mg, respectively. For CBH I and CBH Ⅱ, their specific activities with microcelluloses were 16.86, 4.82IU/mg, respectively. The GB with salicin was 31.00IU/mg. Their Km were 6.70, 8.46, 13.22, 1.37, 3.46, 2.20mg/mL, respectively. The greater Km of the purified enzyme component of the same class is, the smaller their turnover is. Their molecular weights of EG Ⅰ, EG Ⅱ, EG Ⅲ, CBH I, CBH Ⅱ and GB were 50, 46, 25, 65, 58, 75kDa, respectively.