抗、感病杨树与溃疡病菌互作中活性氧及相关酶的动态

王媛1,2,梁军1,张星耀1*

南京林业大学学报(自然科学版) ›› 2008, Vol. 32 ›› Issue (05) : 41-46.

南京林业大学学报(自然科学版) ›› 2008, Vol. 32 ›› Issue (05) : 41-46. DOI: 10.3969/j.jssn.1000-2006.2008.05.008
研究论文

抗、感病杨树与溃疡病菌互作中活性氧及相关酶的动态

  • 王媛1,2,梁军1,张星耀1*
作者信息 +

Changes of active oxygen and related enzymes during the interaction of poplar and canker disease pathogen

  • WANG Yua1,2, LIANG Jan1, ZHANG Xin-yao1*
Author information +
文章历史 +

摘要

<正>采用随机区组试验设计方法,开展了基质配比和容器规格对武当木兰Magnolia sprengeri容器苗生长影响的研究。结果表明:不同基质配比和容器规格对苗木质量均有显著影响。以腐殖质为主的基质配比容器苗生长较好,而以黄绵土或沙土为主的基质配比容器苗生长较差;武当木兰幼苗根系发达,从生产实际出发,容器规格以10 cm×20 cm最佳。提高武当木兰容器育苗质量的最佳培养方案为:50%腐殖质+50%细河沙,选用10 cm ×20 cm的容器,该组合既能保证容器苗的苗高、地径、单株生物量等各项指标充分生长,又能节约育苗成本,同时根系与基质能形成紧密的根系团。

Abstract

The dynamic changes of superoxide anion O2-, hydrogen peroxide (H202), superoxide dismutase (SOD), eatalase (CAT), peroxidase (POD), ascorbate peroxidase (APX) and malondialdehyde (MDA) in the barks of resistant and susceptible poplar cultivars, Populus tomentosa and P. beijingensis infected with Botryosphaeria dothidea, were measured, and the generation of reactive oxygen species (ROS) in the interaction between poplar cuhivars and Botryosphaeria pathogen, the activity change rule of oxidation enzyme-resistance and their relationship with the disease resistance of testing poplars were also analyzed in the paper. The results showed that the content of O2-and H2O2in bolh poplar cuhivars increased with the time after inoculation. The "oxidative burst" was clear in resistant cultivar and unclear in susceptible one; POD activity increased with a higher level in resistant cultivar than that in the susceptible one at the late stage; SOD and CAT activities increased at first stage and declined later, while the inhibition was more significant io the susceptible one; APX activity changed a lot in two cultivars and variable range was significant in the resistant one. The increased MDA appeared that "oxidative burst" was likely to initiate membrane lipid peroxidation. MDA content has a sharp increase in testing P. beijingensis at late stage after inoculation and a significant positive correlation with the rate of O2: generation.

引用本文

导出引用
王媛1,2,梁军1,张星耀1*. 抗、感病杨树与溃疡病菌互作中活性氧及相关酶的动态[J]. 南京林业大学学报(自然科学版). 2008, 32(05): 41-46 https://doi.org/10.3969/j.jssn.1000-2006.2008.05.008
WANG Yua1,2, LIANG Jan1, ZHANG Xin-yao1*. Changes of active oxygen and related enzymes during the interaction of poplar and canker disease pathogen[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2008, 32(05): 41-46 https://doi.org/10.3969/j.jssn.1000-2006.2008.05.008
中图分类号: S763   

Accesses

Citation

Detail

段落导航
相关文章

/