Abstract: The tender buds of Rhododendron redowskianum Maxim. were used as explants and suitable medium compositions were screened through uniform design experiments. The results showed that DR+TDZ(1.80 mg/L)+IBA(0.05 mg/L) was the most suitable for shoots regeneration immediately at base of tender buds with a regeneration rate of 100%. MS(modified)+IBA(0.08 mg/L)+NAA(0.02 mg/L)+KT(0.15 mg/L) was most suitable for rooting with a rooting rate of 99%. 1/7MS+B9(1.30 mg/L)+Phloridzin(2.80 mg/L) was most suitable for in vitro germplasm preservation under which the plant materials could be maintained for 35 months at normal temperature. Stems each with one node were cut from regenerated shoots and cultured for propagation, and a 55fold proliferation rate was achieved within 35 days. In vitro culture and in vitro germplasm preservation system of Rh.redowskianum Maxim. has been successfully established in this study.