为探讨3种优良外生菌根真菌对不同盐碱土壤的适应性，从我国山东和江苏杨树栽植区分别采集盐碱土壤，对其在盐碱土壤提取液中的生长及离子吸附状况进行了测定。结果表明： 3种外生菌根真菌均生长良好，生物量未受到明显抑制，但对不同地区盐碱土的适应性略有差异。劣味乳菇(编号Li)在3种盐碱土壤中均能良好生长，表明其适应性广泛；红绒盖牛肝菌(编号Xc)较适应在山东德州和东营盐碱土壤中生长；美味牛肝菌(编号Be)则在江苏盐城盐碱土壤中生长较好。在3种外生菌根真菌中，Xc和Li具有较强的Na+和K+吸收能力，为吸盐型菌株；而Be抑制Na+的吸收，为拒盐型菌株。这些表明3种外生菌根真菌均具有较强的耐盐碱能力，而其耐盐碱方式不同。
Abstract
In order to select the excellent ectomycorrhizal fungi from three species of ectomycorrhizal fungi adapting to different saline areas, three species of ectomycorrhizal fungi were grown in vitro in the saline soil extracts, which were collected from poplarplantations in Shandong and Jiangsu province. The colony diameter, biomass and salt ions content of fungi were evaluated. The results showed that the three species of ectomycorrhizal fungi had greater capacities of saline tolerance. Their growth and biomass were not obviously reduced under salt stress, but they were adapted to different saline area. Lactaricu insulsus (Li) grew well in all saline area, so it had wide range of adaptability. Xerocamus chrysenteron (Xc) grew well in saline area of Dezhou and Dongying. Boletus edulis (Be) grew well in saline area of Yancheng only. Li and Xc exhibited greater capacities of Na+ and K+ absorption under salt stress, so they were saltincluder; Be exhibited greater capacity to exclude Na+, so it was a saltexcluder. We propose that the three species of ectomycorr
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参考文献
[1]弓明钦,陈应龙,仲崇禄,等. 菌根研究及应用[M]. 北京:中国林业出版社,1997.
[2]吴小芹,马磊. 外生菌根真菌产生植物激素差异及其与NL895杨生长的关系[J]. 林业科学,2008,44(7):43-49
[3]王幼珊,张美庆,张弛,等. VA菌根真菌抗盐碱菌株的筛选[J]. 土壤学报,1994,31(增刊):79-83.
[4]Van D W E, Rozema J, Ernst W H O. Seasonal and spatial variation in the occurrence of vesiculararbuscular(VA)mycorrhiza in salt marsh plants[J]. Agriculture, Ecosystems and Environment, 1989, 29: 107-110.
[5]Cooke J C, Lefor M W. Comparison of vesiculararbuscular mycorrhizae in plants from disturbed and adjacent undisturbed regions of a coastal salt marsh in Clinton, Connecticut[J]. Environmental Management,1990,14: 131-137.
[6]Sengupta A, Chaudhuri S. Vesiculararbuscular mycorrhiza(VAM)in pioneer salt marsh plants of the Ganges river delta in West Bengal(India)[J]. Plant and Soil, 1990, 122: 111-113.
[7]宋微,吴小芹. 12种林木外生菌根真菌的培养条件[J]. 南京林业大学学报:自然科学版,2007,31(3):133-135.
[8]中华人民共和国林业部科技司. 林业标准汇编:三[M]. 北京:中国林业出版社,1991.
[9]Chen S, Li J, Wang S. Salt nutrient uptake and transport and ABA of Populus euphratica; a hybrid in response to increasing soil NaCl[J]. Trees,2001,15: 186-194
[10]Nagarajan G, Natarajan K. The use of BoxBehnken design of experiments to study in vitro salt tolerance by Pisolthus tinctorius[J]. World J Microbiol Biotech, 1999, 15(2): 197-200.
[11]Ding R K, Rao M V, Garg V K. Salt stress affects in vitro growth and situ symbioses of ectmycorrhizal fungi[J]. Mycorrhiza,1993, 3: 63-68
[12]Zhong H, Li j, Chen S L. Effect of NaCl on growth and ion relation in two salttolerant strains of Paxillus involutus[J]. For Std China, 2008, 10(2): 95-100.
[13]姜学艳,黄艺. 菌根真菌增加植物抗盐碱胁迫的机理[J]. 生态环境,2003,12(3):353-356.
[14]LangenfeldHeyser R,Gao J, Ducic T, et al. Paxillus involutus mycorrhiza attenuate NaClstress responses in the saltsensitive hybrid poplar Populus × canescens[J]. Mycorrhiza, 2007, 17: 12 1-13
[15]Muhsin T M, Zwiazek J J. Colonization with Hebeloma crustuliniforme increases water conductance and limits shoot sodium up take in white spruce (Picea glauca) seedlings[J]. Plant Soil, 2002, 38: 217-225.
基金
收稿日期：2008-09-24修回日期：2009-03-23基金项目：国家自然科学基金资助项目(30571471)；江苏省重大科技支撑与自由创新专项(BE2008393)作者简介：范克胜(1980—)，博士生。﹡吴小芹(通讯作者)，教授，研究方向为森林保护学。Email: xqwu@njfu.com.cn。引文格式：范克胜,吴小芹. 3种外生菌根真菌在杨树栽植区盐碱土壤中的适应性分析[J]. 南京林业大学学报:自然科学版,2009,33(4):53-56.
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