汽爆玉米秸秆渣诱导产纤维素酶及其水解特性

欧阳嘉; 董郑伟; 谢喆; 李鑫; 宋向阳

doi:10.3969/j.jssn.1000-2006.2009.04.020

欧阳嘉，董郑伟，谢喆，李鑫，宋向阳

南京林业大学学报（自然科学版） ›› 2009, Vol. 33 ›› Issue (04) : 96-100.

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南京林业大学学报（自然科学版） ›› 2009, Vol. 33 ›› Issue (04) : 96-100. DOI: 10.3969/j.jssn.1000-2006.2009.04.020

研究论文

汽爆玉米秸秆渣诱导产纤维素酶及其水解特性

欧阳嘉，董郑伟，谢喆，李鑫，宋向阳

作者信息 +

Characteristics of cellulase inducement and hydrolysis by steam exploded corn stover

OUYANG Jia, DONG Zhengwei, XIE Zhe, LI Xin, SONG Xiangyang

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摘要

为提高玉米秸秆的生物降解效率，研究了汽爆处理玉米秸秆渣对纤维素酶的诱导作用及所产纤维素酶的水解特性。结果表明：玉米秸秆采用汽爆处理可以去除大量的半纤维素成分，获得的汽爆渣对里氏木霉NL02合成纤维素酶的诱导作用良好，以10 g/L的汽爆渣为碳源发酵7 d滤纸酶活达到1.90 U /mL。发酵5 d获得的汽爆酶水解效果最佳，当酶用量25 U/g，底物质量浓度50 g/L条件下，水解24 h酶解得率达到95.9%，水解48 h葡萄糖产率为76.4%。当汽爆渣质量浓度为80 g/L，酶用量为15 U/g时，48 h酶解效率达到90.1%，葡萄糖产率为62.9%。

Abstract

The characteristics of cellulase inducement and hydrolysis by steam exploded corn stover were investigated. Steam explosion could remove hemicellulose from corn stover and the acquired residue was a good inducer for cellulase biosynthesis using Trichoderma reesei NL02. When the concentration of substrate was 10 g/L, the filter paper activity reached 1.90 U/mL after cultivation for seven days. The four produced and the two other enzymes were characterized by various enzyme activity measurements and steam exploded corn stover was hydrolyzed by them. The result showed that the highest sugar yields were obtained with the enzyme induced by steam exploded corn stove after cultivating for five days. When 50 g/L steam exploded residue was treated with the enzyme dosage of 25 U/g, the enzymatic hydrolysis yield was 95.9% for 24 h and the glucose yield was 76.4% for 48 h, and when the substrate concentration was improved to 80 g/L with the dosage of 15 U/g, the hydrolysis yield was 90.1% and the glucose yield was 62.9% for 48 h under the same conditions.

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欧阳嘉，董郑伟，谢喆，李鑫，宋向阳. 汽爆玉米秸秆渣诱导产纤维素酶及其水解特性[J]. 南京林业大学学报（自然科学版）. 2009, 33(04): 96-100 https://doi.org/10.3969/j.jssn.1000-2006.2009.04.020

OUYANG Jia, DONG Zhengwei, XIE Zhe, LI Xin, SONG Xiangyang. Characteristics of cellulase inducement and hydrolysis by steam exploded corn stover[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2009, 33(04): 96-100 https://doi.org/10.3969/j.jssn.1000-2006.2009.04.020

中图分类号： TQ352

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基金

收稿日期：2008-07-28修回日期：2008-12-12基金项目：国家高技术研究发展计划(2007AA02Z213；2006AA020204)；国家重点基础研究发展计划(2007CB707801)作者简介：欧阳嘉(1972—) ，副教授，博士，研究方向为酶工程及纤维资源利用。Email: Jia_ouyang72@hotmail.com。引文格式：欧阳嘉,董郑伟,谢喆,等. 汽爆玉米秸秆渣诱导产纤维素酶及其水解特性[J]. 南京林业大学学报:自然科学版,2009,33(4):96-100.