Abstract: The effects of different degrees of periodical drought stress on soluble sugar content in needles of Pinus massoniana seedlings from five different provenances which were Lechang, Guangdong province, Yuan’an, Hubei province, Rucheng, Hunan province, Jiangshan, Zhejiang province, Huoshan, Anhui province were studied. The results showed that: drought stress could obviously affect the contents of mannose, glucose, galactose and maltose in needles of P.massoniana seedlings. The accumulation of mannose generally increased along with the prolonging of stress. Compared with 12 days stress, the mannose content of control increased by 37.75% after 36 days stress, that of medium and severe drought stress increased by 59.07% and 60.95% respectively. The content of glucose did not change regularly in the first two cycles of drought stress but it would be obviously different between control and drought stress after three cycles treatment. The content of galactose was unstable and was not significantly different among different provenances in the beginning of drought stress, but it would steadily increase during prolonging of drought stress. The content of maltose had a trend to decrease during the whole period of drought stress. In general, the contents of above four sugars accumulated in the seedlings of the provenance from Huoshan, Anhui province were the highest, followed by provenance from Lechang, Guandong province. And the lowest contents of sugars were observed in the seedlings of provenances from Yuan’an, Hubei province, Jiangshan, Zhejiang province and Rucheng, Hunan province.