应用扫描电镜对蜡梅3个品种群共21个品种的花粉粒形态特征进行了观察和比较,对观测结果进行了主成分分析与聚类分析。结果表明:蜡梅花粉粒为中等大小,近扁球形至圆球形;蜡梅花粉外壁纹饰可分为蠕虫状和拟蠕虫状两个类型;蜡梅品种间花粉形态比较类似,但在纹饰细微特征、花粉粒大小、萌发沟长宽度等方面存在一定差异,可作为区分品种的辅助依据;花粉形态聚类分析结果与传统形态分类结果存在差异,花粉形态与内被片和中被片颜色并无直接关联。
Abstract
Pollen grain characteristics of 21 Chimonanthus praecox cultivars in 3 groups were examined and compared under scanning electron microscope (SEM), and principal component analysis and cluster analysis were conducted based on observation results. The results showed that the pollen grain was in midsize, and in suboblate to globular shape. The exine ornamentation could be divided into two types: helminthoid and quasihelminthoid. Pollen morphology of the cultivars was quite similar, but there were certain differences in details of ornamentation, size of pollen grains, length and width of colpus, so they could provide ancillary evidence for cultivar identification. There was difference between the result of cluster analysis for pollen morphology and that of traditional morphological classification, there was no direct relationship between pollen morphology and color of inner or medium petals.
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
[1]赵天榜. 中国蜡梅[M]. 郑州: 河南科学技术出版社,1991.
[2]陈龙清,赵凯歌,周明芹. 蜡梅品种分类体系探讨[J]. 北京林业大学学报,2004,26(S1):88-90.
[3]程红梅. 江苏地区蜡梅品种资源调查研究[D]. 南京:南京林业大学,2005.
[4]孙钦花. 南京地区蜡梅品种资源调查及分类研究[D]. 南京:南京林业大学,2007.
[5]Erdtman G. 孢粉学手册[M]. 中国科学院植物所,译.北京:科学出版社,1978.
[6]王开发,王宪曾. 孢粉学概论[M]. 北京:北京大学出版社,1983.
[7]张若蕙,张金谈,郝海平. 蜡梅科的花粉形态及其系统位置的探讨[J]. 浙江林学院学报,1989,6(1):1-8.
[8]李林初. 夏蜡梅属花粉形态的研究[J]. 植物研究,1990,10(1):93-98.
[9]康素红,包满珠,陈龙清,等. 梅花品种分类的花粉形态学研究[J]. 园艺学报,1997,24(2):170-174.
[10]方从兵,盛炳成,章镇. 花粉形态特征与梅品种分类研究[J]. 安徽农业大学学报,2002,29(2):137-142.
[11]金飚,何小弟,吴建华,等. 芍药花粉的形态特征及其与品种演化的关系[J]. 江苏农业学报,2005,21(1):63-68.
[12]臧德奎. 桂花品种分类研究[D]. 南京:南京林业大学,2004.
[13]张秀英,王雁. 桃花种质资源花粉形态的观察与比较[J]. 北京林业大学学报,1997,19(2):57-62.
[14]王奎玲,刘庆超,黄鑫,等. 耐冬山茶孢粉学研究[J]. 中国农学通报,2007,23(11):267-272.
[15]李玉舒,张启翔. 中国玫瑰品种资源花粉形态的观察与比较[J]. 北方园艺,2009(8):179-182.
基金
收稿日期:2009-05-24修回日期:2010-03-15作者简介:芦建国(1960—),教授。Email: ljgnj@sina.com。引文格式:芦建国,熊钢. 蜡梅品种花粉粒扫描电镜特征的分类研究[J]. 南京林业大学学报:自然科学版,2010,34(4):145-148.