韩国伞滑刃属线虫的形态和分子特征

doi:10.3969/j.jssn.1000-2006.2011.01.033

韩国伞滑刃属线虫的形态和分子特征

南京林业大学学报（自然科学版） ›› 2011, Vol. 35 ›› Issue (01) : 142-142.

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南京林业大学学报（自然科学版） ›› 2011, Vol. 35 ›› Issue (01) : 142-142. DOI: 10.3969/j.jssn.1000-2006.2011.01.033

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韩国伞滑刃属线虫的形态和分子特征

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Morphology and molecular characterization of Bursaphelenchus(Nematoda: Aphelenchoididae) spp. distributed in KoreaHyerim

Hyerim Han, Bo Young Han, Yeong Jin Chung, Sang Chul Shin

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摘要

以锥尾伞滑刃线虫（Bursaphelenchus conicaudatus）作为对照，在韩国不同地域和寄主中采集了15个松材线虫分离物、3 个拟松材线虫的分离物及5个未鉴定的该属的分离物,并对其进行了ITS 和 D2D3 rDNA序列分析。以单条雌线虫的DNA为模板,ITS 和 D2D3 区由PCR仪扩增并克隆其序列。结果表明,所有松材线虫的ITS 和 D2D3 区序列相同，没有种内变异。然而2个采自黑松和红松的拟松材线虫分离物的基因型分别是亚洲型和欧洲型。5个未鉴定线虫的数据表明，它们分别接近B. tusciae、B. lini、B. thailandae、B. doui和 B. hylobianum,该结果同时被形态学结果所支持。利用5种酶（Hinf I、Alu I、Msp I、Hae III、Rsa）,PCR产物克隆并测序的数据也可以区分不同种和基因型。

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韩国伞滑刃属线虫的形态和分子特征[J]. 南京林业大学学报（自然科学版）. 2011, 35(01): 142-142 https://doi.org/10.3969/j.jssn.1000-2006.2011.01.033

Hyerim Han, Bo Young Han, Yeong Jin Chung, Sang Chul Shin. Morphology and molecular characterization of Bursaphelenchus(Nematoda: Aphelenchoididae) spp. distributed in KoreaHyerim[J]. Journal of Nanjing Forestry University (Natural Sciences Edition）. 2011, 35(01): 142-142 https://doi.org/10.3969/j.jssn.1000-2006.2011.01.033

中图分类号： S763

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