聚苯乙烯微球中荧光共振能量转移的研究

doi:10.3969/j.jssn.1000-2006.2011.02.018

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南京林业大学学报（自然科学版） ›› 2011, Vol. 35 ›› Issue (02): 83-87.doi: 10.3969/j.jssn.1000-2006.2011.02.018

聚苯乙烯微球中荧光共振能量转移的研究

吴伟兵¹，王明亮²，景宜¹，戴红旗¹，周小凡¹

1.南京林业大学，江苏省制浆造纸科学与技术重点实验室，江苏南京210037； 2.东南大学化学化工学院，江苏南京211189

出版日期:2011-04-13 发布日期:2011-04-13
基金资助:
收稿日期：2010-01-26修回日期：2010-06-01 基金项目：江苏省制浆造纸科学与技术重点实验室开放课题（200907）；国家自然科学基金项目（30972321）作者简介：吴伟兵(1982—)，讲师，博士。Email: wbwu@yahoo.cn。引文格式：吴伟兵，王明亮，景宜,等. 聚苯乙烯微球中荧光共振能量转移的研究[J]. 南京林业大学学报:自然科学版,2011,35(2):83-87.

Study on fluorescence resonance energy transfer in polystyrene microspheres microspheres

WU Weibing¹， WANG Mingliang²， JING Yi¹ ，DAI Hongqi¹， ZHOU Xiaofan¹

1. Jiangsu Provincial Key Lab of Pulp and Paper Science and Technology, Nanjing Forestry University, Nanjing 210037, China; 2. School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, China

Online:2011-04-13 Published:2011-04-13

摘要/Abstract

摘要： 采用分散共聚合法制备了单分散的负载有1, 8-萘酰亚胺染料的荧光微球。利用供体和受体染料间的荧光共振能量转移，荧光微球在激发供体染料时同时实现了供体和受体染料的双重荧光发射信号。结果表明：当微球中染料的浓度相对较低（质量分数小于02%）时，荧光共振能量就可以有效地发生转移。聚合过程中增加供体或受体染料的浓度都会提高能量转移效率，这是浓度增加使得染料分子间距离缩短造成的。通过调整微球中染料浓度可以获得不同能量转移效率、具有荧光编码信号的系列荧光微球。这类粒径均一的荧光微球在多元生物分析中有潜在的应用价值。

Abstract: A series of fluorescent monodisperse polystyrene microspheres labeled with 1, 8naphthamide dyes were prepared by dispersion copolymerization. Making use of fluorescence resonance energy transfer (FRET), the prepared microspheres showed both the fluorescence of the donor and acceptor dyes under the excitation wavelength of the donor dye. It is found that effective FRET occurs at a relatively low dye concentration and appropriate molar ratio of donor and acceptor. An increase in the dye concentration, which reduces the distance between the donor and acceptor, leads to more effective FRET in microspheres. By changing the dye concentrations, microspheres could show tuned fluorescent signals under a single wavelength excitation. This type of uniform fluorescent microspheres has potential application in bioassays.

中图分类号:

Q632，TB34

吴伟兵,王明亮,景宜,等. 聚苯乙烯微球中荧光共振能量转移的研究[J]. 南京林业大学学报（自然科学版）, 2011, 35(02): 83-87.

WU Weibing， WANG Mingliang， JING Yi ，DAI Hongqi， ZHOU Xiaofan. Study on fluorescence resonance energy transfer in polystyrene microspheres microspheres[J].Journal of Nanjing Forestry University (Natural Science Edition), 2011, 35(02): 83-87.DOI: 10.3969/j.jssn.1000-2006.2011.02.018.

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聚苯乙烯微球中荧光共振能量转移的研究

Study on fluorescence resonance energy transfer in polystyrene microspheres microspheres

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