为提高酶解液中聚合度为2～5的低聚木糖含量，采用超滤的方法对木聚糖酶进行分级，用于高温降解木聚糖的酶水解。结果表明：木聚糖酶原酶液经超滤分级后，得到的木聚糖酶A组分（分子质量为30～50 ku）、B组分（分子质量为10～30 ku）和C组分（分子质量小于10 ku），均能使木聚糖中聚合度较高的糖降解为聚合度为2～5的低聚木糖。木聚糖酶B组分由于富含内切木聚糖酶XYN I和XYNⅡ，1 mg酶蛋白可以产生263 g木二糖、185 g木三糖、115g木四糖和046 g木五糖，明显高于木聚糖酶A和C组分高温降解木聚糖的水解能力。
Abstract
In order to increase xylooligosaccharides in the enzymatic hydrolysate with a degree of polymerization(DP) between 2 and 5, crude xylanase was ultrafiltrated to hydrolyze hightemperature degraded xylan The results showed that three xylanase components, named xylanase A (MW 30—50 ku), xylanase B (MW 10—30 ku) and xylanase C (MW＜10 ku), were obtained after ultrafiltration fraction of crude xylanase. These xylanase components were able to hydrolyze the hightemperature degraded xylan efficiently to xylooligosaccharides with a DP between 2 and 5 Xylanase B performed much better than xylanase A and C; 1 mg protein of xylanase B could produce xylobiose 263 g, xylotriose 185 g, xylotetraose 115 g and xylopentaose 046 g
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基金
收稿日期：2010-04-21修回日期：2011-03-18基金项目：江苏省科技支撑计划（BE2010732）;国家林业局林业公益性行业科研专项（200904017）;江苏省高校科技创新团队资助项目作者简介：刘蕾（1982—），讲师，硕士。*余世袁（通信作者）， 教授。Email:syu@njfu.edu.cn。引文格式：刘蕾，勇强，余世袁. 木聚糖酶分级对高温降解木聚糖酶水解的影响［J］. 南京林业大学学报:自然科学版,2011,35(3):106-110.
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