木聚糖酶分级对高温降解木聚糖酶水解的影响

doi:10.3969/j.jssn.1000-2006.2011.03.022

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南京林业大学学报（自然科学版） ›› 2011, Vol. 35 ›› Issue (03): 21-.doi: 10.3969/j.jssn.1000-2006.2011.03.022

木聚糖酶分级对高温降解木聚糖酶水解的影响

刘蕾¹,勇强² ,余世袁²^*

1. 南京化工职业技术学院，江苏南京210048；2. 南京林业大学化学工程学院，江苏南京210037

出版日期:2011-06-13 发布日期:2011-06-13
基金资助:
收稿日期：2010-04-21修回日期：2011-03-18基金项目：江苏省科技支撑计划（BE2010732）;国家林业局林业公益性行业科研专项（200904017）;江苏省高校科技创新团队资助项目作者简介：刘蕾（1982—），讲师，硕士。*余世袁（通信作者），教授。Email:syu@njfu.edu.cn。引文格式：刘蕾，勇强，余世袁. 木聚糖酶分级对高温降解木聚糖酶水解的影响［J］. 南京林业大学学报:自然科学版,2011,35(3):106-110.

Enzymatic hydrolysis of hightemperature degraded xylan with xylanase

LIU Lei1, YONG Qiang2, YU Shiyuan2*

1. Nanjing College of Chemical Technology, Nanjing 210048, China;2. College of Chemical Engineering,Nanjing Forestry University, Nanjing 210037, China

Online:2011-06-13 Published:2011-06-13

摘要/Abstract

摘要： 为提高酶解液中聚合度为2～5的低聚木糖含量，采用超滤的方法对木聚糖酶进行分级，用于高温降解木聚糖的酶水解。结果表明：木聚糖酶原酶液经超滤分级后，得到的木聚糖酶A组分（分子质量为30～50 ku）、B组分（分子质量为10～30 ku）和C组分（分子质量小于10 ku），均能使木聚糖中聚合度较高的糖降解为聚合度为2～5的低聚木糖。木聚糖酶B组分由于富含内切木聚糖酶XYN I和XYNⅡ，1 mg酶蛋白可以产生263 g木二糖、185 g木三糖、115g木四糖和046 g木五糖，明显高于木聚糖酶A和C组分高温降解木聚糖的水解能力。

Abstract: In order to increase xylooligosaccharides in the enzymatic hydrolysate with a degree of polymerization(DP) between 2 and 5, crude xylanase was ultrafiltrated to hydrolyze hightemperature degraded xylan The results showed that three xylanase components, named xylanase A (MW 30—50 ku), xylanase B (MW 10—30 ku) and xylanase C (MW＜10 ku), were obtained after ultrafiltration fraction of crude xylanase. These xylanase components were able to hydrolyze the hightemperature degraded xylan efficiently to xylooligosaccharides with a DP between 2 and 5 Xylanase B performed much better than xylanase A and C; 1 mg protein of xylanase B could produce xylobiose 263 g, xylotriose 185 g, xylotetraose 115 g and xylopentaose 046 g

中图分类号:

刘蕾,勇强,余世袁. 木聚糖酶分级对高温降解木聚糖酶水解的影响[J]. 南京林业大学学报（自然科学版）, 2011, 35(03): 21-.

LIU Lei1, YONG Qiang2, YU Shiyuan2*. Enzymatic hydrolysis of hightemperature degraded xylan with xylanase[J].Journal of Nanjing Forestry University (Natural Science Edition), 2011, 35(03): 21-.DOI: 10.3969/j.jssn.1000-2006.2011.03.022.

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木聚糖酶分级对高温降解木聚糖酶水解的影响

Enzymatic hydrolysis of hightemperature degraded xylan with xylanase

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