从江苏省南京市江宁区、南京市紫金山、镇江市、淮安市、连云港市和扬州市等5个市6个地区采集80份马尾松（Pinus massoniana）、黑松（P. thunbergii）、湿地松（P. elliottii）和赤松（P. densiflora）的根和根际土壤样品，并分离鉴定其中的寄生线虫，初步分析结果如下：从采自南京紫金山上健康的马尾松根际中分离得到双宫螺旋线虫（Helicotylenchus dihystera）和松拟盘旋线虫（ Paratylenchus pini）；从南京江宁区衰弱马尾松根际中分离得到斯氏细小线虫（Gracilacus steineri）、尖头细小线虫（G. epacris）和斯特林细小线虫（G. straeleni），从健康马尾松根际中分离得到布科文针线虫（ P. bukowinensis）；从扬州市健康的马尾松根际中分离得到苹果矮化线虫（Tylenchorhynchus malinus）；从镇江市健康黑松根际中分离得到微小针线虫（P. minutus）。其中，马尾松是苹果矮化线虫、布科文针线虫、斯特林细小线虫、尖头细小线虫和斯氏细小线虫的新寄主，黑松是微小针线虫的新寄主。同时，布科文针线虫、斯特林细小线虫、尖头细小线虫和斯氏细小线虫为江苏省的新纪录种。
Abstract
Isolation and species identification of the rhizosphere parasitic nematodes were carried out from 80 roots and soil samples, which were collected from Pinus massoniana, P. thunbergii, P. elliottii, P. densiflora from Nanjing, Zhenjiang, Huaian, Lianyungang and Yangzhou cities in Jiangsu province. The results were as follows：Helicotylenchus dihystera and Paratylenchus pini were isolated from the rhizosphere of healthy P. massoniana grown in Mount Zijin of Nanjing City; Gracilacus steineri, G. epacris and G. straeleni were found in the rhizosphere of weak P. massoniana in Jiangning district of Nanjing City; Tylenchorhynchus malinus were observed in the rhizosphere of healthy P. massoniana in Yangzhou City; P. minutus were from healthy P. thunbergii in Zhenjiang City. P. massoniana was a new host of T. malinus, P. bukowinensis, G. straeleni, G. epacris and G. steineri, while P. thunbergii was a new host of P. minutus. Moreover, P. bukowinensis, G. steineri, G. epacris and G. straeleni were the new record species in Jiangsu province.
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基金
收稿日期：2011-11-30修回日期：2012-05-09
基金项目：国家自然科学基金项目（31170606）
第一作者：方爱琴，硕士生。*通信作者：谈家金，副教授。E-mail: tanjiajin@tom.cn。
引文格式：方爱琴，谈家金，叶建仁,等. 江苏省松树根际寄生线虫种类鉴定[J]. 南京林业大学学报:自然科学版,2012,36(5):109-114.
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