以青钱柳幼胚为外植体，研究了不同幼胚期、基本培养基种类、植物生长调节剂种类及其浓度配比对愈伤组织诱导的影响。结果表明，不同发育期的幼胚诱导出的愈伤组织在形态上差异较大，青钱柳种子发育至子叶胚中期较适合愈伤组织的诱导。青钱柳幼胚愈伤诱导时基本培养基以改良DKW效果最佳，诱导率达到了88.89 %，且无褐化现象；含2,4-D与KT组合的培养基较适宜愈伤组织的诱导，其中2,4-D（0.5 mg/L）+KT （2.0 mg/L）和2,4-D （1.0 mg/L）+KT （1.0 mg/L）的组合对青钱柳幼胚诱导愈伤组织效果最佳，诱导率分别为80.00 %和78.33 %。
Abstract
Using young embryos of Cyclocarya paliurus as explants, we investigated the effects of embryo development period, basic media, types and the concentrations of plant growth regulators on callus induction of C. paliurus. The results showed that there were obvious differences in the shape of callus among different embryo development periods, and the middle cotyledon embryos were most suitable for callus induction. As a basic media, improved DKW medium was the best selection for young embryo callus induction, of which induction rate could hit to 88.89 % with no browning phenomenon. The medium containing 2,4-D and KT was more appropriate for callus induction. The 2,4-D (0.5 mg/L)+KT (2.0 mg/L) as well as 2,4-D (1.0 mg/L)+KT (1.0 mg/L) were optimal for callus induction of C. paliurus young embryo, and the induction rates were 80.00 % and 78.33 % respectively.
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
[1]中国科学院中国植物志编辑委员会.中国植物志：21卷[M].北京:科学出版社,1979.
[2]方升佐,杨万霞.青钱柳的开发利用与资源培育[J].林业科技开发,2003,17(1):49-51.
[3]Xie M Y, Li L. Review in studies on chemical constituents and bioactivities of Cyclocarya paliurus[J]. Chinese Traditional and Herbal Drugs,2001,32(4):365-366.
[4]舒庆庚,徐昌瑞，黎莲娘.青钱柳甜味成分的研究[J].药学学报,1995,30(10):757-761.
[5]冷任轩.青钱柳叶的基础理论研究和临床观察[J].江西中医药,1994,25(2):64-65.
[6]Kurihara H, Asami S, Shibata H, et al. Hypolipemic effect of Cyclocarya paliurus (Batal.) Iljinskaja in lipidloaded mice[J]. Biological and Pharmaceutical Bulletin,2003,26(3):383-385.
[7]Kurihara H, Fukami H, Kusumoto A, et al. Hypoglycaemic action of Cyclocarya paliurus (Batal.) Iljinskaja in normal and diabetic mice[J]. Bioscience, Biotechnology and Biochemistry,2003,67(4):877-880.
[8]Jiang Z Y, Zhang X M, Zhou J, et al. Two new triterpenoid glycosides from Cyclocarya paliurus[J]. Journal of Asian Natural Products Research,2006,8(1-2):93-98.
[9]Xie M Y, Li L, Nie S P, et al. Determination of speciation of elements related to blood sugar in bioactive extracts from Cyclocarya paliurus leaves by FIAICPMS[J]. European Food Research and Technology,2006,223(2): 202-209.
[10]李磊,赵丽,谢明勇.青钱柳多糖组分及其降血糖活性研究[J].江西农业大学学报,2001,23(4):484-486.
[11]洪俊溪.青钱柳人工林材性试验的研究[J].福建林学院学报,1997,17(3):214-217.
[12]方升佐,洑香香.青钱柳资源培育与开发利用的研究进展[J].南京林业大学学报:自然科学版,2007,31(1):95-100.
[13]上官新晨,郭春兰,蒋艳,等.培养基和植物激素对青钱柳茎段和叶片愈伤组织诱导的研究[J].江西农业大学学报,2006,28(5):678-682.
[14]乔卿梅,程茂高,王新民,等.青钱柳离体胚的组织培养研究[J].广西农业科学,2009(7):68-71.
[15]尚旭岚,徐锡增,方升佐.青钱柳离体胚的培养及快速繁殖[J].南京林业大学学报:自然科学版,2007,31(1):101-105.
[16]谢寅峰,王莹,尚旭岚，等.青钱柳组培快繁体系的初步研究[J].西北植物学报,2009,29(11):2331-2338.
[17]徐叔文,汤章城.植物生理与分子生物学[M].2版.北京:科学出版社,1998.
[18]胡冬南,蒋艳,吴少福,等.青钱柳组织培养的初步研究[J].江西农业大学学报,2005,27(1):39-41.
[19]王玉珍,罗景兰,徐进,等.农杆菌介导的水稻遗传转化与植株再生[J].华北农学报,2005,20(2):8-11.
[20]李玉静,陈彦龙,王玲玲,等.2,4-D和6-BA对水稻愈伤组织培养力的影响[J].河北师范大学学报:自然科学版,2005,29(4):395-403.
基金
收稿日期：2011-11-17修回日期：2012-04-25
基金项目：江苏高校优势科技创新团队科研计划（苏教科[2009]10号）；国家林业局林业科学技术推广项目﹙[2011]18号）；国家林业公益性行业科研专项项目（200904046）
第一作者：张金凤，硕士生。*通信作者：方升佐，教授。E-mail: fangsz@njfu.edu.cn。
引文格式：张金凤，方升佐，尚旭岚,等. 青钱柳幼胚愈伤组织的诱导[J]. 南京林业大学学报:自然科学版,2012,36(5):47-50.
PDF(738942 KB)
