野百合与金佛山百合的核型分析及45S rDNA 在其染色体上的物理定位

席梦利,房磊,张晓玲,辛昊阳,高婷婷,施季森

南京林业大学学报(自然科学版) ›› 2013, Vol. 37 ›› Issue (06) : 153-156.

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南京林业大学学报(自然科学版) ›› 2013, Vol. 37 ›› Issue (06) : 153-156. DOI: 10.3969/j.issn.1000-2006.2013.06.030
研究简报

野百合与金佛山百合的核型分析及45S rDNA 在其染色体上的物理定位

  • 席梦利,房 磊,张晓玲,辛昊阳,高婷婷,施季森
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The karyotype analysis and physical localization of 45S rDNA on the chromosomes of Lilium brownii and L.jinftishanense

  • XI Mengli, FANG Lei, ZHANG Xiaoling, XIN Haoyang, GAO Tingting, SHI Jisen
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摘要

采用涂片法和45S rDNA荧光原位杂交技术,对野百合(Lilium brownii)和金佛山百合(L.jinftishanense)进行了核型分析和45S rDNA在其染色体上的物理定位。核型分析结果表明:野百合的核型为3B,金佛山百合核型为3A; 野百合的核型公式为2n=2x=24=4m+8st(4SAT)+12t,金佛山百合的核型公式为2n=2x=24=4m+8st+12t; 野百合和金佛山百合的核不对称系数分别为82.12%和82.65%。荧光原位杂交结果表明:野百合有6个45S rDNA位点,它们分别位于3、6、9号染色体上; 金佛山百合有8个45S rDNA位点,它们分别位于1、3、6、8号染色体上。

Abstract

The karyotype analysis and physical localization of 45S rDNA on the chromosomes of Lilium brownii and L.jinftishanense were studied by using the smear methods and the technology of 45S rDNA fluorescence in situ hybridization. The result of karyotype analysis indicated that the karyotype of L.brownii was 3B, the karyotype of L.jinftishanense was 3A; The karyotype formula was 2n=2x=24=4m+8st(4SAT)+12t for L.brownii, 2n=2x=24=4m+8st+12t for L.jinftishanense; As.k was 82.12% and 82.65%respectively. The result of fluorescence in situ hybridization showed that L.brownii had 6 loci of 45S rDNA distributed on chromosome No.3, No.6 and No.9 and L.jinftishanense had 8 on No.1, No.3, No.6 and No.8, respectively.

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席梦利,房磊,张晓玲,辛昊阳,高婷婷,施季森. 野百合与金佛山百合的核型分析及45S rDNA 在其染色体上的物理定位[J]. 南京林业大学学报(自然科学版). 2013, 37(06): 153-156 https://doi.org/10.3969/j.issn.1000-2006.2013.06.030
XI Mengli, FANG Lei, ZHANG Xiaoling, XIN Haoyang, GAO Tingting, SHI Jisen. The karyotype analysis and physical localization of 45S rDNA on the chromosomes of Lilium brownii and L.jinftishanense[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2013, 37(06): 153-156 https://doi.org/10.3969/j.issn.1000-2006.2013.06.030
中图分类号: Q948   

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基金

收稿日期:2012-06-05 修回日期:2013-09-30
基金项目:江苏省农业科技自主创新资金项目(CX(12)2018); 国家林业局“948”项目(2013-4-34)
第一作者:席梦利,副教授。E-mail: ximenglinjfu@126.com。
引文格式:席梦利,房磊,张晓玲,等. 野百合与金佛山百合的核型分析及45S rDNA在其染色体上的物理定位[J]. 南京林业大学学报:自然科学版,2013,37(6):153-156.

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