橡胶树胶乳橡胶粒子死皮相关蛋白的鉴定及分析

doi:10.3969/j.issn.1000-2006.2014.01.007

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南京林业大学学报（自然科学版） ›› 2014, Vol. 38 ›› Issue (01): 36-40.doi: 10.3969/j.issn.1000-2006.2014.01.007

橡胶树胶乳橡胶粒子死皮相关蛋白的鉴定及分析

袁坤¹,周雪梅^1,2,王真辉¹,杨礼富^1*

1.中国热带农业科学院橡胶研究所,农业部橡胶树生物学与遗传资源利用重点实验室,海南儋州 571737;
2.海南大学农学院,海南海口 570100

出版日期:2014-01-15 发布日期:2014-01-15
基金资助:
收稿日期:2013-03-13 修回日期:2013-07-10
基金项目:中央级公益性科研院所基本业务费专项项目(YWFZX2008-12,2009-12,2010-9,1630022013003); 海南省重点科技项目(90107); 现代农业产业技术体系建设专项资金项目(CARS-34-02A)
第一作者:袁坤,副研究员。*通信作者:杨礼富,研究员。E-mail:ylfri@126.com
引文格式:袁坤,周雪梅,王真辉,等. 橡胶树胶乳橡胶粒子死皮相关蛋白的鉴定及分析[J]. 南京林业大学学报:自然科学版,2014,38(1):36-40.

Identification and analysis of latex rubber particle proteins related with tapping panel dryness in Hevea brasiliensis

YUAN Kun¹, ZHOU Xuemei^1,2, WANG Zhenhui¹, YANG Lifu^1*

1.Rubber Research Institute, Key Laboratory of Biology and Genetic Resources of Rubber Tree, Ministry of Agriculture, Danzhou 571737, China;
2.College of Agriculture, Hainan University, Haikou 570100, China

Online:2014-01-15 Published:2014-01-15

摘要/Abstract

摘要： 橡胶树死皮是影响天然橡胶产量的重要因子之一。采用双向凝胶电泳对健康树和死皮树橡胶粒子中差异表达的蛋白进行分离,分析获得35个差异表达的蛋白点,通过基质辅助激光解析电离飞行时间质谱(MALDI-TOF MS)分析及搜索Uniprot rubber数据库后,有13个蛋白点被成功鉴定,其中橡胶延长因子(rubber elongation factor,REF)、法尼基焦磷酸合成酶(Ffarnesyl-diphosphate synthase,FPS)、谷胱甘肽过氧化酶(glutathione peroxidase,GPX)、谷胱甘肽还原酶(glutathione reductase,GR)、翻译控制肿瘤蛋白(translationally controlled tumor protein,TCTP)、热激蛋白(heat shock protein, HSP)等主要蛋白参与了橡胶的生物合成、活性氧代谢及细胞凋亡过程。说明橡胶的生物合成、活性氧代谢及细胞凋亡途径可能是橡胶树死皮发生的关键调控途径。

Abstract: The tapping panel dryness(TPD)in Hevea brasiliensis is one of the important factors influencing natural rubber production. In order to distinguish and identify the differentially expressed proteins of latex rubber particles in healthy and TPD trees, the two-dimensional electrophoresis(2-DE)was used. 35 differentially expressed protein spots were obtained by software analysis. Subjecting to MALDI-TOF MS following by searching for Uniprot rubber database, 13 protein spots were successfully identified, Of them, REF, FPS, GPX, GR, TCTP and HSP were mainly involed in the rubber biosynthesis, reactive oxygen metabolism and cell apoptosis processes. This study further showed that the pathways of the rubber biosynthesis, reactive oxygen metabolism and cell apoptosis were possibly the key regulated ones of TPD and which would provide a theoretical basis for elucidating the TPD mechanism in Hevea brasiliensis.

中图分类号:

S794.1

袁坤,周雪梅,王真辉,等. 橡胶树胶乳橡胶粒子死皮相关蛋白的鉴定及分析[J]. 南京林业大学学报（自然科学版）, 2014, 38(01): 36-40.

YUAN Kun, ZHOU Xuemei, WANG Zhenhui, YANG Lifu. Identification and analysis of latex rubber particle proteins related with tapping panel dryness in Hevea brasiliensis[J].Journal of Nanjing Forestry University (Natural Science Edition), 2014, 38(01): 36-40.DOI: 10.3969/j.issn.1000-2006.2014.01.007.

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橡胶树胶乳橡胶粒子死皮相关蛋白的鉴定及分析

Identification and analysis of latex rubber particle proteins related with tapping panel dryness in Hevea brasiliensis

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