植物胚拯救技术的影响因素及其应用

张倩云; 王莹; 杜玉梁; 尹增芳

doi:10.3969/j.issn.1000-2006.2014.05.028

植物胚拯救技术的影响因素及其应用

张倩云,王莹,杜玉梁,尹增芳

南京林业大学学报（自然科学版） ›› 2014, Vol. 38 ›› Issue (05) : 143-148.

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南京林业大学学报（自然科学版） ›› 2014, Vol. 38 ›› Issue (05) : 143-148. DOI: 10.3969/j.issn.1000-2006.2014.05.028

综合述评

植物胚拯救技术的影响因素及其应用

张倩云¹,王莹²,杜玉梁¹,尹增芳^1*

作者信息 +

Influence factors and application of plant embryo rescue techniques

ZHANG Qianyun¹, WANG Ying², DU Yuliang¹, YIN Zengfang^1*

Author information +

文章历史 +

摘要

胚拯救技术在拯救败育胚、阻止胚早期退化和培育新三倍体及多倍体等种质资源方面具有重要作用。植物胚拯救方法主要有3种:胚的活体转移、胚的活体培养及胚的离体培养。在植物胚的离体培养过程中,依据需拯救的植物胚胎发育程度,建立了离体子房培养、胚珠培养和幼胚培养3种培养方式。大量研究结果表明,胚龄、培养基种类及相态、培养条件、渗透压、生长调节剂、外源添加剂和基因型等均会影响植物胚拯救的成败,因而胚拯救技术作为传统育种方法的辅助手段,在突破拯救的胚龄极限、提高植株再生率等方面还有进一步发展的空间。此外,结合胚拯救技术和分子生物学的研究手段,有望通过遗传转化稳定不同特性的新种质,创新植物种质资源。

Abstract

Embryo rescue techniques play an important role in saving abortive embryos, preventing embryo degeneration at early development stage and developing new triploid and polyploid. There were three main methods of plant embryo rescue: in vivo embryo transfer, embryo in vivo culture and embryo in vitro culture. In the process of plant embryo in vitro culture,according to the development degree of the plant embryo which was needed to save, three training modes were set up,that is, in vitro ovary culture, ovule culture and immature embryo culture. A plenty of researches have been shown that embryo age, medium and medium phase, culture conditions, osmotic pressure, growth regulator, complementary materials, genotype and so on, played a great role in the success of embryo rescue. Therefore, embryo rescue techniques as an adjunct to conventional breeding methods will have further room for improvement in such aspects as embryonic age limit and plant regeneration rate. In addition, combining embryo rescue techniques with molecular biological research methods, genetic transformation is expected to stabilize different characteristics of the new germplasm, and innovate the plant resources.

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张倩云,王莹,杜玉梁,尹增芳. 植物胚拯救技术的影响因素及其应用[J]. 南京林业大学学报（自然科学版）. 2014, 38(05): 143-148 https://doi.org/10.3969/j.issn.1000-2006.2014.05.028

ZHANG Qianyun, WANG Ying, DU Yuliang, YIN Zengfang. Influence factors and application of plant embryo rescue techniques[J]. Journal of Nanjing Forestry University (Natural Sciences Edition）. 2014, 38(05): 143-148 https://doi.org/10.3969/j.issn.1000-2006.2014.05.028

中图分类号： Q944

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基金

收稿日期:2013-09-09 修回日期:2014-03-24
基金项目:江苏高校优势学科建设工程资助项目(PAPD); 浙江省重中之重林学一级学科开放基金项目(KF201323)
第一作者:张倩云,硕士生。*通信作者:尹增芳,教授。E-mail: zfyin@njfu.edu.cn。
引文格式:张倩云,王莹,杜玉梁,等. 植物胚拯救技术的影响因素及其应用[J]. 南京林业大学学报:自然科学版,2014,38(5):143-148.