吡咯伯克霍尔德氏菌JK-SH007分批发酵
动力学模型的构建

李浩; 任嘉红; 叶建仁; 王小月

doi:10.3969/j.issn.1000-2006.2016.02.029

吡咯伯克霍尔德氏菌JK-SH007分批发酵动力学模型的构建

李浩,任嘉红1,3,叶建仁,王小月

南京林业大学学报（自然科学版） ›› 2016, Vol. 40 ›› Issue (02) : 173-178.

PDF(2283139 KB)

国家林草科技领军期刊
中国精品科技期刊
中国高校百佳科技期刊
江苏省新闻出版政府奖期刊奖
RCCSE林学权威期刊（A+）
CSCD核心期刊
Scopus数据库收录期刊
中文核心期刊
SCD核心期刊

作者加群：102861116

微信公众号：南京林业大学学报

高级检索

PDF(2283139 KB)

南京林业大学学报（自然科学版） ›› 2016, Vol. 40 ›› Issue (02) : 173-178. DOI: 10.3969/j.issn.1000-2006.2016.02.029

研究简报

吡咯伯克霍尔德氏菌JK-SH007分批发酵动力学模型的构建

李浩^{1, 2},任嘉红^{1, 3},叶建仁^1*,王小月¹

作者信息 +

Construction of batch fermentation kinetics model of Burkholderia pyrrocinia strain JK-SH007

LI Hao^1,2, REN Jiahong^1,3, YE Jianren^1*, WANG Xiaoyue¹

Author information +

文章历史 +

摘要

吡咯伯克霍尔德氏菌JK-SH007是分离自杨树中的一种细菌,其对杨树病原真菌具有抑制作用,是一种具有生防应用潜力的有益菌株。为深入了解该菌株的发酵进程,放大发酵规模,并对发酵过程进行最优化控制,笔者对JK-SH007菌株液体分批发酵产生抑菌活性的动力学进行了研究。根据发酵实验数据,分别选用Logistic 方程、Luedeking-Piret 方程和Luedeking-Piret like方程建立了模拟菌体生长、抑制真菌活性产生和葡萄糖消耗的动力学模型。经拟合分析发现,动力学模型的计算值与实验值拟合良好,可直观反映JK-SH007菌株分批发酵过程中的动力学特征。

Abstract

Burkholderia pyrrocinia JK-SH007 isolated from poplar can inhibit the pathogenic fungi in poplar. To optimum fermentation process of strain JK-SH007, fermentation kinetics were investigated in this study. A kinetic model for the simulation of bacterial growth, the inhibition of fungal activity generation and glucose consumption were constructed by using Logistic equation, Luedeking-Piret equation and Luedeking-Piret like equation, respectively, based on the data from fermentation experiment. The calculated values in kinetic models fit well with experimental values which directly reflected the kinetic characteristics during the batch fermentation process of strain JK-SH007. Moreover, the fermentation kinetics model provided a foundation for the optimization and amplification of fermentation process.

引用本文

EndNote

Ris (Procite)

Bibtex

导出引用

李浩,任嘉红1,3,叶建仁,王小月. 吡咯伯克霍尔德氏菌JK-SH007分批发酵动力学模型的构建[J]. 南京林业大学学报（自然科学版）. 2016, 40(02): 173-178 https://doi.org/10.3969/j.issn.1000-2006.2016.02.029

LI Hao, REN Jiahong, YE Jianren, WANG Xiaoyue. Construction of batch fermentation kinetics model of Burkholderia pyrrocinia strain JK-SH007[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2016, 40(02): 173-178 https://doi.org/10.3969/j.issn.1000-2006.2016.02.029

中图分类号： Q939.95

参考文献

[1] 叶建仁, 任嘉红, 李浩, 等. 洋葱伯克霍尔德氏菌及其在林木病害防治中的应用[J]. 南京林业大学学报(自然科学版),2013, 37(4): 149-155.Doi:10.3969/j.issn.1000-2006.2013.04.028. Ye J R, Ren J H, Li H, et al. Application and its prospect analysis for Burkholderia cepacia in forest disease control[J]. Journal of Nanjing Forestry University(Natural Sciences Edition), 2013, 37(4):149-155.
[2] 查传勇, 董法宝, 杨悦, 等. 洋葱伯克霍尔德氏菌Lu10-1产生抗菌活性物质的发酵培养基和发酵条件优化[J]. 蚕业科学, 2009, 35(2): 229-235. Zha C Y, Dong F B, Yang Y, et al. Optimization of medium components and cultural conditions for producing antibacterial substances from Burkholderia cepacia Lu10 -1[J]. Science of Sericulture, 2009, 35(2):229-235.
[3] 权春善, 郑维, 曹治明, 等. 洋葱伯克霍尔德菌CF-66抗菌物质的分离纯化及性质的研究[J]. 微生物学报, 2005, 45(5): 707-710. Quan C S, Zeng W, Cao Z M,et al. Purification and properties of anitibiotic from Burkholderia cepacia CF-66[J]. Acta Microbiologica Sinica, 2005, 45(5):707-710.
[4] 刘俏, 权春善, 曾勇峰, 等. 洋葱伯克霍尔德菌CF-66发酵动力学研究[J]. 计算机与应用化学, 2008, 25(3): 353-356. Liu Q, Quan C S, Zeng Y F, et al. Study on the fermentation kinetics of Burkholderia cepacia CF-66[J]. Computers and Applied Chemistry, 2008, 25(3):353-356.
[5] 于乃功, 阮晓钢. 青霉素发酵过程优化控制问题及方法研究[J]. 国外医药(抗生素分册), 2004,25(3): 97-100. Yu N G, Ruan X G. Problems and methods study of penicillin fermentation process optimization[J]. World Notes on Antibiotics, 2004,25(3):97-100.
[6] 王普, 张亚庭, 王进秋, 等. 链霉素发酵控制[J]. 北京工业大学学报, 1993, 19(3): 115-118. Wang P, Zhang Y T, Wang J Q, et al. Streplomycin ferment process control[J]. Journal of Beijing Polytechnic University, 1993, 19(3):115-118.
[7] 刘峰, 杨桂英. 改变庆大霉素发酵工艺对其组分及效价的影响[J]. 福建师范大学学报(自然科学版), 2003, 19(3): 70-73. Liu F, Yang G Y. The improved fermentation techniques' positive influence on the components and potency of gentamicin broth[J]. Journal of Fujian Normal University(Nutaral Science), 2003, 19(3):70-73.
[8] 白亚磊, 徐庆阳, 谢希贤, 等. 溶氧控制对黄色短杆菌YILW合成L-异亮氨酸的影响[J]. 天津科技大学学报, 2011, 26(1): 5-9,30. Bai Y L, Xu Q Y, Xie X X, et al. Effect of dissolved oxygen control on L-isoleucine synthesis by Brevibacterium flavum YILW[J]. Journal of Tianjin University of Science & Technology, 2011, 26(1):5-9,30.
[9] 刘红, 蔡绍皙, 潘红春. 溶氧对L-天冬酰胺酶发酵的影响及其控制[J]. 中国生物制品学杂志, 2003, 16(6): 345-347. Liu H, Cai S X, Pan H C. Influence of dissloved oxygen on fermentation of L-asparaginase and control[J]. Chin J Biologicals, 2003, 16(6):345-347.
[10] 邓坤, 冀志霞, 陈守文. 溶氧对地衣芽孢杆菌DW2合成杆菌肽的影响[J]. 中国抗生素杂志, 2009, 34(11): 664-668. Deng K, Ji Z X, Chen S W. Effect of dissolved oxygen on bacitracin fermentation by Bacillus licheni form is DW2[J]. Chinese Journal of Antibiotics, 2009, 34(11):664-668.
[11] 刘冬, 李世敏, 许柏球, 等. pH值及溶解氧对灵芝多糖深层液态发酵的影响与控制[J]. 食品与发酵工业, 2001,27(6): 7-10. Liu D, Li S M, Xu B Q, et al. Studies on effects of pH value and dissolved oxygen on Ganoderma polysaccharides production by submerged fermentation and control measures for process[J]. Food and Fermentation Industries, 2001,27(6):7-10.
[12] 孟慧娟. pH影响食品废弃物和剩余污泥联合发酵产酸及发酵液用于生物合成聚羟基烷酯的研究[D]. 杭州: 浙江大学, 2012. Meng H J. The effect of pH on the fermentation for VFAs with food waste and excess sludge and the biosynthesis of polyhydroxyalkanoate with fermentation liquid[D].Hangzhou:Zhejiang University, 2012.
[13] Ren J H, Ye J R, Liu H, et al. Isolation and characterization of a new Burkholderia pyrrocinia strain JK-SH007 as a potential biocontrol agent[J]. World Journal of Microbiology and Biotechnology, 2011, 27(9): 2203-2215.
[14] 李浩, 任嘉红, 叶建仁. 响应面法优化生防菌吡咯伯克霍尔德氏菌JK-SH007的发酵工艺[J]. 生物工程学报, 2013, 29(2): 243-246. Li H, Ren J H, Ye J R. Optimization of biocontrol agent Burkholderia pyrrocinia strain JK-SH007 fermentation by response surface methodology[J]. Chinese Journal of Biotechnology,2013,29(2):243-246.
[15] 任嘉红, 班虎栋, 叶建仁, 等. 吡咯伯克霍尔德氏菌 JK -SH007 的发酵条件及其对杨树溃疡病的防治效果[J]. 中国生物防治, 2010, 26(3): 300-306. Ren J H, Ban H D, Ye J R, et al. Fermentation conditions of antagonistic strain Burkholderia pyrrocinia JK -SH007 and its control effect on poplar canker disease[J]. Chinese Journal of Biological Control, 2010, 26(3):300-306.
[16] 葛亮, 房耀维, 吴文惠, 等. 海洋细菌 LP621 右旋糖苷酶分批发酵动力学研究[J].海洋科学, 2012, 36(7): 39-43. Ge L, Fang Y W, Wu W H, et al. Kinetics analysis for dextranase of Pseudoalteromonas tetraodonis LP621 by liquid fermentation[J]. Marine Science, 2012, 36(7):39-43.
[17] Elmer L, Gaden J R. Fermentation process kinetics[J]. Journal of Biochemical and Microbiological Technology and Engineering, 1959, 7(4):413-429.
[18] Luedeking R, Piret E L. A kinetic study of the lactic acid fermentation. Batch process at controlled pH[J]. Biochem Micro-biol Technol Eng, 1959,1(4):393-412.
[19] 王莹, 朴美子, 孙永海. 基于遗传算法的羊肚菌液体发酵动力学模型的建立[J]. 生物工程学报, 2008, 24(8): 1454-1457. Wang Y, Piao M Z, Sun Y H. Genetic algorithm for fermentation kinetics of submerged fermentation by Morchella[J]. Chin J Biotech, 2008, 24(8): 1454-1457.
[20] 宋文军, 陈宁, 王健, 等. L-色氨酸产生菌分批发酵动力学模型[J]. 无锡轻工大学学报, 2002, 21(4): 340-343,356. Song W J, Chen N, Wang J, et al. The kinetics of batch fermentation of L-Tryptophan-Producing strain[J]. Journal of Wuxi University of Light Industry, 2002, 21(4):340-343,356.

基金

收稿日期:2014-11-26 修回日期:2015-12-26
基金项目:国家林业公益性行业科研专项项目(201304404); 江苏高校优势学科建设工程资助项目(PAPD); 国家自然科学基金项目(31270684,31470645)
第一作者:李浩(lihaonfu@163.com)。*通信作者:叶建仁(jrye@njfu.edu.cn),教授。
引文格式:李浩,任嘉红,叶建仁,等. 吡咯伯克霍尔德氏菌JK-SH007分批发酵动力学模型的构建[J]. 南京林业大学学报(自然科学版),2016,40(2):173-178.