鹅掌楸树叶和树皮提取物的抑菌活性研究

赵亚琦; 吕言; 张文军; 魏继福; 陈金慧; 施季森; 成铁龙

doi:10.3969/j.issn.1000-2006.2016.02.013

鹅掌楸树叶和树皮提取物的抑菌活性研究

赵亚琦,吕言,张文军,魏继福,陈金慧,施季森,成铁龙

南京林业大学学报（自然科学版） ›› 2016, Vol. 40 ›› Issue (02) : 76-80.

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南京林业大学学报（自然科学版） ›› 2016, Vol. 40 ›› Issue (02) : 76-80. DOI: 10.3969/j.issn.1000-2006.2016.02.013

研究论文

鹅掌楸树叶和树皮提取物的抑菌活性研究

赵亚琦¹,吕言¹,张文军¹,魏继福²,陈金慧¹,施季森¹,成铁龙^1*

作者信息 +

Anti-bacterial effects of extracts from the Liriodendron L. barks and leaves

ZHAO Yaqi¹,LYU Yan¹,ZHANG Wenjun¹,WEI Jifu²,CHEN Jinhui¹,SHI Jisen¹,CHENG Tielong^1*

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摘要

林源生物活性物质的研究和利用是森林资源利用的重要方面。为研究重要的药源植物鹅掌楸属植物树皮及树叶提取物的抑菌活性,用琼脂稀释法检测鹅掌楸属不同种间和种内树皮及叶片提取物对大肠杆菌、绿脓杆菌、金黄色葡萄球菌和粪链球菌的抑菌反应。结果表明:鹅掌楸属3个树种的树皮和树叶提取物对大肠杆菌、绿脓杆菌、金黄色葡萄球菌和粪链球菌有明显的抑菌效果; 树皮提取物对大肠杆菌、绿脓杆菌、金黄色葡萄球菌及粪链球菌的最低抑菌质量浓度(MIC)分别为5.12、0.64、0.64 和0.64 mg/mL; 树叶提取物的MIC分别为40.96、2.56、2.56和2.56 mg/mL。鹅掌楸属树种不同部位的提取物抑菌效果有明显差别,树皮提取物抑菌效果强于树叶提取物,但不同种间提取物的抑菌效果差异不显著。

Abstract

Forest plant is one of the important medicine resources. Liriodendron L. is not only an important timber productive genus with superior wood quality, but also an important traditional Chinese medicine. Here, we report the results of anti-bacterial activities of extracts from Liriodendron L. barks and leaves. Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Streptococcus faecalis were test strains, and the anti-microbial activities of the extracts were determined by agar dilution methods. The results showed that the growth of Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Streptococcus faecalis on agar were obviously inhibited by adding extracts from Liriodendron L. barks and leaves. The MIC of the barks extracts were 5.12, 0.64, 0.64 and 0.64 mg/mL, respectively, and the MIC of the leave extracts were 40.96, 2.56, 2.56 and 2.56 mg/mL, respectively. The anti-microbial activities of the extracts from the barks were more effective than that of the leaves extracts. However, the anti-microbial activities among species within the Liriodendron L. were not significant statistically.

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赵亚琦,吕言,张文军,魏继福,陈金慧,施季森,成铁龙. 鹅掌楸树叶和树皮提取物的抑菌活性研究[J]. 南京林业大学学报（自然科学版）. 2016, 40(02): 76-80 https://doi.org/10.3969/j.issn.1000-2006.2016.02.013

ZHAO Yaqi,LYU Yan,ZHANG Wenjun,WEI Jifu,CHEN Jinhui,SHI Jisen,CHENG Tielong. Anti-bacterial effects of extracts from the Liriodendron L. barks and leaves[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2016, 40(02): 76-80 https://doi.org/10.3969/j.issn.1000-2006.2016.02.013

中图分类号： TQ468

参考文献

[1] 王章荣. 鹅掌楸属(Liriodendron)杂交育种回顾与展望[J]. 南京林业大学学报(自然科学版),2003,27(3):76-78. Doi:10.3969/j.issn.1000-2006.2003.03.019. Wang Z R. The review and outlook on hybridization in tulip tree breeding in China [J]. Journal of Nanjing Forestry University(Natural Sciences Edition),2003,27(3):76-78.
[2] 南京林产工业学院林学系育种组. 亚美杂种马褂木的育成[J]. 林业科技通讯,1973(12):10-11. Nanjing Technological College of Forest Products. The breeding of Liriodendron hybrids(L. chinense ×L. tulipifera)[J].Forest Science and Technology,1973(12):10-11.
[3] 赵守训. 中药辞海:第3卷[M].北京:中国医药科技出版社,1997:949-950. Zhao S X. The encyclopedia of traditional Chinese medicine(Vol. III )[M]. Beijing:China Press of Traditional Chinese Medical Science and Technology,1997:949-950.
[4] Chiu C C, Chou H L, Wu P F, et al. Bio-functional constituents from the stems of Liriodendron tulipifera[J]. Molecules(Basel, Switzerland), 2012, 17(4): 4357-4372.Doi:10.3390/molecules17044357.
[5] Graziose R, Rathinasabapathy T, Lategan C, et al. Antiplasmodial activity of aporphine alkaloids and sesquiterpene lactones from Liriodendron tulipifera L[J]. Journal of Ethnophar Macology, 2011, 133(1): 26-30.Doi:10.1016/j.jep.2010.08.059.
[6] Yang D T, Lin S S, Chen J H, et al.(+)-and(-)-liriodenol, a pair of novel enantiomeric lignans from Liriodendron hybrid[J]. Bioorganic & Medicinal Chemistry Letters, 2015, 25(9): 1976-1978. Doi:10.1016/j.bmcl.2015.03.015.
[7] Rahman M M, Lopa S S, Sadik G, et al. Antibacterial and cytotoxic compounds from the bark of Cananga odorata[J]. Fitoterapia, 2005, 76(7-8): 758-761.Doi:10.1016/j.fitote.2005.08.011.
[8] Hufford C D,Funderburk M J,Morgan J M, et al. Two antimicrobial alkaloids from heartwood of Liriodendron tulipifera L. [J]. Journal of Pharmaceutical Sciences,1975,64(5):789-792.Doi: 10.1002/jps.2600640512.
[9] 周坚,樊汝汶. 中国鹅掌楸传粉生物学研究[J]. 植物学通报,1999,16(1):75-79.Doi:10.3969/j.issn.1674-3466.1999.01.011. Zhou J,Fan R W. Pollination of Liriodendron chinense [J]. Chinese Bulletin of Botany,1999,16(1):75-79.
[10] 陈金慧,施季森,诸葛强,等. 杂交鹅掌楸体细胞胚胎发生研究[J].林业科学,2003,39(4):49-53.Doi:10.3321/j.issn:1001-7488.2003.04.008. Chen J H, Shi J S, Zhuge Q, et al. Studies on the somatic embryogenesis of Liriodendron hybrids(L.chinense×L.tulipifera)[J].Scientia Silvae Sinicae,2003,39(4):49-53.
[11] 李火根,施季森. 杂交鹅掌楸良种选育与种苗繁育[J].林业科技开发,2009,23(3):1-5.Doi:10.3969/j.issn.1000-8101.2009.03.001. Li H G,Shi J S. The Selective improved variety and seedling breeding of Liriodendron hybrids(L.chinense×L.tulipifera)[J]. China Forestry Science and Technology,2009,23(3):1-5.
[12] 李书华,陈封政,李仲芳,等. 鹅掌楸树皮提取物的抑菌作用[J].江苏农业科学,2008,36(3):114-115.Doi:10.3969/j.issn.1002-1302.2008.03.040. Li S H,Chen F Z,Li Z F, et al. Bacteriostatic activity of extracts from barks of Liriodendron chinense [J]. Jiangsu Agricultural Sciences,2008,36(3):114-115.
[13] 曾超珍,刘志祥. 鹅掌楸抑菌物质的体外抗菌活性及稳定性试验[J].湖北农业科学,2009,48(5):1222-1224.Doi:10.3969/j.issn.0439-8114.2009.05.063. Zheng C Z,Liu Z Y. Bacteriostatic activity and stability of extracts from Liriodendron chinense [J]. Hubei Agricultural Sciences,2009,48(5):1222-1224.
[14] 陈金慧,施季森,贾爱群,等. 一种鹅掌楸的多酚类提取物及其提取方法和应用:CN102335233A [P].2012-02-01. Chen J H,Shi J S,Jia A Q, et al. Polyphenol extract of Liriodendron chinense or Liriodendron tulipifera, and extraction method and applications:CN102335233A [P]. 2012-02-01.
[15] 施季森,陈金慧,贾爱群,等. 一种鹅掌楸的生物碱提取物及其提取方法和应用:CN102335234A [P]. 2012-02-01. Shi J S,Chen J H,Jia A Q, et al. Alkaloid extract of Liriodendron chinense or Liriodendron tulipifera, and extraction method and applications:CN102335234A [P]. 2012-02-01.
[16] Chen J H,Yang G X,Ding Q,et al. In vitro tumor cytotoxic activities of extracts from three Liriodendron plants [J]. Pakistan Journal of Pharmaceutical Sciences,2013,26(2):233-237.
[17] 李晓娜,朱文清, 陈展慧,等. 三种木兰科植物茎和叶提取物的抑菌活性研究[J]. 南京大学学报(自然科学),2010,46(3):197-202. Li X N,Zhu W Q,Chen Z H, et al. Antimicrobial activity of stem and leaf extracts from three species of the Magnoliaceae [J]. Journal of Nanjing University(Natural Sciences),2010,46(3):197-202.
[18] Makarasen A,Sirithana W, Mogkhuntod S, et al. Cytotoxic and antimicrobial activities of aporphine alkaloids isolated from Stephania venosa(Blume)Spreng [J]. Planta Medica,2011,77(13):1519-1524.Doi:10.1055/s-0030-1270743.
[19] Omar H,Hashim N M,Zajmi A, et al. Aporphine alkaloids from the leaves of Phoebe grandis (Nees)Mer.(Lauraceae)and their cytotoxic and antibacterial activities [J]. Molecules,2013,18(8):8994-9009.Doi:10.3390/molecules18088994.
[20] 李敏,向平,杨志伟,等. 杨梅不同部位单宁含量与结构研究[J]. 林产化学与工业,2008,28(3):55-60. Doi: 10.3321/j.issn:0253-2417.2008.03.011. Li M,Xiang P,Yang Z W, et al. Content and structure of tannins in different parts of Bayberry(Myrica rubra(Lour.)S. et Zucc.)[J]. Chemistry and Industry of Forest Products,2008,28(3):55-60.
[21] 张艳军,彭重威,钟秋平,等. 广西红树植物银叶树不同部位提取物体外抗氧化性分析[J]. 南方农业学报,2013,44(12):2066-2070. Doi:10.3969/j:issn.2095-1191.2013.12.2066. Zhang Y J,Peng C W,Zhong Q P, et al. In vitro antioxidant activities in different parts of Heritiera littoralis dry and of mangrove plants in Guangxi [J]. Journal of Southern Agriculture,2013,44(12):2066-2070.

基金

收稿日期:2015-04-28 修回日期:2015-07-23
基金项目:教育部新世纪优秀人才支持计划; 国家林业局“948”引进项目(2012-4-07); 江苏高校优势学科建设工程资助项目(PAPD)
第一作者:赵亚琦(zyq_zhaoqi_2006@163.com)。*通信作者:成铁龙(ctielong@njfu.edu.cn),副研究员。
引文格式:赵亚琦,吕言,张文军,等. 鹅掌楸树叶和树皮提取物的抑菌活性研究[J]. 南京林业大学学报(自然科学版),2016,40(2):76-80.