薄壳山核桃嫁接愈合过程中差异蛋白质的分析

莫正海; 何海洋; 陈文静; 邓秋菊; 彭方仁

doi:10.3969/j.issn.1000-2006.201703021

莫正海,何海洋,陈文静,邓秋菊,彭方仁

南京林业大学学报（自然科学版） ›› 2017, Vol. 41 ›› Issue (06) : 19-25.

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南京林业大学学报（自然科学版） ›› 2017, Vol. 41 ›› Issue (06) : 19-25. DOI: 10.3969/j.issn.1000-2006.201703021

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薄壳山核桃嫁接愈合过程中差异蛋白质的分析

莫正海,何海洋,陈文静,邓秋菊,彭方仁^*

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Analysis of the differential proteins in the graft healing process of pecan(Carya illinoinensis)

MO Zhenghai, HE Haiyang, CHEN Wenjing, DENG Qiuju, PENG Fangren^*

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摘要

【目的】嫁接是繁殖薄壳山核桃的重要手段,拟从蛋白质水平揭示薄壳山核桃嫁接愈合的机理。【方法】利用双向电泳技术结合MALDI-TOF/TOF-MS,研究了薄壳山核桃嫁接愈合部位4个发育时期(嫁接后第1、6、10和25天)的差异蛋白。【结果】共成功鉴定48个差异蛋白,这些差异蛋白按功能可分为7类,包括能量代谢、抗性及防御、细胞生长、次生代谢、蛋白质合成、氨基酸代谢以及未知功能蛋白。【结论】果糖二磷酸醛缩酶、磷酸甘油酸激酶、丙酮酸脱羧酶、三磷酸腺苷酶能够为嫁接愈合提供所需的能量; 抗坏血酸过氧化物酶、过氧化物还原酶能够有效清除嫁接愈合过程中积累过多的活性氧; 可溶性无机焦磷酸酶可以促进愈伤组织的增殖; 类胱天冬蛋白酶、α微管蛋白可能参与管状分子的分化。

Abstract

【Objective】Grafting is an important method for propagating pecan. This paper aimed to reveal the mechanism underlying graft healing on the proteomic level. 【Method】 We investigated the differential proteins in the graft union of pecans at four developmental stages(1, 6, 10 and 25 days after grafting)based on two-dimensional gel electrophoresis and MALDI-TOF/TOF-MS. 【Result】 In total, 48 differential proteins were identified, and these identified proteins were classified into seven functional categories: energy metabolism, stress and defense responses, cell growth, secondary metabolism, protein synthesis, amino acid metabolism and function unknown. 【Conclusion】Fructose-bisphosphate aldolase, phosphoglycerate kinase, pyruvate decarboxylase and adenosine triphosphatase could provide adequate energy for the graft healing of pecan. Ascorbate peroxidase and peroxiredoxins can eliminate the excessive reactive oxygen species caused by grafting. Soluble inorganic pyrophosphatase may play a role in promoting callus proliferation. Metacaspase and alpha tubulin may be associated with tracheary element differentiation.

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莫正海,何海洋,陈文静,邓秋菊,彭方仁. 薄壳山核桃嫁接愈合过程中差异蛋白质的分析[J]. 南京林业大学学报（自然科学版）. 2017, 41(06): 19-25 https://doi.org/10.3969/j.issn.1000-2006.201703021

MO Zhenghai, HE Haiyang, CHEN Wenjing, DENG Qiuju, PENG Fangren. Analysis of the differential proteins in the graft healing process of pecan(Carya illinoinensis)[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2017, 41(06): 19-25 https://doi.org/10.3969/j.issn.1000-2006.201703021

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