平安竹纤维形态变异特征及其成因分析

魏强; 高志鹏; 郭琳; 胡佩; 夏苏娟; 丁雨龙

doi:10.3969/j.issn.1000-2006.201712010

平安竹纤维形态变异特征及其成因分析

魏强,高志鹏,郭琳,胡佩,夏苏娟,丁雨龙

南京林业大学学报（自然科学版） ›› 2018, Vol. 42 ›› Issue (04) : 25-31.

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南京林业大学学报（自然科学版） ›› 2018, Vol. 42 ›› Issue (04) : 25-31. DOI: 10.3969/j.issn.1000-2006.201712010

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平安竹纤维形态变异特征及其成因分析

魏强, 高志鹏,郭琳, 胡佩, 夏苏娟, 丁雨龙

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Characterization of fiber morphological variation of Pseudosasa japonica var. tsutsumiana and the underlying mechanism

WEI Qiang,GAO Zhipeng, GUO Lin,HU Pei, XIA Sujuan, DING Yulong

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摘要

【目的】阐明矢竹矮秆稳定变异体——平安竹纤维形态学特征并初步阐释其变异机制。【方法】利用石蜡切片及数量统计学方法进行显微观测与数据分析; qRT-PCR分析基因相对表达量; UPLC/GC-MS法测定赤霉素含量。【结果】平安竹竹秆不同部位纤维长度、长宽比及壁腔比显著小于矢竹; 但其宽度与腔径值显著大于矢竹; 而其不同部位纤维壁厚值虽小于矢竹,但差异不显著。平安竹地下茎纤维在长度、长宽比、壁厚及壁腔比上显著小于矢竹,但其宽度与腔径值则显著大于矢竹。形态解剖学分析显示,平安竹地下茎纤维细胞形态变异出现于节间活跃生长阶段。赤霉素及细胞壁与细胞骨架相关基因表达量分析结果表明,赤霉素信号途径在平安竹节间生长起始阶段即受到抑制; 而其细胞壁与细胞骨架相关下游功能基因则在其伸长早期与活跃阶段显著低于矢竹; 赤霉素含量分析也证实各赤霉素含量早在平安竹节间伸长起始阶段就低于矢竹。【结论】赤霉素信号途径受抑导致其下游细胞壁与细胞骨架组织相关基因下调是最终导致平安竹纤维细胞生长异常的原因之一。

Abstract

Abstract: 【Objective】The study aimed to elucidate the fiber morphological characteristics, as well as the underlying mechanisms, in Pseudosasa japonica var. tsutsumiana, a stable dwarf variant of P. japonica. 【Method】 Microscope observation and paraffin sections were used to compare cell morphology. Relative transcript abundance was measured by quantitative reverse transcription polymerase chain reaction(qRT-PCR), and ultra performance liquid chromatography/gas chromatography-mass spectrometry(UPLC/GC-MS)was used to investigate gibberellin(GA)abundance. 【Result】 Values for fiber length, the ratio of length to width, and the runkel ratio were significantly smaller in P. japonica var. tsutsumiana culm than in P. japonica. However, the width and lumen diameter values of the variant fiber were larger than that in the wild type. Although the fiber cell wall thickness in different culm parts of P. japonica var. tsutsumiana was smaller than those in P. japonica, the difference was statistically insignificant. In addition, the fiber length, length to width ratio, cell wall thickness, and runkel ratio in P. japonica var. tsutsumiana rhizomes were also significantly smaller than those in P. japonica. Similar to the culm, the values for width and lumen diameter in the rhizome fibers of the variant were larger than those of the wild type. Anatomical analysis revealed that irregular phenotypes of fiber cells in P. japonica var. tsutsumiana clearly emerged at the initiation stage of internode elongation. Relative gene expression level measurements of the GA, cell wall growth and cytoskeleton-related genes on the internodes at different elongation stages showed that the GA pathway in P. japonica var. tsutsumiana was inhibited at the early stage of internode growth, and that genes relating to cell wall and cytoskeleton organization were significantly downregulated on the internode during the active growth stage of the variant. Compared with the wild type, the GA content was indeed lower at the internode in the early elongation stage of P. japonica var. tsutsumiana, as was further demonstrated by the qRT-PCR results. 【Conclusion】The inhibition of the GA signal pathway, which results in the downregulation of its downstream genes, such as those related to cell wall growth and cytoskeleton organization, is one of the factors that causes abnormal growth of fiber cells in P. japonica var. tsutsumiana.

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魏强,高志鹏,郭琳,胡佩,夏苏娟,丁雨龙. 平安竹纤维形态变异特征及其成因分析[J]. 南京林业大学学报（自然科学版）. 2018, 42(04): 25-31 https://doi.org/10.3969/j.issn.1000-2006.201712010

WEI Qiang,GAO Zhipeng, GUO Lin,HU Pei, XIA Sujuan, DING Yulong. Characterization of fiber morphological variation of Pseudosasa japonica var. tsutsumiana and the underlying mechanism[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2018, 42(04): 25-31 https://doi.org/10.3969/j.issn.1000-2006.201712010

中图分类号： S718 S795

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