镉污染土壤中添加外源有机物料对杨树苗期生长的影响

曹莉; 王艮梅; 续卫利; 普慧梅; 杨园

doi:10.3969/j.issn.1000-2006.201801026

曹莉,王艮梅,续卫利,普慧梅,杨园

南京林业大学学报（自然科学版） ›› 2018, Vol. 42 ›› Issue (04) : 68-74.

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南京林业大学学报（自然科学版） ›› 2018, Vol. 42 ›› Issue (04) : 68-74. DOI: 10.3969/j.issn.1000-2006.201801026

研究论文

镉污染土壤中添加外源有机物料对杨树苗期生长的影响

曹莉^1,3,王艮梅^1,2*,续卫利⁴,普慧梅^1,2,杨园^1,3

作者信息 +

Effects of application of poplar litter and sludge compost on poplar seedling growth in Cd²⁺-contaminated soil

CAO Li^1,3, WANG Genmei^1,2*, XU Weili⁴,PU Huimei^1,2, YANG Yuan^1,3

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摘要

【目的】探究两种常见的有机物料(杨树凋落物和城市生活污泥堆肥)进入镉(Cd)污染土壤后对该土壤上杨树的生长及生理生化产生的影响。【方法】通过土壤盆栽试验,研究添加有机物料对不同含量Cd²⁺污染土壤上‘南林895'杨(Populus&#215;euramericana ‘Nanlin 895')生长3个月后植株地上部分生物量、叶片中光合色素含量、抗氧化保护酶活性以及丙二醛(MDA)含量的影响。【结果】①添加污泥堆肥(CS)处理的杨树地上部分干质量、叶片中光合色素含量均明显高于同等条件下的对照(CK)处理; 添加杨树叶(PL)处理的杨树地上部分干质量在低Cd²⁺(0、10 mg/kg)污染土壤上高于对照处理,在高Cd²⁺(50、100、150 mg/kg)污染土壤上则显著低于对照处理; 而与对照相比,除了不添加Cd²⁺外,PL处理下叶片中光合色素含量都低于CK处理。②随着Cd²⁺含量升高,3种处理杨树叶片SOD与CAT活性均呈现先上升后下降的趋势,CS处理杨树叶片SOD及CAT的活性均明显高于同等条件下的对照(CK)处理; PL处理叶片中SOD及CAT的活性在不添加外源Cd²⁺时均高于CK处理,而Cd²⁺含量≥50 mg/kg时均低于CK处理。此外,杨树叶片MDA含量受外源物料类型的影响,CS处理的MDA含量均低于同等条件下的CK处理; PL处理的MDA含量则高于同等条件下CK处理的。【结论】镉污染土壤上不同处理间杨树生长的状况总体表现为:添加污泥堆肥处理的杨树生长最好,其次是对照处理,而杨对凋落物处理的相对最差。

Abstract

Abstract: 【Objective】This study aimed to explore the effects of application of two types of common organic material(poplar litter and sludge compost)on physiological and biochemical indexes of poplar grown in Cd²⁺-contaminated soil.【Method】A greenhouse pot experiment was conducted to study aerial biomass, photosynthetic pigment, and antioxidant enzyme activities of a hybrid poplar(Populus&#215;euramericana ‘Nanlin 895')growing for three months in Cd²⁺-contaminated soil with organic materials. A gradient test design with five Cd²⁺ concentrations(0, 10, 50, 100 and 150 mg/kg)and two organic amendments [polar litter(PL)and sludge compost(CS)] with three replicates was established. 【Result】① The dry weight of the aerial parts and the contents of photosynthetic pigments in the CS treatment were significantly higher than those in the CK(without organic materials)with the same Cd²⁺ concentration; the dry weights of the aerial parts of poplar in the PL treatment were higher than those in CK when the Cd²⁺ concentration was no more than 10 mg/kg. However, when the concentration of Cd²⁺ was higher than or equal to 50 mg/kg, the dry weight in the PL treatment was significantly lower than that in the CK. Compared with the CK, the photosynthetic pigment content were all lower in the PL treatment(except for the treatment with no Cd²⁺ addition). ② With increase in Cd²⁺ concentration, the activities of SOD(superoxide dismutase)and CAT(catalase)were both increased at first and then decreased for the three treatments. In the CS treatment, the activities of SOD and CAT were significantly higher than those in the CK. With no Cd²⁺ addition, the activities of SOD and CAT in the PL treatment were higher than those in the CK. However, with Cd²⁺ addition, the activities of SOD and CAT were lower in the PL treatment than those in the CK treatment when the concentration of Cd²⁺ was higher than 50 mg/kg. In addition, the MDA(malondialdehyde)content in poplar leaves was also influenced by the type of organic material added. The addition of compost sludge decreased the MDA content in leaves of poplar for all experimental Cd²⁺ concentrations, while the addition of poplar litter increased the MDA content in poplar leaves.【Conclusion】In general, the growth of poplar in Cd²⁺-contaminated soil was better in the CS treatment than in the CK, but in the PL treatment, the growth of poplar was poorer than in either of the other two treatments.

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曹莉,王艮梅,续卫利,普慧梅,杨园. 镉污染土壤中添加外源有机物料对杨树苗期生长的影响[J]. 南京林业大学学报（自然科学版）. 2018, 42(04): 68-74 https://doi.org/10.3969/j.issn.1000-2006.201801026

CAO Li, WANG Genmei, XU Weili,PU Huimei, YANG Yuan. Effects of application of poplar litter and sludge compost on poplar seedling growth in Cd²⁺-contaminated soil[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2018, 42(04): 68-74 https://doi.org/10.3969/j.issn.1000-2006.201801026

中图分类号： S714.6 X53

参考文献

[1] LAUREYSENS I, BLUST R, TEMMERMAN L D, et al. Clonal variation in heavy metal accumulation and biomass production in a poplar coppice culture: I. Seasonal variation in leaf, wood and bark concentrations[J].Environmental Pollution, 2004,131(3):485-494. DOI: 10.1016/j.envpol.2004.02.009.
[2] GIACHETTI G, SEBASTIANI L.Metal accumulation in poplar plant grown with industrial wastes[J].Chemosphere, 2006, 64(3):446-454. DOI: 10.1016/j.chemosphere.2005.11.021.
[3] DURAND T C, SERGEANT K, PLANCHON S, et al. Acute metal stress in Populus tremula &#215; Palba(717-1B4 genotype): leaf and cambial proteome changes induced by cadmium²⁺[J].Proteomics, 2010, 10(3):349-368. DOI:10.1002/pmic.200900484.
[4] IORI V, GAUDET M, FABBRINI F, et al. Physiology and genetic architecture of traits associated with cadmium tolerance and accumulation in Populus nigra L.[J].Trees, 2016, 30(1):125-139. DOI:10.1007/s00468-015-1281-5.
[5] 张东为, 崔建国, 戈素芬, 等. 土壤镉污染对不同品种杨树生长状况的影响[J].水土保持通报, 2008, 28(3):59-64. DOI: 10.13961/j.cnki.stbctb. 2008.03.035. ZHANG D W, CUI J G, GE S F, et al. Effect of Cd contamination in soil on growth of poplar of different varieties[J].Bulletin of Soil and Water Conservation, 2008, 28(3):59-64.
[6] 张春燕, 王瑞刚, 范稚莲, 等. 杨树和柳树富集的品种差异性[J].农业环境科学学报, 2013, 32(3):530-538. DOI:10.11654/jaes.2013.03.019. ZHANG C Y, WANG R G, FAN Z L, et al. Difference in cadmium, zinc and lead accumulation of poplar and willow species[J].Journal of Agro-Environmental Science, 2013, 32(3):530-538.
[7] WU F, YANG W, ZHANG J, et al. Cadmium accumulation and growth responses of a poplar(Populus deltoides &#215; Populus nigra)in cadmium contaminated purple soil and alluvial soil[J]. Journal of Hazardous Materials, 2010, 177(1):268-273. DOI: 10.1016/j.jhazmat.2009.12.028.
[8] 宋蒙亚, 李忠佩, 刘明, 等. 不同有机物料组合对土壤养分和生化性状的影响[J].中国农业科学, 2013, 46(17):3594-3603. DOI:10.3864/j.issn. 0578-1752.2013.17.008. SONG M Y, LI Z P, LIU M, et al. Effects of mixtures of different organic materials on soil nutrient content and soil biochemical characteristics[J]. Scientia Agricultura Sinica, 2013, 46(17):3594-3603.
[9] 朱青藤, 申连玉, 钱黎慧, 等. 有机物料对白土土壤胡敏酸结构特征的影响[J].植物营养与肥料学报, 2015, 21(2):396-403. DOI:10.11674/ zwyf. 2015.0214. ZHU Q T,SHEN L Y,QIAN L H, et al. Effects of organic material application on the structures of humic acids in low yield paddy soil[J]. Journal of Plant Nutrition and Fertilizer, 2015, 21(2):396-403.
[10] 姚桂华, 徐海舟, 朱林刚, 等. 不同有机物料对东南景天修复重金属污染土壤效率的影响[J].环境科学, 2015, 36(11):4268-4276. DOI:10.13227/ j.hjkx.2015.11.043. YAO G H, XU H Z, ZHU L G, et al. Effects of different kinds of organic materials on soil heavy metal phytoremediation efficiency by Sedum alfredii Hance[J].Environmental Science, 2015, 36(11):4268-4276.
[11] 詹绍军, 喻华, 冯文强, 等.有机物料与石灰对土壤和镉有效性的影响[J].西南农业学报, 2011, 24(3):999-1003. DOI:10.3969/ j.issn.1001-4829. 2011.03.034. ZHAN S J, YU H, FENG W Q, et al. Effects of different organic material and lime on soil pH and available cadmium[J].Southwest China Journal of Agricultural Sciences, 2011, 24(3):999-1003.
[12] NARWAL R P, SINGH B R. Effect of organic materials on partitioning, extractability and plant uptake of metals in alum shale soil [J].Water, Air, & Soil Pollution, 1998, 103:405-421. DOI:10.1023/a:1004912724284.
[13] 王艮梅. 农田土壤中水溶性有机物的动态及其对重金属铜、镉环境行为的影响[D].南京:南京农业大学,2004. WANG G M. Dynamics of dissolved organic matter and its impact on the behaviors of copper and cadmium in farmland[D].Nanjing: Nanjing Agricultural University,2004.
[14] 王林, 秦旭, 徐应明,等. 污灌区镉污染菜地的植物阻隔和钝化修复研究[J].农业环境科学学报,2014, 33(11):2111-2117. DOI:10.11654/jaes. 2014.11.006. WANG L, QIN X, XU Y M, et al.Phytoexclusion and in-situ immobilization of cadmium in vegetable field in sewage irrigation region[J]. Journal of Agro-Environment Science, 2014, 33(11):2111-2117.
[15] 王逊. 堆肥污泥对林地土壤及植物的影响研究[D]. 南京:南京林业大学, 2011. WANG X. Effects of sewage sludge compost on soil-plant systems [D]. Nanjing: Nanjing Forestry University, 2011.
[16] 陈桂梅, 刘善江, 田野,等. 污泥堆肥的应用研究进展[J]. 广州化工, 2010, 38(12): 15-17. DOI:10.3969/j.issn.1001-9677.2010.12.005. CHEN G M, LIU S J, TIAN Y, et al. Application and development of sewage sludge compost in agriculture[J].Guangzhou Chemical Industry, 2010, 38(12): 15-17.
[17] 鲍士旦. 土壤农化分析[M]. 3版. 北京:中国农业出版社, 2008: 30-48,76-78.
[18] 中国科学院南京土壤研究所. 土壤理化分析[M]. 上海:上海科学技术出版社, 1978:45-62.
[19] 李志丹, 韩瑞宏, 廖桂兰, 等. 植物叶片中叶绿素提取方法的比较研究[J].广东第二师范学院学报, 2011, 31(3):80-83. DOI:10.3969/j.issn. 1007-8754.2011.03.015. LI Z D, HAN R H, LIAO G L, et al. A comparative study on the different extraction techniques about the chlorophyll concentration of plant leaf[J].Journal of Guangdong University of Education, 2011, 31(3):80-83.
[20] 李玲.植物生理学模块实验指导[M]. 北京:科学出版社, 2009: 80-81, 97-98.
[21] JAKOVLJEVIC'T, BUBALO M C, ORLOVIC'S, et al. Adaptive response of poplar(Populus nigra L.)after prolonged Cd exposure period[J].Environmental Science and Pollution Research, 2014, 21(5):3792-3802. DOI: 10.1007/s11356-013-2292-7.
[22] 张帆, 万雪琴, 翟晶. 镉处理下增施氮对杨树叶绿素合成和叶绿体超微结构的影响[J].核农学报,2014,28(3):485-491.DOI: 10.11869/j. issn.100-8551. 2014.03.0485 ZHANG F, WAN X Q, ZHAI J. Effects of nitrogen supplement on chlorophyll synthesis and chloroplast ultrastructure of poplar plants under cadmium stress[J]. Journal of Nuclear Agricultural Sciences, 2014, 28(3):485-491.
[23] 冯世静, 杨途熙, 张艳军,等. 镉胁迫对杨树光合特性的影响[J].农业环境科学学报, 2013,32(3):539-547. DOI:10.11654/jaes.2013.03.020. FENG S J, YANG T X, ZHANG Y J, et al. Effects of cadmium on photosynthetic gas exchange and chlorophyll fluorescence of two species of poplar[J].Journal of Agro-Environment Science,2013, 32(3):539-547.
[24] DRAZKIEWICZ M, BASZYNSKI T. Growth parameters and photosynthetic pigments in leaf segments of Zea mays exposed to cadmium, as related to protection mechanisms [J]. Journal of Plant Physiology, 2005, 162(9): 1013-1021. DOI:10.1016/j.jplph.2004.10.010.
[25] ZHANG X A, WANG Z H, ZHANG X Q, et al. Effects of heavy metals and saline-alkali on growth, physiology and biochemistry of Orychophragmus violaceus[J]. Agricultural Science & Technology, 2012, 13(7):1478-1483. DOI:10.3969/j.issn.1009-4229-B.2012.07.021.
[26] 潘逸, 周立祥. 小麦地土壤水溶性有机物动态及对土壤铜镉活性的影响:田间微区试验[J].环境科学, 2007, 28(4): 4859-4865. DOI:10.3321/j.issn:0250-3301.2007.04.029. PAN Y, ZHOU L X. Dynamics of dissolved organic matter and its effect on copper and cadmium activity in the contaminated wheat soil: field micro-plot trials[J].Environmental Science, 2007, 28(4):4859-4865.
[27] 咸翼松. 施用泥炭对土壤镉形态及其植物有效性的影响[D]. 杭州:浙江大学, 2008. XIAN Y S. Effects of peat application on chemical fractions and bioavailability of cadmium in contaminated soils [D]. Hangzhou: Zhejiang University, 2008.
[28] PARK J H, CHOPPALA G K, BOLAN N S, et al. Biochar reduces the bioavailability and phytotoxicity of heavy metals [J]. Plant and Soil, 2011, 348(1/2): 439-451. DOI:10.1007/s11104-011-0948-y.
[29] 占新华, 周立祥, 黄焕忠.城市污泥堆肥中水溶性有机物的理化特性变化[J].中国环境科学,2003,23(4):390-394. DOI:10.3321/j.issn:1000-6923.2003.04.013. ZHAN X H, ZHOU L X, HUANG H Z. The changes of physico-chemical character of dissolved organic matter in composting of municipal sludge[J].China Environmental Science, 2003,23(4):390-394.
[30] 杨园, 王艮梅. 杨树对镉胁迫的响应及抗性机制研究进展[J].世界林业研究, 2017, 30(4): 29-34. DOI:10.13348/j.cnki.sjlyyj.2017.0042.y. YANG Y, WANG G M. Poplar response to Cd stress and its resistance mechanism[J].World Forestry Research, 2017,30(4): 29-34.
[31] GRATÃO P L, POLLE A, LEA P J, et al. Making the life of heavy metal-stressed plants a little easier[J]. Functional Plant Biology, 2005, 32(6): 481-494. DOI:10.1071/fp05016.
[32] 杨园, 王艮梅, 曹莉, 等. 生物炭和猪粪堆肥对Cd污染土壤上黑麦草生理生化的影响[J].江苏农业科学, 2017,45(13): 196-200. DOI:10.15889/j.issn.1002-1302.2017.13.054. YANG Y, WANG G M, CAO L, et al. Effects of biochar and pig manure compost on physiological and biochemical characteristics of ryegrass in Cd contaminated soil[J]. Jiangsu Agricultural Science, 2017,45(13): 196-200.
[33] 廖兴国, 郭圣茂, 赖娴,等. 配方施肥对桔梗抗氧化酶活性的影响[J].贵州农业科学, 2014, 42(10): 144-147. DOI:10.3969/j.issn.1001- 3601. 2014.10.038. LIAO X G,GUO S M,LAI X, et al. Effects of formulated fertilization on antioxidant enzyme activities of Platycodon grandiflorum[J].Gui