南京林业大学学报(自然科学版) ›› 2019, Vol. 43 ›› Issue (04): 33-42.doi: 10.3969/j.issn.1000-2006.201810015
张 宁1,黄曜曜1,敖 妍1,2*,苏淑钗1,2,刘金凤3,张行杰3,刘觉非4
出版日期:
2019-07-22
发布日期:
2019-07-22
基金资助:
ZHANG Ning1, HUANG Yaoyao1, AO Yan1,2*, SU Shuchai1,2, LIU Jinfeng3, ZHANG Xingjie3, LIU Juefei4
Online:
2019-07-22
Published:
2019-07-22
摘要: 【目的】研究文冠果花芽分化过程外部形态变化与内部解剖结构的对应关系,并探究内源激素含量与花芽分化的关系,为文冠果花性别相关研究的适时采样与提高雌/雄花比例的调控技术提供参考。【方法】通过外部形态观测,结合石蜡切片技术,分析文冠果花芽分化内外部形态之间的对应关系。并采用高效液相色谱法(HPLC)对花芽分化过程中雌、雄能花的4种内源激素含量进行测定和比较,探究其与内源激素之间的关系。【结果】① 文冠果雌能花和雄能花在性别分化之前均为两性,雌能花的单核花粉粒在有丝分裂期呈液泡化衰败,雄能花大孢子母细胞四分体时期细胞停止分裂,性器官发育异常导致单性花的形成。此外,文冠果花芽分化内部结构与外部形态之间具有时序性对应关系。② 玉米素(zeatin,ZT)含量在花芽未分化期至雌蕊原基分化期逐渐上升,雌雄配子体形成时期,雌能花的ZT含量始终高于雄能花。雌能花雄蕊发育出现异常时的生长素(auxin,IAA)、胞落酸(abscisic acid,ABA)含量显著高于雄能花,赤霉素(gibberellin,GA)含量显著低于雄能花。说明高水平ZT促进花芽分化,利于雌能花的发育; 高水平的IAA和ABA具有促雌作用; 高水平的GA具有促雄效应。③ m(ABA)/m(IAA)、m(ABA)/m(GA)、m(ZT)/m(GA)在花芽未分化期至雌蕊原基分化期均处于较高水平,雄能花雌蕊发育异常时,其m(ABA)/m(IAA)和m(ZT)/m(IAA)显著高于雌能花。说明高水平的m(ABA)/m(IAA)、m(ABA)/m(GA)、m(ZT)/m(GA)有利于花芽分化; 高水平m(ABA)/m(IAA)、m(ZT)/m(IAA)对雄蕊发育有利。【结论】文冠果雌能花和雄能花各自对应另一性器官的败育导致了性别分化。花芽分化过程中,可以根据花芽外部形态判断内部分化时期。高水平的ZT含量、m(ABA)/m(IAA)、m(ABA)/m(GA)、m(ZT)/m(GA)利于花芽分化,高水平的m(ZT)、m(IAA)和m(ABA)利于雌能花发育,高水平的GA含量、m(ABA)/m(IAA)、m(ZT)/m(IAA)利于雄能花发育。
中图分类号:
张宁,黄曜曜,敖妍,等. 文冠果花芽分化过程及内源激素动态变化[J]. 南京林业大学学报(自然科学版), 2019, 43(04): 33-42.
ZHANG Ning, HUANG Yaoyao, AO Yan, SU Shuchai, LIU Jinfeng, ZHANG Xingjie, LIU Juefei. Flower bud differentiation and dynamic changes of endogenous hormone in Xanthoceras sorbifolium Bunge[J].Journal of Nanjing Forestry University (Natural Science Edition), 2019, 43(04): 33-42.DOI: 10.3969/j.issn.1000-2006.201810015.
[1] 蔡龙. 提高中国特有能源树种文冠果座率的技术研究[D].北京: 北京林业大学, 2012. CAI L. The technological study for improving the fruit rate of Xanthoceras sorbifolia Bunge, a unique energy species[D]. Beijing: Beijing Forestry University, 2012. [2] 敖妍. 不同地区文冠果群体种子含油率·产量变异规律[J].安徽农业科学, 2009, 37(25): 11967-11969. DOI:10.13989/j.cnki.0517-6611.2009.25.091. AO Y. Variation law of seed oil content and yield of Xanthoceras sorbifolium Bunge populations in different areas[J]. Journal of Anhui Agricultural Sciences, 2009, 37(25): 11967-11969. [3] 马芳, 王俊, 王姮, 等. 文冠果树花部形态与开花物候的研究[J]. 北方园艺, 2014, 325(22): 80-84. MA F, WANG J, WANG H, et al. Study on floral characters and flowering phenology in Xanthoceras sorbifolia Bunge[J]. Northern Horticulture, 2014, 325(22): 80-84. [4] 张敏, 王頔, 张雷, 等. 文冠果雌雄花发育过程形态结构比较[J]. 电子显微学报, 2012, 31(2): 154-162. DOI:10.3969/j.issn.1000-6281.2012.02.010. ZHANG M, WANG D, ZHANG L, et al. Female and male flower differentiation in Xanthoceras sorbifolium Bunge[J]. Journal of Chinese Electron Microscopy Society, 2012, 31(2): 154-162. [5] YOUNG T E, GIESLER-Lee J, GALLIE D R. Senescence-incluced expression of cytokinin reverses pistil abortion during maize flower development[J]. Plant Journal, 2004, 38:910-922. DOI:10.1111/j.1365-313x.2004.02093.x. [6] 王丽萍, 李志刚, 谭乐和, 等. 植物内源激素研究进展[J].安徽农业科学, 2011, 39(4): 1912-1914. DOI:10.13989/j.cnki.0517-6611.2011.04.150. WANG L P, LI Z G, TAN L H, et al. Research progress of plant endogeous hormone[J]. Journal of Anhui Agricultural Sciences, 2011, 39(4): 1912-1914. [7] ADHIKARI S, BANDYOPADHYAY T K, GHOSH P. Hormonal control of sex expression of cucumber(Cucumis sativus L.)with the identification of sex linked molecular marker [J]. The Nucleus, 2012, 55: 115-122.DOI:10.1007/s13237-012-0061-5. [8] 肖华山, 吕柳新, 陈志彤. 荔枝花芽分化过程中内源激素含量的动态变化[J]. 宁德师范学院学报(自然科学版), 2007, 19: 113-115.DOI:10.3969/j.issn.2095-2481.2007.02.001. XIAO H S, LV L X, CHEN Z T. Dynamic changes of endogenous hormone content during flower bud differentiation of litchi[J]. Journal of Ningde Teachers College(Natural Science), 2007, 19: 113-115. [9] HEGELE M, MANOCHAI P, NAPHROM D et al. Flowering in longan(Dimocarpus longan L.)induced by hormonal changes following KClO3 applications[J]. European Journal of Horticultural Science, 2008, 73: 49. [10] 金亚征, 姚太梅, 丁丽梅, 等. 果树花芽分化机理研究进展[J]. 北方园艺, 2013(7): 193-196. DOI:10.3969/j.issn.1003-5400.2014.09.264. JIN Y Z, YAO T M, DING L M, et al. Research progress on the mechanism of flower bud differentiation of fruit trees[J]. Northern Horticulture, 2013(7):193-196. [11] MO C M, TU D P, HUANG J, et al. Morphological and endogenous hormones characteristics of flower bud of Siraitia grosvenorii during its differentiation[J]. Acta Botanica Boreali Occidentalia Sinica, 2015, 35(1): 98-106. DOI:10.7606/j.issn.1000-4025.2015.01.0098. [12] GAO Y, LIU H, PEI D. Morphological characteristics and in situ auxin production during the histogenesis of staminate flowers in precocious walnut[J]. Journal of the American Society for Horticulturalence,2014, 139: 185-190. [12] 郭成圆. 板栗花芽分化及内源激素变化的研究[D]. 杨凌:西北农林科技大学, 2010. GUO C Y. Study on the flower bud differentiation and the change of endogenous hormone in chestnut[D]. Yangling: Northwest A & F University, 2010. [14] KONG L, ADERKAS Von P, ZAHARIA L I. Effects of exogenously applied gibberellins and thidiazuron on phytohormone profiles of long-shoot buds and cone gender determination in Lodgepole pine[J]. Journal of Plant Growth Regulation, 2016, 35(1):172-182.DOI:10.1007/s00344-015-9517-6. [15] 敖妍, 段劼, 于海燕, 等. 文冠果研究进展[J]. 中国农业大学学报, 2012, 17(6): 197-203. AO Y, DUAN J, YU H Y, et al. Research progress on Xanthoceras sorbifolia Bunge[J]. Journal of China Agricultural University, 2012, 17(6): 197-203. [16] 吴尚, 马履一, 段劼, 等. 文冠果花期和果期内源激素的动态变化规律[J]. 西北农林科技大学学报(自然科学版), 2017,45(4):111-118. DOI:10.13207/j.cnki.jnwafu.2017.04.016 WU S, MA L Y, DUAN J, et al. Changes of endogenous hormones in Xanthoceras sorbifolia Bunge at florescence and fruit periods[J]. Journal of Northwest A & F University(Natural Science Edition), 2017, 45(4): 111-118. [17] 漆小雪, 韦霄, 王熊军, 等. 金花茶花期内源激素含量的变化[J]. 江苏农业科学, 2013, 41(3): 141-144. DOI:10.3969/j.issn.1002-1302.2013.03.057. QI X X, WEI X, WANG X J, et al. Changes of source hormone content during Camellia sinensis[J]. Jiangsu Agricultural Sciences, 2013, 41(3): 141-144. [18] 魏永赞, 董晨, 王弋, 等. 荔枝花芽分化与花性别分化研究进展[J]. 广东农业科学, 2017, 44(7): 34-40. WEI Y Z, DONG C, WANG Y, et al. Advances in flower bud differentiation and flower sex determination in litchi[J]. Guangdong Agricultural Sciences, 2017, 44(7): 34-40. [19] 王宏国. 龙眼花性分化的细胞学机制研究[D]. 福州: 福建农林大学, 2008. WANG H G. Study on the cytology mechanism of flower sexual differentiation in longan [D]. Fuzhou: Fujian Agriculture And Forestry University, 2008. [20] 王平, 郑伟, 陈伟. 荔枝花性别分化过程的荧光显微观察[J]. 热带作物学报, 2010, 31(5): 740-744. DOI:10.3969/j.issn.1000-2561.2010.05.010 WANG P, ZHENG W, CHEN W. Fluorescence microscopy of the sex differentiation process of litchi flower[J]. Chinese Journal of Tropical Crops, 2010, 31(5):740-744. [21] 韩明慧, 彭方仁, 邓秋菊, 等. 薄壳山核桃雌雄花芽分化外部形态与内部结构的关系[J]. 南京林业大学学报(自然科学版), 2017, 41(6): 1-7. DOI:10.3969/j.issn.1000-2006.201703020. HAN M H, PENG F R, DENG Q J, et al. Observation of morphological and anatomical characters on staminate and pistillate flower differentiation in Carya illinoensis(Wangenh.)C. Koch[J]. Journal of Nanjing Forestry University(Natural Sciences Edition), 2017, 41(6): 1-7. [22] 王丽媛, 郭素娟. 板栗‘燕山早丰’雌花分化的解剖学研究[J]. 中南林业科技大学学报, 2017, 37(1): 43-47. DOI:10.14067/j.cnki.1673-923x.2017.01.008. WANG L Y, GUO S J. Study on anatomy of development of female flower in chestnut ‘Yanshanzaofeng’[J]. Journal of Central South University of Forestry & Technology, 2017, 37(1): 43-47. [23] 许自龙, 汪阳东, 陈益存, 等. 山鸡椒雄花花芽发育形态解剖特征观察[J]. 植物科学学报, 2017, 35(2): 152-163. DOI:10.11913/PSJ.2095-0837.2017.20152. XU Z L, WANG Y D, CHEN Y C, et al. Observation of the morphological and anatomical characteristics of male flower bud development in Litsea cubeba(Lour.)pess[J]. Plant Science Journal, 2017, 35(2): 152-163. [24] 胡盼, 王川, 王军辉, 等. 青海云杉花芽分化期内源激素含量的变化特征[J]. 西北植物学报, 2012, 32(3): 540-545. DOI:10.3969/j.issn.1000-4025.2012.03.016. HU P, WANG C, WANG J H, et al. Content changes of endogenous hormones during flower bud differentiation of Picea crassifolia[J]. Acta Botanica Boreali-Occidentalia Sinica, 2012, 32(3): 540-545. [25] 杨同文, 李潮海. 玉米性别决定的激素调控[J]. 植物学报, 2012, 47: 65-73. DOI:10.3724/SP.J.1259.2012.00065. YANG T W, LI C H. Hormone regulation of maize sex determination[J]. Bulletin of Botany, 2012,47: 65-73. [26] 焦竹青, 王振兴, 许培磊, 等. 山葡萄雄株花蕾性别转换的形态观察及其内源激素的变化[J]. 北方园艺, 2012(16): 5-8. JIAO Z Q, WANG Z X, XU P L, et al. Morphological observation of the sex conversion of male flower buds and changes of endogenous hormones in Mountain grape[J]. Northern Horticulture, 2012, 16: 5-8. [27] 肖华山, 吕柳新, 陈志彤. 荔枝花发育过程中雌雄蕊内源激素的动态变化[J]. 应用与环境生物学报, 2003(1): 11-15. DOI:10.3321/j.issn:1006-687X.2003.01.003. XIAO H S, LV L X, CHEN Z T. Dynamic changes of endogenous hormone in litchi(Litchi chinensis Sonn.)pistil and stamen during flower development[J]. Chinese Journal of Applied and Environmental Biology, 2003(1): 11-15. [28] BANKS J A. MicroRNA, sex determination and floral meristem determinacy in maize[J]. Genome Biol, 2008, 9: 204. DOI:10.1186/gb-2008-9-1-204. [29] CAO S Y, ZHANG J C, WEI L H. Studies on the changes of endogenous hormones in the differentiation period of flower bud in apple trees [J]. Journal of Fruit Science, 2000, 17(4): 244-248. DOI:10.13925/j.cnki.gsxb.2000.04.002. [30] CAO S Y, TANG Y Z. Effects of GA3 and PP333 on the apple flower bud differentiation course and contents of endogenous hormone [J]. Journal of Fruit Science, 2001, 18(6): 313-316. DOI:10.13925/j.cnki.gsxb.2001.06.001. [31] SHI J K, ZHANG W P, FAN W G, et al. Changes in endogenous hormones during the differentiation of female flower bud of ginkgo(G. biloba L.)[J]. Acta Horticulturae Sinica, 1999, 26(3): 194-195. [32] 孔冬梅. 激素对高等植物性别分化的调控研究进展[J].安徽农业科学, 2009, 37(12): 5352-5354. DOI:10.3969/j.issn.0517-6611.2009.12.013. KONG D M. Advances in studies on the regulation of hormones on sex differentiation in higher plants[J]. Journal of Anhui Agricultural Sciences, 2009, 37(12): 5352-5354. [33] KUMAR A, JAISWAL V S. Sex reversal and fruit formation on male plants of Carica papaya L. by ethrel and chlorflurenol [J]. Proceedings: Plant Sciences, 1984, 93(6): 635-641. [34] CHAILAKHYAN M K. Genetic and hormonal regulation of growth, flowering, and sex expression in plants [J]. American Journal of Botany, 1979, 66(6):717-736. [35] 惠文凯, 杨舒贻, 陈涵斌, 等. 赤霉素诱导麻疯树雌雄花分化的研究[J]. 南京林业大学学报(自然科学版), 2016,40(6): 175-179. DOI:10.3969/j.issn.1000-2006.2016.06.027. HUI W K, YANG S Y, CHEN H B, et al. Study on the differentiation of male and female flowers induced by gibberellin[J]. Journal of Nanjing Forestry University(Natural Sciences Edition), 2016, 40(6): 175-179. [36] LI T H, HUANG W D, MENG Z Q. Study on the mechanisms of flower bud induction in apple tree[J]. Acta Physiologica Sinica, 1996, 22(3): 251-257. [37] WANG Y H,FAN C H, SHEN X, et al. Changes in endogenous hormones during the flower bud differentiation of sweet cherry[J]. Acta Agriculturae Boreali Occidentalis Sinica, 2002, 11(1): 64-67. DOI:10.7606/j.issn.1004-1389.2002.1.020. [38] 曾骧. 果树生理学[M]. 北京: 北京农业大学出版社, 1992. ZENG X. Fruit tree physiology[M]. Beijing: Beijing Agricultural University Press, 1992. [39] DUAN N, JIA Y K, XU J, et al. Research progress on plant endogenous hormones [J]. Chinese Agricultural Science Bulletin, 2015, 31(2): 159-165. [40] ADHIKARI S, BANDYOPADHYAY T K, GHOSH P. Hormonal control of sex expression of cucumber(Cucumis sativus L.)with the identification of sex linked molecular marker[J]. The Nucleus, 2012, 55: 115-122. |
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