楸树嫩枝扦插生根发育及根系特征分析

doi:10.3969/j.issn.1000-2006.202003082

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南京林业大学学报（自然科学版） ›› 2020, Vol. 44 ›› Issue (6): 94-102.doi: 10.3969/j.issn.1000-2006.202003082

楸树嫩枝扦插生根发育及根系特征分析

王改萍(), 王良桂, 王晓聪, 张晨, 章雷, 刘彬

南京林业大学林学院,南方现代林业协同创新中心,江苏南京 210037

收稿日期:2020-03-28 修回日期:2020-07-17 出版日期:2020-11-30 发布日期:2020-12-07
基金资助:
国家重点研发计划(2017YFD0600604)

Dynamic characteristics of cutting rooting of Catalpa bungei with tender branches

WANG Gaiping(), WANG Lianggui, WANG Xiaocong, ZHANG Chen, ZHANG Lei, LIU Bin

Co-Innovation Center for the Sustainable Forestry in Southern China,College of Forestry, Nanjing Forestry University,Nanjing 210037,China

Received:2020-03-28 Revised:2020-07-17 Online:2020-11-30 Published:2020-12-07

摘要/Abstract

摘要：

【目的】楸树大多自花不育,苗木主要通过营养繁殖获得,而高质量楸树苗木缺乏,扦插育苗存在繁殖系数低、质量参差不一等问题,影响了楸树的大面积推广利用。笔者探索不同处理方式下楸树嫩枝生根及不定根形成特点,初步筛选合适的楸树嫩枝生根方法,为揭示楸树嫩枝扦插生根机理并解决楸树扦插困难提供参考。【方法】以楸树品种‘8611’为试验材料,采用两因素(生根剂类型、枝条部位)完全随机试验,观察插穗在扦插生根过程中的动态变化并进行生根阶段划分;采用石蜡切片法,对不同生根阶段的插穗进行解剖结构分析;利用根系扫描仪测定根系的相关指标,并对各指标数据进行相关性分析和多重比较,得出楸树最优生根处理,明确楸树生根类型及时间。【结果】楸树扦插生根过程可以分为4个阶段:扦插3~7 d为愈伤组织的形成阶段;扦插7~18 d为不定根的诱导阶段;扦插18~20 d为不定根的发生阶段;扦插20 d以后为不定根的伸长阶段。不同生根剂及枝条不同部位的插穗对嫩枝扦插生根存在明显影响。穗条在不同部位间,枝条上部的插穗最易生根,但易腐烂;枝条中部制成的插穗生根效果最佳。不同激素处理中,GGR-6处理的插穗最易生根,生根效果最佳。在不同激素处理下枝条不同部位的生根率表现为:1 000 mg/kg GGR-6处理的中部插穗生根效果最佳,生根率约92%。在形态解剖学观察中,未发现楸树‘8611’种存在潜伏根原基。【结论】楸树嫩枝扦插的生根时间约20 d,为诱导生根类型。采用嫩枝中部制成的插穗,通过1 000 mg/kg GGR-6处理时生根效果最佳。

关键词: 楸树, 嫩枝扦插, 解剖观察, 根系发育, 生根率

Abstract:

【Objective】Catalpa bungei is a precious wood tree species in China. Most C. bugein trees are self-sterile and their seedlings are mainly obtained through vegetative propagation. However, a low propagation coefficient and lack of high-quality cutting seedlings have restrained the development of C. bungei plantation. In order to explore the mechanism of C. bungei rooting and to select a suitable method for cutting rooting of C. bungei, the characteristics of root and principles of adventitious root formation were studied using different treatment methods.【Method】Using variety ‘8611’ of C. bungei as materials, a two factor (rooting agent, branch position) completely randomized design experiment was conducted in this study. The dynamic changes of cuttings and rooting stages were observed and divided during the process of rooting, and then the anatomical structure of cuttings at different rooting stages was analyzed using the paraffin section method. Finally, the related indexes of root system were measured using a root scanner and the correlation analysis and multiple comparison of various index data were carried out to obtain the best root handle.【Result】The rooting process of C. bungei cuttings was divided into four stages: 3-7 days as a callus induction period; 7-18 days as a callus and adventitious roots formation stage; 18-20 days as a large-scale form of adventitious roots of cutting stage; and after 20 days as an adventitious roots elongation period. Different hormones and branch positions had significant effects on shoot rooting. Cuttings made from the upper position of branches easily took root, but were perishable; however, cuttings from the middle position of branches had the best rooting effectiveness. The cuttings treated with GGR-6 were easier in taking root and had the best root formation. Comprehensively, the best treatment was that of the cuttings from the middle position of branches with 1 000 mg/kg GGR-6, in which the rooting rate was approximately 92%. No latent root primordium was found based on morphological and anatomical observations and it is speculated that variety ‘8611’ belongs to the induced root primordium type.【Conclusion】The rooting time of C. bungei was about 20 days, which is likely to be the time of induced rooting. The optimal treatment for cutting rooting of C. bungei was the middle position of the tender branches treated with 1 000 mg/kg GGR-6.

Key words: Catalpa bungei, shoot cutting, anatomy observation, root development, rooting rate

中图分类号:

S722.8 ⁺9

王改萍,王良桂,王晓聪,等. 楸树嫩枝扦插生根发育及根系特征分析[J]. 南京林业大学学报（自然科学版）, 2020, 44(6): 94-102.

WANG Gaiping, WANG Lianggui, WANG Xiaocong, ZHANG Chen, ZHANG Lei, LIU Bin. Dynamic characteristics of cutting rooting of Catalpa bungei with tender branches[J].Journal of Nanjing Forestry University (Natural Science Edition), 2020, 44(6): 94-102.DOI: 10.3969/j.issn.1000-2006.202003082.

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参考文献 34

[1]	张锦. 国粹楸树材貌绝伦[J].中国林业, 2004(6):34.
	ZHANG J. Catalpa bungei, the quintessence of China, has excellent wood appearance[J]. Forestry of China, 2004(6):34.
[2]	郭明. 木王楸树[J].国土绿化, 2002(2):29.
	GUO M. The king of wood-Catalpa bungei[J]. Land Greening, 2002(2):29.
[3]	张瑞波, 张本强, 赵金娟, 等. 几个楸树无性系引种造林试验[J]. 山东林业科技, 2011,41(4):67-69.
	ZHANG R B, ZHANG B Q, ZHAO J J, et al. Study on the introduction of Catalpa bungei clones in Shangdong Province[J]. Journal of Shandong Forestry Science and Technology, 2011,41(4):67-69.DOI: 10.3969/j.issn.1002-2724.2011.04.021.
[4]	韩东花, 杨桂娟, 肖遥, 等. 楸树无性系早期生长变异和优选[J]. 林业科学研究, 2019,32(4):96-104.
	HAN D H, YANG G J, XIAO Y, et al. Study on early growth variation of Catalpa bungei clones and optimization[J]. Forest Research, 2019,32(4):96-104. DOI : 10.13275/j.cnki.lykxyj.2019.04.013.
[5]	梁有旺, 彭方仁, 王顺才. 楸树嫩枝扦插试验初报[J]. 林业科技开发, 2006,20(1):67-69.
	LIANG Y W, PENG F R, WANG S C. A preliminary study on green shot cutting of Catalpa bungei[J]. China Forestry Science and Technology, 2006,20(1):67-69. DOI: 10.3969/j.issn.1000-8101.2006.01.022.
[6]	王改萍, 岑显超, 彭方仁, 等. 不同楸树品种的抗旱性鉴定[J]. 浙江林学院学报, 2009,26(6):815-821.
	WANG G P, CEN X C, PENG F R, et al. Drought resistance in seedlings of Catalpa bungei cultivars[J]. Journal of Zhejiang Forestry College, 2009,26(6):815-821.DOI: 10.3969/j.issn.2095-0756.2009.06.009.
[7]	AHKAMI A H, LISCHEWSKI S, HAENSCH K T, et al. Molecular physiology of adventitious root formation in Petunia hybrida cuttings:involvement of wound response and primary metabolism[J]. New Phytologist, 2009,181(3):613-625. DOI: 10.1111/j.1469-8137.2008.02704.x.
[8]	张恩亮, 马玲玲, 杨如同, 等. IBA诱导楸树嫩枝扦插不定根发育的转录组分析[J]. 林业科学, 2018,54(5):48-61.
	ZHANG E L, MA L L, YANG R T, et al. Transcriptome profiling of IBA-induced adventitious root formation in softwood cuttings of Catalpa bungei ‘Yu-1’[J]. Scientia Silvae Sinicae, 2018,54(5):48-61.DOI: 10.11707/j.1001-7488.20180506.
[9]	韦东山, 张秋良, 常金宝. 美国薄荷嫩枝扦插繁殖及生根机理研究[J]. 南京林业大学学报(自然科学版), 2018,42(5):39-45.
	WEI D S, ZHANG Q L, CHANG J B. A study on mechanism of propagation and shoot cutting of Monarda didyma[J]. J Nanjing For Univ(Nat Sci Ed), 2018,42(5):39-45. DOI: 10.3969/j.issn.1000-2006.201801045.
[10]	梁有旺, 杜旭华, 王顺财, 等. 楸树嫩枝扦插生根的主要影响因子分析[J]. 植物资源与环境学报, 2008,17(4):46-50.
	LIANG Y W, DU X H, WANG S C, et al. Analysis of main influence factors on rooting of twig cutting of Catalpa bungei[J]. Journal of Plant Resources and Environment, 2008,17(4):46-50.
[11]	王凯基, 倪德祥. 几种木本植物组织培养的愈伤组织形成和器官再生[J]. 植物学报, 1981,23(2):97-103.
	WANG K J, NI D X. Callus formation and organ regeneration in tissue culture of woody plants[J]. Journal of Integrative Plant Biology, 1981,23(2) : 97-103.
[12]	CONTESSA C, VALENTINI N, BOTTA R. Decreasing the concentration of IBA or combination with ethylene inhibitors improve bud retention in semi-hardwood cuttings of hazelnut cultivar ‘Tonda Gentile delle Langhe’[J]. Scientia Horticulturae, 2011,131:103-106.DOI: 10.1016/j.scienta.2011.09.029.
[13]	卢乃会, 陈杰, 韩成友. 不同激素处理对母生枝条扦插繁殖的影响[J]. 安徽农业科学, 2016,44(13):217-219.
	LU N H, CHEN J, HAN C Y. Effects of different hormone treatments on cutting propagation in Homalium hainanense[J]. Journal of Anhui Agricultural Sciences, 2016,44(13):217-219. DOI: 10.13989/j.cnki.0517-6611.2016.13.069.
[14]	郭素娟. 林木扦插生根的解剖学及生理学研究进展[J]. 北京林业大学学报, 1997(4):66-71.
	GUO S J. Progress of study on rooting anatomy and physiology of forest tree cuttings[J]. Journal of Beijing Forestry University, 1997(4):66-71. DOI : 10.13332/j.1000-1522.1997.04.011.
[15]	林艳, 詹亚光, 刘玉喜, 等. 白桦嫩枝扦插不定根形成的解剖观察[J]. 东北林业大学学报, 1996(3):15-19.
	LIN Y, ZHAN Y G, LIU Y X, et al. The anatomical study on formation of adventitious root of softwood cutting in Betula platyphylia[J]. Journal of Northeast Forestry University, 1996(3):15-19. DOI : 10.13759/j.cnki.dlxb.1996.03.009.
[16]	曹凡. 美国山核桃扦插繁殖技术及生根机理研究[D]. 南京:南京林业大学, 2015.
	CAO F. Studies on cutting propagat ion techniques and its rooting mechanism of Carya illinoinensis[D]. Nanjing:Nanjing Forestry University, 2015.
[17]	宋晓深, 刘玉香, 肖复明, 等. 棱角山矾扦插生根解剖学机理研究[J]. 江西林业科技, 2012 ( 5):14-16.
	SONG X S, LIU Y X, XIAO F M, et al. Rooting mechanism with the method of anatomy for stem cuttings of Symplocos tetragona[J]. Jiangxi Forestry Science and Technology, 2012 ( 5):14-16.DOI: 10.16259/j.cnki.36-1342/s.2012.05.013.
[18]	哈德曼H T. 植物繁殖原理和技术[M]. 郑开文, 吴应祥,李嘉乐,等,译.北京:中国林业出版社, 1985.
	HARDMAN H T. Principle and technology of plant reproduction[M]. ZHENG K W, WU Y X, LI J L, et al, Translated .Beijing: China Forestry Publishing House, 1985.
[19]	梁晓静, 朱昌叁, 安家成, 等. 小叶芳樟扦插育苗技术及其不定根形成机理[J]. 广西林业科学, 2020,49(1):7-12.
	LIANG X J, ZHU C S, AN J C, et al. Cuttage technology and adventitious rooting mechanism of Ciuuamomum camphora var. linaloolifera[J]. Guangxi Forestry Science, 2020,49(1):7-12.DOI: 10.19692/j.cnki.gfs.2020.01.002.
[20]	森下义朗. 植物扦插理论与技术[M]. 北京: 中国林业出版社, 1988.
	YOSHIRO M. Theory and technology of plant cutting[M]. Beijing: China Forestry Publishing House, 1988.
[21]	梁玉堂. 树木营养繁殖原理和技术[M]. 北京: 中国林业出版社, 1993.
	LIANG Y T, Principles and techniques of vegetative propagation of trees[M]. Beijing: China Forestry Publishing House, 1993.
[22]	张新宇. 磁化处理对楸树嫩枝扦插生根的影响[D]. 泰安:山东农业大学, 2017.
	ZHANG X Y. Effect of magnetic treatment on rooting of semi lignified twigs of Catalpa bungei[D]. Tai’an: Shandong Agricultural University, 2017.
[23]	邓华平, 王正超. 不同扦插设施条件下金丝楸嫩枝扦插的研究[J]. 河北林果研究, 2009,24(4):381-384.
	DENG H P, WANG Z C. Research on rooting of twig cutting of Catalpa bungei C.A. Meyer under different cutting facilities[J]. Hebei Journal of Forestry and Orchard Research, 2009,24(4):381-384.
[24]	魏黔春, 江泽平, 刘建锋, 等. 侧柏古树扦插试验及插穗营养物质变化[J]. 南京林业大学学报(自然科学版), 2020,44(1):63-71.
	WEI Q C, JIANG Z P, LIU J F, et al. Effects of several factors on rooting of cutting propagation of ancient Platycladus orierrtalias and the changes of nutritive material[J]. J Nanjing For Univ (Nat Sci Ed), 2020,44(1):63-71. DOI: 10.3969/j.issn.1000-2006.201903057.
[25]	吕运舟, 樊厚宏, 施士争, 等. 元宝枫嫩枝扦插试验[J]. 江苏林业科技, 2019,46(3):10-13.
	LYU Y Z, FAN H H, SHI S Z, et al. Study on softwood cutting technique of Acer truncatum[J]. Journal of Jiangsu Forestry Science &Technology, 2019,46(3):10-13. DOI: 10.3969/j.issn.1001-7380.2019.03.003.
[26]	许晓岗. 垂丝海棠、楸子的扦插生根机理研究[D]. 南京:南京林业大学, 2006.
	XU X G. The research of rooting mechanism of Malus halliana & Malus prunifolia cutting[D]. Nanjing:Nanjing Forestry University, 2006.
[27]	孟丙南. 四倍体刺槐扦插技术优化及生根机理研究[D]. 北京:北京林业大学, 2010.
	MENG B N. Study on optimizing of cuttage propagation and mechanism of rooting in tetraploid Robiniapseudoacacia[D]. Beijing:Beijing Forestry University, 2010.
[28]	Hartmann H T. Effect of season of collecting,dolebutyric acid,and pre-planting storage treatment on rooting of Marianna plum,peach,and quince hardwood cuttings[J]. American Society for Horticultural Science, 1958 ( 71):57-66.
[29]	袁利利. 华北五角枫扦插繁殖技术及生根机理的研究[D]. 泰安:山东农业大学, 2012.
	YUAN L L. The studies on the propagation techniques and rooting mechanism of stem cuttings of Acer truncatum Bunge[D]. Tai’an:Shandong Agricultural University, 2012.
[30]	王东光. 闽楠嫩枝扦插繁殖技术及生根机理研究[D]. 北京:中国林业科学研究院, 2013.
	WANG D G. Soft cutting propagation techniques and rooting mechanism of Phoebe bournei (Hemsl.) Yang[D]. Beijing: China Academy of Forestry Sciences, 2013.
[31]	李恩佳. 不同基质、生根剂及其质量浓度对美国红火球紫薇嫩枝扦插生根的交互影响[J]. 江苏林业科技, 2018,45(1):10-13.
	LI E J. Orthogonal experimental study on cutting of Lagerstroemia indica[J]. Journal of Jiangsu Forestry Science &Technology, 2018,45(1):10-13.DOI: 10.3969/j.issn.1001-7380.2018.01.003.
[32]	刘建明, 吕跃东, 姚颖. 不同处理方法对不同沙棘品种扦插生根的影响[J]. 森林工程, 2018,34(1):13-15.
	LIU J M, LV Y D, YAO Y. Influence of different processing methods on different varieties of Hippophae rhamnoides Linn. cutting and cutting[J]. Forest Engineering, 2018,34(1):13-15. DOI: 10.16270/j.cnki.slgc.2018.01.003.
[33]	赵翔, 李清莹, 姜清彬, 等. 不同基质和促根剂对灰木莲嫩枝扦插生根的影响[J]. 南京林业大学学报(自然科学版), 2019,43(2):23-30.
	ZHAO X, LI Q Y, JIANG Q B, et al. Effects of substrates and rooting regulators on rooting of cuttings in Mahglietia cohifera Dandy[J]. J Nangjing For Univ (Nat Sci Ed), 2019,43(2):23-30. DOI: 10.3969/j.issn.1000-2006.201805051.
[34]	仝伯强, 赵永军, 杨海平, 等. 我国北方楸树资源(Sect. Sinocatalpa)繁育技术研究进展[J]. 安徽农业科学, 2019,47(14):1-3,17.
	TONG B Q, ZHAO Y J, YANG H P, et al. Research progress on breeding techniques of Sect.Sinocatalpa in northern China[J]. Journal of Anhui Agricultural Sciences, 2019,47(14):1-3,17.

处理 treatment	Z1/cm				Z2/cm				Z3/个				Z4/cm²
处理 treatment	上 upper	中 middle	下 lower	上 upper		中 middle	下 lower	上 upper		中 middle	下 lower	上 upper		中 middle	下 lower
GGR-6	124.72± 5.46 a	139.81± 13.19 a	108.12± 6.77 a	14.08± 2.68 a		17.01± 3.44 a	13.73± 1.84 a	17.24± 2.57 a		15.61± 0.92 a	9.92± 0.25 b	47.49± 20.22 a		55.78± 15.67 a	56.58± 13.39 a
m(NAA): m(IBA)=2:1	113.13± 2.04 b	98.65± 2.56 b	105.79± 18.71 a	16.05± 1.05 a		11.96± 0.91 b	13.38± 5.18 a	12.50± 2.11 b		13.12± 0.78 b	12.68± 1.31 a	39.22± 10.47 a		51.23± 8.06 a	57.60± 8.15 a
CK	95.14± 1.53 c	123.59± 14.64 a	96± 24.15 b	12.67± 0.88 a		11.33± 0.67 b	14.29± 0.61 a	11.85± 0.61 b		13.85± 0.25 b	11.64± 0.75 ab	43.84± 17.66 a		48.11± 65.43 a	20.06± 6.41 a
F	31.156	47.000	10.921	8.807		10.139	5.921	8.334		5.812	6.091	0.942		3.130	4.843
P	<0.01	<0.01	0.020	0.026		0.024	0.063	0.019		0.039	0.036	0.441		0.117	0.056
处理 treatment	Z5/mm				Z6/个				Z7				Z8/%
处理 treatment	上 upper	中 middle	下 lower	上 upper		中 middle	下 lower	上 upper		中 middle	下 lower	上 upper		中 middle	下 lower
GGR-6	1.02± 0.45 a	6.73± 1.74 a	2.87± 0.62 a	580.56± 69.21 a		405.94± 48.20 a	376.54± 10.45 a	2.42± 0.49 a		3.18± 0.40 a	2.59± 2.00 a	84.44± 6.77 a		92.00± 1.05 a	85.75± 2.04 a
m(NAA): m(IBA)=2:1	1.40± 0.43 a	4.09± 1.42 b	2.16± 0.43 a	382.70± 27.13 a		287.17± 20.84 a	213.72± 15.07 a	1.75± 1.93 b		3.06± 0.75 a	2.30± 1.75 a	75.97± 8.52 b		81.07± 2.02 a	85.21± 7.13 a
CK	1.31± 0.06 a	6.38± 1.20 a	1.68± 0.34 a	552.67± 65.73 a		591.00± 34.76 a	204.00± 64.02 a	2.31± 0.24 a		2.36± 0.20 b	1.44± 0.27 a	61.33± 5.30 ab		77.28± 4.34 b	73.25± 2.16 b
F	2.760	10.922	0.695	2.795		1.573	2.130	17.063		5.826	14.467	11.773		17.901	2.227
P	0.141	0.018	0.535	0.139		0.282	0.200	0.003		0.039	0.005	0.008		0.003	0.184

因素factor	水平lever	Z1/cm	Z2/cm	Z3/个	Z4/cm²	Z5/mm	Z6/个	Z7	Z8/%
部位 position	上 upper	111.00± 14.90 ab	14.27± 1.69 a	13.86± 2.94 ab	43.84± 4.14 a	1.24± 0.20 ab	505.31± 107.10 a	2.16± 0.36 b	73.91± 11.69 b
	中 middle	120.68± 20.73 a	13.43± 3.11 a	13.99± 1.28 a	51.71± 3.86 a	5.73± 1.43 a	428.04± 153.12 ab	2.87± 0.44 a	83.45± 7.64 a
	下 lower	103.30± 6.43 b	13.80± 0.45 a	11.41± 1.39 b	44.75± 21.39 a	2.24± 0.60 b	264.75± 96.93 b	2.11± 0.59 b	81.40± 7.07 ab
生根剂 rooting agent	GGR-6	124.22± 15.85 a	14.94± 1.80 a	14.05± 3.84 a	56.18± 5.03 a	3.54± 2.91 a	454.35± 110.29 a	2.73± 0.40 a	87.40± 4.04 a
	m(NAA): m(IBA)=2:1	105.86± 7.24 ab	13.80± 2.07 a	12.73± 0.32 a	54.42± 9.33 a	2.55± 1.39 b	294.53± 84.73 b	2.37± 0.66 ab	80.75± 4.62 ab
	CK	104.91± 16.18 b	12.76± 1.48 a	12.45± 1.22 a	37.34± 15.11 a	3.12± 2.83 a	449.22± 213.23 ab	2.04± 0.52 b	70.62± 8.29 b

指标index	Z8	Z1	Z2	Z3	Z4	Z5	Z6	Z7
Z8	1
Z1	0.35	1
Z2	-0.17	0.48	1
Z3	0.32	0.71^*	0.22	1
Z4	0.50	0.41	-0.08	0.186	1
Z5	0.75^*	0.59	-0.29	0.25	0.41	1
Z6	-0.07	0.44	-0.19	0.44	0.19	0.14	1
Z7	0.81^*	0.43	-0.05	0.35	0.80^**	0.62	0.19	1

楸树嫩枝扦插生根发育及根系特征分析

Dynamic characteristics of cutting rooting of Catalpa bungei with tender branches

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