花楸属直脉组7种/变种基因组大小及叶表皮微形态特征的分类学意义

邱靖; 李嘉宝; 朱大海; 陈昕

doi:10.12302/j.issn.1000-2006.2021100096

邱靖, 李嘉宝, 朱大海, 陈昕

南京林业大学学报（自然科学版） ›› 2023, Vol. 47 ›› Issue (3) : 77-86.

PDF(5005 KB)

国家林草科技领军期刊
中国精品科技期刊
中国高校百佳科技期刊
江苏省新闻出版政府奖期刊奖
RCCSE林学权威期刊（A+）
CSCD核心期刊
Scopus数据库收录期刊
中文核心期刊
SCD核心期刊

作者加群：102861116

微信公众号：南京林业大学学报

高级检索

PDF(5005 KB)

南京林业大学学报（自然科学版） ›› 2023, Vol. 47 ›› Issue (3) : 77-86. DOI: 10.12302/j.issn.1000-2006.2021100096

研究论文

花楸属直脉组7种/变种基因组大小及叶表皮微形态特征的分类学意义

邱靖 ¹^,^△ ,
李嘉宝 ^△ ,
朱大海 ³ ,
陈昕 ¹^,^*

作者信息 +

Taxonomic implications of genome sizes and micromorphological characteristics of leaf epidermis of species in Sorbus Sect. Alnifoliae

QIU Jing ¹ ,
LI Jiabao ,
ZHU Dahai ³ ,
CHEN Xin ¹^,^*

Author information +

文章历史 +

摘要

【目的】通过对花楸属直脉组(Sorbus Sect. Alnifoliae)5种及具有分类争议的2变种的基因组大小和叶表皮微形态研究,探讨其分类学意义以及基因组大小和细胞大小之间的相关性。【方法】基于流式细胞术、光学和电子显微镜分别对研究对象的基因组大小、叶表皮细胞和气孔的质量和数量性状进行测定、分析。【结果】基因组大小测定显示所有分类群均为极小基因组,大小介于神农架花楸(S. yuana)的1.348 pg至长果花楸(S. zahlbruckneri)的1.586 pg之间;虽均为二倍体,但分类群间基因组大小差异显著。光学显微镜下叶表皮细胞形状兼有多边形和无规则形,或者仅无规则形,垂周壁式样有平直状、弧状和波状;扫描电镜下表皮纹饰有近光滑、局部加厚、脊状、网纹、褶皱等类型,蜡质纹饰有壳状、颗粒状、线状、片状等;4个分类群的下表皮具带状单毛。气孔器均为无规则型,分布于下表皮脉间区;保卫细胞具明显的环形外缘或无。表皮细胞面积、气孔宽度及面积、气孔密度、气孔指数等指标在分类群间差异显著,基因组大小同表皮细胞面积、气孔长宽及面积呈正相关,同气孔密度和气孔指数呈负相关。【结论】基因组大小、叶表皮微形态质量和数量性状可为7个分类群的鉴定提供依据,不支持将棱果花楸(S. alnifolia var. angulata)和裂叶水榆花楸(S. japonica)作为日本花楸(S. alnifolia var. lobulata)的异名处理,建议恢复棱果花楸和裂叶水榆花楸的名称。

Abstract

【Objective】Genome sizes and micromorphological characteristics of leaf epidermis in five species representing Sorbus Sect. Alnifoliae together with two taxonomically controversial varieties were studied. The purpose of this study was to explore the taxonomic significance and the correlation between genome size and cell size. 【Method】The genome size was estimated by flow cytometry. Qualitative and quantitative traits of leaf epidermis were studied by optical and scanning electron microscopy【Result】The flow cytometric genome size estimation revealed that seven investigated taxa possessed very small genomes. The nuclear DNA content varied from 1.348 pg in S. yuana to 1.586 pg in S. zahlbruckneri. Though all are diploids, significant variation of genome size was observed among taxa. Polygonal-irregular or irregular epidermal cell shape, straight, arched or undulate of anticlinal walls, were determined using light microscopy. Patterns of cuticular ornamentation such as smooth, thickened areas, ridges, reticulate and folds and epicuticular wax types such as crusts, granules, threads and platelets were observed by scanning electron microscopy. Simple single ribbon-like trichomes are located on the lower epidermis of four taxa. Stomatal apparatuses are anomocytic and confined to areole on abaxial, not adaxial, epidermis. Guard cells with or without obvious annular outer edges. Significant variations in epidermal cell area, stomatal width, area, density and stomatal index, were observed among the taxa. There was a positive relationship between genome size and stomatal length, width, area and epidermal cell area and a negative relationship with stomatal density and stomatal index. 【Conclusion】Genome sizes, qualitative and quantitative traits of leaf epidermis allow clear delimitation and identification of the seven taxa. The treatments of S. alnifolia var. angulata and S. alnifolia var. lobulata as synonyms of S. japonica are not supported and the latter two names are suggested to be reinstated here.

导出引用

邱靖, 李嘉宝, 朱大海, 等. 花楸属直脉组7种/变种基因组大小及叶表皮微形态特征的分类学意义[J]. 南京林业大学学报（自然科学版）. 2023, 47(3): 77-86 https://doi.org/10.12302/j.issn.1000-2006.2021100096

QIU Jing, LI Jiabao, ZHU Dahai, et al. Taxonomic implications of genome sizes and micromorphological characteristics of leaf epidermis of species in Sorbus Sect. Alnifoliae[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2023, 47(3): 77-86 https://doi.org/10.12302/j.issn.1000-2006.2021100096

中图分类号： S792.25;Q943

参考文献

列表( 原文顺序 | 文献年度倒序 | 文中引用次数倒序 ) 可视化分析

[1]	PHIPPS J B, ROBERTSON K R, SMITH P G, et al. A checklist of the subfamily maloideae (Rosaceae)[J]. Can J Bot, 1990, 68(10):2209-2269.DOI:10.1139/b90-288. 本文引用 [1]

[2]	DECAISNE M J. Mémoirs sur le famille des Pomacées[J]. Nouvelles Archives du Muséum d’Histoire Naturelle, 1874, 10: 113-192. 本文引用 [1]

[3]	ALDASORO J J, AEDO C, GARMENDIA F M, et al. Revision of Sorbus subgenera Aria and Torminaria (Rosaceae-maloideae)[J]. Syst Bot Monogr, 2004, 69:1.DOI:10.2307/25027918. 本文引用 [4]

[4]	CAMPBELL C S, EVANS R C, MORGAN D R, et al. Phylogeny of subtribe Pyrinae (Formerly The Maloideae,Rosaceae):limited resolution of a complex evolutionary history[J]. Plant Syst Evol, 2007, 266(1/2):119-145.DOI:10.1007/s00606-007-0545-y. 本文引用 [1]

[5]	POTTER D, ERIKSSON T, EVANS R C, et al. Phylogeny and classification of Rosaceae[J]. Plant Syst Evol, 2007, 266(1/2):5-43.DOI:10.1007/s00606-007-0539-9. 本文引用 [1]

[6]	ZHANG S D, JIN J J, CHEN S Y, et al. Diversification of Rosaceae since the Late Cretaceous based on plastid phylogenomics[J]. New Phytol, 2017, 214(3):1355-1367.DOI:10.1111/nph.14461. 本文引用 [1]

[7]	王国勋, 张明理. 应用核DNA ITS序列探讨广义花楸属(Sorbus L.)属下系统关系[J]. 园艺学报, 2011, 38(12):2387-2394. WANG G X, ZHANG M L. A molecular phylogeny of Sorbus(Rosaceae)based on ITS sequence[J]. Acta Hortic Sin, 2011, 38(12):2387-2394.DOI:10.16420/j.issn.0513-353x.2011.12.001. 本文引用 [1]

[8]	LU L T, SPONGBERG S A. Sorbus Linnaeus[M]// WU Z Y, RAVEN P H, HONG D Y. Flora of China, Vol. 9. Rosaceae. Beijing:Science Press; St Louis: Missouri Botanical Garden Press, 2003: 144-170. 本文引用 [1]

[9]	MCALLISTER H. The genus Sorbus-mountain ssh and other rowans[M]. London: Royal Botanical Gardens, 2005:252. 本文引用 [1]

[10]	俞德浚, 关克俭. 中国蔷薇科植物分类之研究(一)[J]. 植物分类学报, 1963, 8(3): 202-236. YU D J, GUAN K J. Studies on the taxonomy of Rosaceae in China(1)[J]. Journal of Systematics and Evolution, 1963, 8(3): 202-234. 本文引用 [1]

[11]	LEITCH A R, LEITCH I J. Ecological and genetic factors linked to contrasting genome dynamics in seed plants[J]. New Phytol, 2012, 194(3):629-646.DOI:10.1111/j.1469-8137.2012.04105.x. 本文引用 [1]

[12]	ROBERTS A V, GLADIS T, BRUMME H. DNA amounts of roses (Rosa L.) and their use in attributing ploidy levels[J]. Plant Cell Rep, 2009, 28(1):61-71.DOI:10.1007/s00299-008-0615-9. 本文引用 [1]

[13]	ROTHLEUTNER J J, FRIDDLE M W, CONTRERAS R N. Ploidy levels,relative genome sizes,and base pair composition in Cotoneaster[J]. J Amer Soc Hort Sci, 2016, 141(5):457-466.DOI:10.21273/jashs03776-16. 本文引用 [2]

[14]	HAJRUDINOVIC A, FRAJMAN B, SCHÖNSWETTER P, et al. Towards a better understanding of polyploid Sorbus (Rosaceae) from Bosnia and Herzegovina (Balkan Peninsula),including description of a novel,tetraploid apomictic species[J]. Bot J Linn Soc, 2015, 178(4):670-685.DOI:10.1111/boj.12289. 本文引用 [1]

[15]	HAJRUDINOVIC A, SILJAK-YAKOVLEV S, BROWN S C, et al. When sexual meets apomict:Genome size,ploidy level and reproductive mode variation of Sorbus aria S.L. and S.austriaca (Rosaceae) in Bosnia and Herzegovina[J]. Ann Bot, 2015, 116(2):301-312.DOI:10.1093/aob/mcv093. 本文引用 [1]

[16]	PELLICER J, CLERMONT S, HOUSTON L, et al. Cytotype diversity in the Sorbus complex (Rosaceae) in Britain:sorting out the puzzle[J]. Ann Bot, 2012, 110(6):1185-1193.DOI:10.1093/aob/mcs185. 本文引用 [2]

[17]	LI J B, ZHU K L, WANG Q, et al. Genome size variation and karyotype diversity in eight taxa of Sorbus sensu stricto (Rosaceae) from China[J]. Comp Cytogenet, 2021, 15(2):137-148.DOI:10.3897/CompCytogen. 本文引用 [2]

[18]	HAMZEH’EE B, ATTAR F, ASSAREH M, et al. Comparative micromorphological characteristics of lower surface of leaf epidermis and seed surface in two series of Crataegus L.(Rosaceae) and their taxonomical role[J]. IRAN J BOT, 2016, 22(2):125-137. 本文引用 [1]

[19]

杨晨璇, 李楚然, 李璐, 等. 中国特有属藤山柳属(猕猴桃科)植物的叶表皮形态及其分类学意义[J]. 西北植物学报, 2020, 40(6):997-1010.

YANG

C X

, LI

C R

, LI

, et al. Leaf epidermis morphology of the Chinese endemic genus Clematoclethra (Actinidiaceae) and its taxonomic significance[J]. Acta Bot Boreali Occidentalia Sin, 2020, 40(6):997-1010.DOI:10.7606/j.issn.1000-4025.2020.06.0997.

本文引用 [1]

[20]	PODWYSZYNSKA M, KRUCZYNSKA D, MACHLANSKA A, et al. Nuclear DNA content and ploidy level of apple cultivars including Polish ones in relation to some morphological traits[J]. Acta Biol Cracoviensia S Bot, 2016, 58(1):81-93.DOI:10.1515/abcsb-2016-0008. 本文引用 [1]

[21]	BEAULIEU J M, LEITCH I J, PATEL S, et al. Genome size is a strong predictor of cell size and stomatal density in angiosperms[J]. New Phytol, 2008, 179(4):975-986.DOI:10.1111/j.1469-8137.2008.02528.x. 本文引用 [2]

[22]	VESELY P, SMARDA P, BUREŠ P, et al. Environmental pressures on stomatal size may drive plant genome size evolution:evidence from a natural experiment with Cape geophytes[J]. Ann Bot, 2020, 126(2):323-330.DOI:10.1093/aob/mcaa095. 本文引用 [2]

[23]	FAIZULLAH L, MORTON J A, HERSCH-GREEN E I, et al. Exploring environmental selection on genome size in angiosperms[J]. Trends Plant Sci, 2021, 26(10):1039-1049.DOI:10.1016/j.tplants.2021.06.001. 本文引用 [1]

[24]	LOUREIRO J, RODRIGUEZ E, DOLEZEL J, et al. Two new nuclear isolation buffers for plant DNA flow cytometry:a test with 37 species[J]. Ann Bot, 2007, 100(4):875-888.DOI:10.1093/aob/mcm152. 本文引用 [1]

[25]	DILCHER D L. Approaches to the identification of angiosperm leaf remains[J]. Bot Rev, 1974, 40(1):1-157.DOI:10.1007/BF02860067. 本文引用 [1]

[26]	王宇飞, 陶君容. 植物角质层分析术语新体系[J]. 植物学通报, 1991, 26(4):6-13. WANG Y F, TAO J R. An introduction to a new system of terminology for plant cuticular analysis[J]. Chin Bull Bot, 1991, 26(4):6-13. 本文引用 [1]

[27]	BARTHLOTT W, NEINHUIS C, CUTLER D, et al. Classification and terminology of plant epicuticular waxes[J]. Bot J Linn Soc, 1998, 126(3):237-260.DOI:10.1006/bojl.1997.0137. 本文引用 [2]

[28]	DICKSON E E, ARUMUGANATHAN K, KRESOVICH S, et al. Nuclear DNA content variation within the Rosaceae[J]. Am J Bot, 1992, 79(9):1081-1086.DOI:10.1002/j.1537-2197.1992.tb13697.x. 本文引用 [1]

[29]	BURGESS M B, CUSHMAN K R, DOUCETTE E T, et al. Effects of apomixis and polyploidy on diversification and geographic distribution in Amelanchier (Rosaceae)[J]. Am J Bot, 2014, 101(8):1375-1387.DOI:10.3732/ajb.1400113. 本文引用 [1]

[30]	TALENT N, DICKINSON T A. Polyploidy in Crataegus and Mespilus(Rosaceae,Maloideae):Evolutionary inferences from flow cytometry of nuclear DNA amounts[J]. Can J Bot, 2005, 83(10):1268-1304.DOI:10.1139/b05-088. 本文引用 [1]

[31]	SENNIKOV A, KURTTO A. A phylogenetic checklist of Sorbus S.L. (Rosaceae) in Europe[J]. Memoranda Societatis pro Fauna et Flora Fennica, 2017, 93:1-78. 本文引用 [1]

[32]	曾妮, 张建茹, 常朝阳. 中国蔷薇属植物叶表皮微形态特征及其系统学意义[J]. 广西植物, 2017, 37(2):169-185. ZENG N, ZHANG J R, CHANG Z Y. Micromorphological characteristics of leaf epidemis and systematic significance of Rosa L.from China[J]. Guihaia, 2017, 37(2):169-185.DOI:10.11931/guihaia.gxzw201602005. 本文引用 [1]

[33]	ZAMANI A, ATTAR F, CIVEYREL L. Leaf epidermis characters of Iranian Pyrus L. (Rosaceae) and their taxonomic implications[J]. Genetic Resources and Crop Evolution, 2017, 64: 159-176. DOI:10.1007/s10722-015-0341-4. 本文引用 [1]

[34]	GANEVA T, UZUNOVA K. Comparative leaf epidermis study in species of genus Malus Mill. (Rosaceae)[J]. Botanica Serbica, 2010, 34(1): 45-49. 本文引用 [1]

[35]	郗连连, 李嘉宝, 朱凯琳. 花楸属3种植物的基因组大小与叶气孔特征分析[J]. 植物科学学报, 2020, 38(1): 32-38. XI L L, LI J B, ZHU K L. Variation in genome size and stomatal teaits among three Sorbus species.[J]. Plant Science Journal, 2020, 38(1): 32-38. DOI:10.11913/PSJ.2095-0837.2020.10032. 本文引用 [1]